| # | Title | Journal | Year | Citations |
|---|
| 1 | Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor | Biotechnology and Bioengineering | 2009 | 2,628 |
| 2 | Hydrogels as extracellular matrix mimics for 3D cell culture | Biotechnology and Bioengineering | 2009 | 2,244 |
| 3 | Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems | Biotechnology and Bioengineering | 2004 | 1,537 |
| 4 | Rules for optimization of biocatalysis in organic solvents | Biotechnology and Bioengineering | 1987 | 1,516 |
| 5 | Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction | Biotechnology and Bioengineering | 2005 | 1,321 |
| 6 | Use of the upflow sludge blanket (USB) reactor concept for biological wastewater treatment, especially for anaerobic treatment | Biotechnology and Bioengineering | 1980 | 1,157 |
| 7 | Optknock: A bilevel programming framework for identifying gene knockout strategies for microbial strain optimization | Biotechnology and Bioengineering | 2003 | 1,112 |
| 8 | A mathematical model for the continuous culture of microorganisms utilizing inhibitory substrates | Biotechnology and Bioengineering | 1968 | 1,078 |
| 9 | Nanocomposite hydrogels for biomedical applications | Biotechnology and Bioengineering | 2014 | 916 |
| 10 | Fermentative butanol production by clostridia | Biotechnology and Bioengineering | 2008 | 909 |
| 11 | Ionic liquid‐mediated selective extraction of lignin from wood leading to enhanced enzymatic cellulose hydrolysis | Biotechnology and Bioengineering | 2009 | 844 |
| 12 | Potential of silver nanoparticle-coated polyurethane foam as an antibacterial water filter | Biotechnology and Bioengineering | 2005 | 813 |
| 13 | Alginate as immobilization material: I. Correlation between chemical and physical properties of alginate gel beads | Biotechnology and Bioengineering | 1989 | 798 |
| 14 | The challenge of enzyme cost in the production of lignocellulosic biofuels | Biotechnology and Bioengineering | 2012 | 792 |
| 15 | Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types | Biotechnology and Bioengineering | 2003 | 777 |
| 16 | Model of the anaerobic metabolism of the biological phosphorus removal process: Stoichiometry and pH influence | Biotechnology and Bioengineering | 1994 | 733 |
| 17 | Photocrosslinked hyaluronic acid hydrogels: Natural, biodegradable tissue engineering scaffolds | Biotechnology and Bioengineering | 2003 | 721 |
| 18 | Bacterial polyhydroxyalkanoates | Biotechnology and Bioengineering | 2000 | 699 |
| 19 | Effects of biofilm structures on oxygen distribution and mass transport | Biotechnology and Bioengineering | 1994 | 687 |
| 20 | Extraction chemistry of fermentation product carboxylic acids | Biotechnology and Bioengineering | 1986 | 646 |
| 21 | A review of the current state of biodiesel production using enzymatic transesterification | Biotechnology and Bioengineering | 2009 | 646 |
| 22 | Diffusion characteristics of substrates in Ca-alginate gel beads | Biotechnology and Bioengineering | 1984 | 626 |
| 23 | Medical biofilms | Biotechnology and Bioengineering | 2008 | 623 |
| 24 | Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose | Biotechnology and Bioengineering | 2004 | 598 |
| 25 | Improved fuel cell and electrode designs for producing electricity from microbial degradation | Biotechnology and Bioengineering | 2003 | 595 |
| 26 | The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels | Biotechnology and Bioengineering | 1977 | 593 |
| 27 | Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment | Biotechnology and Bioengineering | 2008 | 590 |
| 28 | Growth ofSaccharomyces cerevisiae is controlled by its limited respiratory capacity: Formulation and verification of a hypothesis | Biotechnology and Bioengineering | 1986 | 579 |
| 29 | Room-temperature ionic liquids as replacements for organic solvents in multiphase bioprocess operations | Biotechnology and Bioengineering | 2000 | 543 |
| 30 | Establishment ofFUT8 knockout Chinese hamster ovary cells: An ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity | Biotechnology and Bioengineering | 2004 | 537 |
| 31 | Adriamycin, 14-hydroxydaimomycin, a new antitumor antibiotic fromS. Peucetius var.caesius | Biotechnology and Bioengineering | 1969 | 534 |
| 32 | Opportunities for renewable bioenergy using microorganisms | Biotechnology and Bioengineering | 2008 | 533 |
| 33 | Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step | Biotechnology and Bioengineering | 2006 | 526 |
| 34 | A multispecies biofilm model | Biotechnology and Bioengineering | 1986 | 525 |
| 35 | Kinetics of product inhibition in alcohol fermentation | Biotechnology and Bioengineering | 1968 | 521 |
| 36 | Fractionating recalcitrant lignocellulose at modest reaction conditions | Biotechnology and Bioengineering | 2007 | 519 |
| 37 | The influence of high substrate concentrations on microbial kinetics | Biotechnology and Bioengineering | 1970 | 510 |
| 38 | Skin tissue generation by laser cell printing | Biotechnology and Bioengineering | 2012 | 509 |
| 39 | Simultaneous COD, nitrogen, and phosphate removal by aerobic granular sludge | Biotechnology and Bioengineering | 2005 | 507 |
| 40 | Butanol production from agricultural residues: Impact of degradation products onClostridium beijerinckii growth and butanol fermentation | Biotechnology and Bioengineering | 2007 | 505 |
| 41 | Dynamic modeling of the central carbon metabolism ofEscherichia coli | Biotechnology and Bioengineering | 2002 | 500 |
| 42 | Plasmid-encoded protein: The principal factor in the “metabolic burden” associated with recombinant bacteria | Biotechnology and Bioengineering | 1990 | 497 |
| 43 | Biorefining of softwoods using ethanol organosolv pulping: Preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products | Biotechnology and Bioengineering | 2005 | 493 |
| 44 | Dynamical model development and parameter identification for an anaerobic wastewater treatment process | Biotechnology and Bioengineering | 2001 | 485 |
| 45 | Metabolic flux distributions inCorynebacterium glutamicum during growth and lysine overproduction | Biotechnology and Bioengineering | 1993 | 484 |
| 46 | Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays | Biotechnology and Bioengineering | 2005 | 480 |
| 47 | Proton transport inside the biofilm limits electrical current generation by anode‐respiring bacteria | Biotechnology and Bioengineering | 2008 | 471 |
| 48 | Estimation of diffusion coefficients of proteins | Biotechnology and Bioengineering | 1980 | 465 |
| 49 | Large-scale biodiesel production from microalgaChlorella protothecoides through heterotrophic cultivation in bioreactors | Biotechnology and Bioengineering | 2007 | 465 |
| 50 | 3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs | Biotechnology and Bioengineering | 2019 | 464 |
