# | Title | Journal | Year | Citations |
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1 | Rapid and automated tetrazolium-based colorimetric assay for the detection of anti-HIV compounds | Journal of Virological Methods | 1988 | 1,644 |
2 | Genogroup-specific PCR primers for detection of Norwalk-like viruses | Journal of Virological Methods | 2002 | 717 |
3 | A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus | Journal of Virological Methods | 2006 | 679 |
4 | Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV | Journal of Virological Methods | 2004 | 591 |
5 | Quantification of lymphocytic choriomeningitis virus with an immunological focus assay in 24- or 96-well plates | Journal of Virological Methods | 1991 | 446 |
6 | NASBATM isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection | Journal of Virological Methods | 1991 | 417 |
7 | Development of a TaqMan® PCR assay with internal amplification control for the detection of African swine fever virus | Journal of Virological Methods | 2003 | 392 |
8 | A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses | Journal of Virological Methods | 2006 | 357 |
9 | J. Virol. Methods | Journal of Virological Methods | 1996 | 352 |
10 | Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses | Journal of Virological Methods | 2006 | 348 |
11 | Generation of VSV pseudotypes using recombinant ΔG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines | Journal of Virological Methods | 2010 | 347 |
12 | Real-time RT-PCR for norovirus screening in shellfish | Journal of Virological Methods | 2005 | 342 |
13 | Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR | Journal of Virological Methods | 1999 | 337 |
14 | Development and application of a capsid VP1 (region D) based reverse transcription PCR assay for genotyping of genogroup I and II noroviruses | Journal of Virological Methods | 2004 | 327 |
15 | A simple method for cloning the complete begomovirus genome using the bacteriophage φ29 DNA polymerase | Journal of Virological Methods | 2004 | 321 |
16 | Identification and subtyping of avian influenza viruses by reverse transcription-PCR | Journal of Virological Methods | 2001 | 320 |
17 | Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control | Journal of Virological Methods | 2002 | 314 |
18 | Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA | Journal of Virological Methods | 2010 | 306 |
19 | Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses | Journal of Virological Methods | 2005 | 286 |
20 | Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens | Journal of Virological Methods | 2006 | 282 |
21 | Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments | Journal of Virological Methods | 2007 | 280 |
22 | A primer pair for amplifying part of the genome of all potyvirids by RT-PCR | Journal of Virological Methods | 1997 | 276 |
23 | Serological detection of hepatitis B virus genotypes by ELISA with monoclonal antibodies to type-specific epitopes in the preS2-region product | Journal of Virological Methods | 1999 | 276 |
24 | Study on the resistance of severe acute respiratory syndrome-associated coronavirus | Journal of Virological Methods | 2005 | 271 |
25 | Generation of a doxycycline-inducible KSHV producer cell line of endothelial origin: Maintenance of tight latency with efficient reactivation upon induction | Journal of Virological Methods | 2011 | 270 |
26 | A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay | Journal of Virological Methods | 1998 | 266 |
27 | Optimized large-scale production of high titer lentivirus vector pseudotypes | Journal of Virological Methods | 2004 | 259 |
28 | A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection | Journal of Virological Methods | 1992 | 256 |
29 | Solid-phase enzyme-linked immunosorbent assay for hepatitis E virus IgG and IgM antibodies utilizing recombinant antigens and synthetic peptides | Journal of Virological Methods | 1992 | 245 |
30 | Development of a TaqMan® RT-PCR assay without RNA extraction step for the detection and quantification of African Chikungunya viruses | Journal of Virological Methods | 2005 | 245 |
31 | Flow cytometric detection of viruses | Journal of Virological Methods | 2000 | 244 |
32 | Detection of norovirus (GI, GII), Sapovirus and astrovirus in fecal samples using reverse transcription single-round multiplex PCR | Journal of Virological Methods | 2003 | 236 |
33 | High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodies | Journal of Virological Methods | 2009 | 235 |
34 | Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA | Journal of Virological Methods | 2011 | 234 |
35 | A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes | Journal of Virological Methods | 1994 | 231 |
36 | Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors | Journal of Virological Methods | 2006 | 230 |
37 | A polymerase chain reaction assay adapted to plum pox potyvirus detection | Journal of Virological Methods | 1991 | 227 |
38 | Detection and diagnosis of parapoxvirus by the polymerase chain reaction | Journal of Virological Methods | 2000 | 225 |
39 | New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay | Journal of Virological Methods | 1995 | 218 |
40 | Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever | Journal of Virological Methods | 2005 | 216 |
41 | A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens | Journal of Virological Methods | 2008 | 215 |
42 | Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro | Journal of Virological Methods | 2011 | 210 |
43 | Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay | Journal of Virological Methods | 2002 | 209 |
44 | Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) | Journal of Virological Methods | 2008 | 207 |
45 | Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs | Journal of Virological Methods | 2004 | 206 |
46 | Antineoplasic activity of parvoviruses | Journal of Virological Methods | 1991 | 201 |
47 | Pretreatment to avoid positive RT-PCR results with inactivated viruses | Journal of Virological Methods | 2002 | 198 |
48 | Rapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses | Journal of Virological Methods | 2007 | 198 |
49 | Role of virus strain dm conventional and enhanced measles plaque neutralization test | Journal of Virological Methods | 1981 | 194 |
50 | Comparison of RNA hybridization, hemagglutination assay, titration of infectious virus and immunofluorescence as methods for monitoring influenza virus replication in vitro | Journal of Virological Methods | 1998 | 194 |