| 1 | Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor | Biotechnology and Bioengineering | 2009 | 2,520 |
| 2 | Hydrogels as extracellular matrix mimics for 3D cell culture | Biotechnology and Bioengineering | 2009 | 2,218 |
| 3 | Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems | Biotechnology and Bioengineering | 2004 | 1,488 |
| 4 | Rules for optimization of biocatalysis in organic solvents | Biotechnology and Bioengineering | 1987 | 1,454 |
| 5 | Group‐specific primer and probe sets to detect methanogenic communities using quantitative real‐time polymerase chain reaction | Biotechnology and Bioengineering | 2005 | 1,274 |
| 6 | A mathematical model for the continuous culture of microorganisms utilizing inhibitory substrates | Biotechnology and Bioengineering | 1968 | 1,083 |
| 7 | Use of the upflow sludge blanket (USB) reactor concept for biological wastewater treatment, especially for anaerobic treatment | Biotechnology and Bioengineering | 1980 | 1,082 |
| 8 | Nanocomposite hydrogels for biomedical applications | Biotechnology and Bioengineering | 2014 | 992 |
| 9 | Optknock: A bilevel programming framework for identifying gene knockout strategies for microbial strain optimization | Biotechnology and Bioengineering | 2003 | 985 |
| 10 | Fermentative butanol production by clostridia | Biotechnology and Bioengineering | 2008 | 870 |
| 11 | Ionic liquid‐mediated selective extraction of lignin from wood leading to enhanced enzymatic cellulose hydrolysis | Biotechnology and Bioengineering | 2009 | 818 |
| 12 | The challenge of enzyme cost in the production of lignocellulosic biofuels | Biotechnology and Bioengineering | 2012 | 798 |
| 13 | Potential of silver nanoparticle-coated polyurethane foam as an antibacterial water filter | Biotechnology and Bioengineering | 2005 | 782 |
| 14 | Alginate as immobilization material: I. Correlation between chemical and physical properties of alginate gel beads | Biotechnology and Bioengineering | 1989 | 775 |
| 15 | Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types | Biotechnology and Bioengineering | 2003 | 747 |
| 16 | Bacterial polyhydroxyalkanoates | Biotechnology and Bioengineering | 1996 | 710 |
| 17 | Model of the anaerobic metabolism of the biological phosphorus removal process: Stoichiometry and pH influence | Biotechnology and Bioengineering | 1994 | 691 |
| 18 | Effects of biofilm structures on oxygen distribution and mass transport | Biotechnology and Bioengineering | 1994 | 684 |
| 19 | Photocrosslinked hyaluronic acid hydrogels: Natural, biodegradable tissue engineering scaffolds | Biotechnology and Bioengineering | 2003 | 675 |
| 20 | Extraction chemistry of fermentation product carboxylic acids | Biotechnology and Bioengineering | 1986 | 635 |
| 21 | A review of the current state of biodiesel production using enzymatic transesterification | Biotechnology and Bioengineering | 2009 | 620 |
| 22 | Diffusion characteristics of substrates in Ca-alginate gel beads | Biotechnology and Bioengineering | 1984 | 611 |
| 23 | Medical biofilms | Biotechnology and Bioengineering | 2008 | 596 |
| 24 | Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment | Biotechnology and Bioengineering | 2008 | 589 |
| 25 | Improved fuel cell and electrode designs for producing electricity from microbial degradation | Biotechnology and Bioengineering | 2003 | 572 |
| 26 | Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose | Biotechnology and Bioengineering | 2004 | 568 |
| 27 | Growth ofSaccharomyces cerevisiae is controlled by its limited respiratory capacity: Formulation and verification of a hypothesis | Biotechnology and Bioengineering | 1986 | 565 |
| 28 | The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels | Biotechnology and Bioengineering | 1977 | 562 |
| 29 | Kinetics of product inhibition in alcohol fermentation | Biotechnology and Bioengineering | 1968 | 519 |
| 30 | Adriamycin, 14-hydroxydaimomycin, a new antitumor antibiotic fromS. Peucetius var.caesius | Biotechnology and Bioengineering | 1969 | 519 |
| 31 | Skin tissue generation by laser cell printing | Biotechnology and Bioengineering | 2012 | 517 |
| 32 | Room-temperature ionic liquids as replacements for organic solvents in multiphase bioprocess operations | Biotechnology and Bioengineering | 2000 | 512 |
| 33 | Opportunities for renewable bioenergy using microorganisms | Biotechnology and Bioengineering | 2008 | 511 |
| 34 | Simultaneous COD, nitrogen, and phosphate removal by aerobic granular sludge | Biotechnology and Bioengineering | 2005 | 504 |
| 35 | 3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs | Biotechnology and Bioengineering | 2019 | 503 |
| 36 | A multispecies biofilm model | Biotechnology and Bioengineering | 1986 | 502 |
| 37 | Dynamical model development and parameter identification for an anaerobic wastewater treatment process | Biotechnology and Bioengineering | 2001 | 495 |
| 38 | Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step | Biotechnology and Bioengineering | 2006 | 494 |
| 39 | Fractionating recalcitrant lignocellulose at modest reaction conditions | Biotechnology and Bioengineering | 2007 | 494 |
| 40 | The influence of high substrate concentrations on microbial kinetics | Biotechnology and Bioengineering | 1970 | 487 |
| 41 | Butanol production from agricultural residues: Impact of degradation products onClostridium beijerinckii growth and butanol fermentation | Biotechnology and Bioengineering | 2007 | 476 |
| 42 | Establishment ofFUT8 knockout Chinese hamster ovary cells: An ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity | Biotechnology and Bioengineering | 2004 | 471 |
| 43 | Plasmid‐encoded protein: The principal factor in the “metabolic burden” associated with recombinant bacteria | Biotechnology and Bioengineering | 1990 | 465 |
| 44 | Biorefining of softwoods using ethanol organosolv pulping: Preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products | Biotechnology and Bioengineering | 2005 | 465 |
| 45 | The membrane bioreactor: A novel tool to grow anammox bacteria as free cells | Biotechnology and Bioengineering | 2008 | 458 |
| 46 | A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports | Biotechnology and Bioengineering | 1998 | 457 |
| 47 | Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays | Biotechnology and Bioengineering | 2005 | 456 |
| 48 | Estimation of diffusion coefficients of proteins | Biotechnology and Bioengineering | 1980 | 455 |
| 49 | Dynamic modeling of the central carbon metabolism ofEscherichia coli | Biotechnology and Bioengineering | 2002 | 453 |
| 50 | Large-scale biodiesel production from microalgaChlorella protothecoides through heterotrophic cultivation in bioreactors | Biotechnology and Bioengineering | 2007 | 449 |
