| 1 | A guided tour into subcellular colocalization analysis in light microscopy | Journal of Microscopy | 2006 | 4,072 |
| 2 | The efficiency of systematic sampling in stereology and its prediction* | Journal of Microscopy | 1987 | 2,768 |
| 3 | Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. SHORT COMMUNICATION | Journal of Microscopy | 2000 | 2,500 |
| 4 | The unbiased estimation of number and sizes of arbitrary particles using the disector | Journal of Microscopy | 1984 | 1,979 |
| 5 | Measurement of co‐localization of objects in dual‐colour confocal images | Journal of Microscopy | 1993 | 1,665 |
| 6 | Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object | Journal of Microscopy | 2002 | 1,535 |
| 7 | The quantitative analysis of thin specimens | Journal of Microscopy | 1975 | 1,437 |
| 8 | A new method for the model‐independent assessment of thickness in three‐dimensional images | Journal of Microscopy | 1997 | 1,421 |
| 9 | Stereology of arbitrary particles* | Journal of Microscopy | 1986 | 1,240 |
| 10 | The efficiency of systematic sampling in stereology — reconsidered | Journal of Microscopy | 1999 | 1,048 |
| 11 | Notes on the estimation of the numerical density of arbitrary profiles: the edge effect | Journal of Microscopy | 1977 | 1,000 |
| 12 | Multiphoton microscopy in life sciences | Journal of Microscopy | 2000 | 887 |
| 13 | Estimation of surface area from vertical sections | Journal of Microscopy | 1986 | 820 |
| 14 | Reconstruct: a free editor for serial section microscopy | Journal of Microscopy | 2005 | 693 |
| 15 | The correlation averaging of a regularly arranged bacterial cell envelope protein | Journal of Microscopy | 1982 | 679 |
| 16 | Fluorescence lifetime imaging – techniques and applications | Journal of Microscopy | 2012 | 609 |
| 17 | Resin development for electron microscopy and an analysis of embedding at low temperature* | Journal of Microscopy | 1982 | 499 |
| 18 | FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells | Journal of Microscopy | 2004 | 466 |
| 19 | Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index | Journal of Microscopy | 1993 | 454 |
| 20 | Tissue shrinkage and unbiased stereological estimation of particle number and size* | Journal of Microscopy | 2001 | 430 |
| 21 | The quantitative morphology of anisotropic trabecular bone | Journal of Microscopy | 1974 | 427 |
| 22 | The nucleator | Journal of Microscopy | 1988 | 386 |
| 23 | Optimizing sampling efficiency of stereological studies in biology: or ‘Do more less well!‘ | Journal of Microscopy | 1981 | 385 |
| 24 | Application of cryoultramicrotomy to immunocytochemistry | Journal of Microscopy | 1986 | 384 |
| 25 | Stereological estimation of the volume‐weighted mean volume of arbitrary particles observed on random sections* | Journal of Microscopy | 1985 | 379 |
| 26 | Three‐dimensional reconstruction from a single‐exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli | Journal of Microscopy | 1987 | 362 |
| 27 | New direct observations of asphalts and asphalt binders by scanning electron microscopy and atomic force microscopy | Journal of Microscopy | 1996 | 355 |
| 28 | Application of the Cavalieri principle and vertical sections method to lung: estimation of volume and pleural surface area | Journal of Microscopy | 1988 | 344 |
| 29 | Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae | Journal of Microscopy | 2000 | 337 |
| 30 | Electron microscopy of frozen water and aqueous solutions | Journal of Microscopy | 1982 | 324 |
| 31 | Orthogonal‐plane fluorescence optical sectioning: Three‐dimensional imaging of macroscopic biological specimens | Journal of Microscopy | 1993 | 323 |
| 32 | Origin and significance of the SEM cathodoluminescence from zircon | Journal of Microscopy | 1993 | 297 |
| 33 | New approach to optical diffraction tomography yielding a vector equation of diffraction tomography and a novel tomographic microscope | Journal of Microscopy | 2002 | 288 |
| 34 | Three-dimensional analysis of porous BaTiO3 ceramics using FIB nanotomography | Journal of Microscopy | 2004 | 281 |
| 35 | Bitumen morphologies by phase-detection atomic force microscopy | Journal of Microscopy | 2006 | 278 |
| 36 | VITRIFICATION OF PURE WATER FOR ELECTRON MICROSCOPY | Journal of Microscopy | 1981 | 276 |
| 37 | Total number of neurons and glial cells in human brain nuclei estimated by the disector and the fractionator | Journal of Microscopy | 1988 | 275 |
| 38 | Determination of membrane thickness distribution from orthogonal intercepts | Journal of Microscopy | 1979 | 274 |
| 39 | Estimation of surface area and length with the orientator | Journal of Microscopy | 1990 | 273 |
| 40 | Soft X-ray spectroscopy from image sequences with sub-100 nm spatial resolution | Journal of Microscopy | 2000 | 272 |
| 41 | Transmission EBSD from 10 nm domains in a scanning electron microscope | Journal of Microscopy | 2012 | 272 |
| 42 | Cell‐death assessment by fluorescent and nonfluorescent cytosolic and nuclear staining techniques | Journal of Microscopy | 2014 | 269 |
| 43 | Sampling designs for stereology* | Journal of Microscopy | 1981 | 261 |
| 44 | I5M: 3D widefield light microscopy with better than 100 nm axial resolution | Journal of Microscopy | 1999 | 259 |
| 45 | Preparation of animal tissues for surface‐scanning electron microscopy | Journal of Microscopy | 1969 | 250 |
| 46 | Confocal imaging of glutathione redox potential in living plant cells | Journal of Microscopy | 2008 | 243 |
| 47 | Drying cells for SEM, AFM and TEM by hexamethyldisilazane: a study on hepatic endothelial cells | Journal of Microscopy | 1997 | 241 |
| 48 | Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser | Journal of Microscopy | 2003 | 241 |
| 49 | Confocal scanning light microscopy with high aperture immersion lenses | Journal of Microscopy | 1979 | 236 |
| 50 | The isector: a simple and direct method for generating isotropic, uniform random sections from small specimens | Journal of Microscopy | 1992 | 236 |