| # | Title | Journal | Year | Citations |
|---|
|
| 1 | A new mathematical model for relative quantification in real-time RT-PCR | Nucleic Acids Research | 2001 | 29,318 |
| 2 | The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments | Clinical Chemistry | 2009 | 12,959 |
| 3 | Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines | Journal of Extracellular Vesicles | 2018 | 7,596 |
| 4 | Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR | Nucleic Acids Research | 2002 | 6,526 |
| 5 | Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations | Biotechnology Letters | 2004 | 4,273 |
| 6 | RNA integrity and the effect on the real-time qRT-PCR performance | Molecular Aspects of Medicine | 2006 | 1,237 |
| 7 | Defining ‘T cell exhaustion’ | Nature Reviews Immunology | 2019 | 996 |
| 8 | EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research | Nature Methods | 2017 | 959 |
| 9 | TOX reinforces the phenotype and longevity of exhausted T cells in chronic viral infection | Nature | 2019 | 648 |
| 10 | Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper | Journal of Extracellular Vesicles | 2017 | 589 |
| 11 | Sirtuin 1 and Sirtuin 3: Physiological Modulators of Metabolism | Physiological Reviews | 2012 | 575 |
| 12 | Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR | Biotechnology Letters | 2006 | 495 |
| 13 | Dietary synbiotics reduce cancer risk factors in polypectomized and colon cancer patients | American Journal of Clinical Nutrition | 2007 | 484 |
| 14 | Low-frequency repetitive transcranial magnetic stimulation of the motor cortex in writer’s cramp | Neurology | 1999 | 417 |
| 15 | Standardized determination of real-time PCR efficiency from a single reaction set-up | Nucleic Acids Research | 2003 | 376 |
| 16 | Transcription Factor IRF4 Promotes CD8+ T Cell Exhaustion and Limits the Development of Memory-like T Cells during Chronic Infection | Immunity | 2017 | 368 |
| 17 | Gas chromatographic analysis of fatty acid methyl esters | Biomedical Applications | 1995 | 315 |
| 18 | Precursor exhausted T cells: key to successful immunotherapy? | Nature Reviews Immunology | 2020 | 288 |
| 19 | Shear Stress Insensitivity of Endothelial Nitric Oxide Synthase Expression as a Genetic Risk Factor for Coronary Heart Disease | Circulation Research | 2004 | 280 |
| 20 | The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 | Clinical Chemistry | 2020 | 279 |
| 21 | Phytoestrogens Modulate Binding Response of Estrogen Receptors α and β to the Estrogen Response Element | Journal of Agricultural and Food Chemistry | 2003 | 248 |
| 22 | Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation | Cell Metabolism | 2019 | 244 |
| 23 | Genetic variability in the SNCA gene influences α‐synuclein levels in the blood and brain | FASEB Journal | 2008 | 237 |
| 24 | Human Imaging With Photon Counting–Based Computed Tomography at Clinical Dose Levels | Investigative Radiology | 2016 | 226 |
| 25 | mRNA and microRNA quality control for RT-qPCR analysis | Methods | 2010 | 220 |
| 26 | Comparison of Reverse Transcriptases in Gene Expression Analysis | Clinical Chemistry | 2004 | 210 |
| 27 | Expression and Signaling of Group I Metabotropic Glutamate Receptors in Astrocytes and Microglia | Journal of Neurochemistry | 1999 | 201 |
| 28 | Regulation of Corpus Luteum Function in Cattle - an Overview | Reproduction in Domestic Animals | 2004 | 195 |
| 29 | TCF1+ hepatitis C virus-specific CD8+ T cells are maintained after cessation of chronic antigen stimulation | Nature Communications | 2017 | 190 |
| 30 | Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next‐generation sequencing | Journal of Extracellular Vesicles | 2018 | 189 |
| 31 | The fate of trenbolone acetate and melengestrol acetate after application as growth promoters in cattle: environmental studies. | Environmental Health Perspectives | 2001 | 187 |
| 32 | Dietary fat and gut microbiota interactions determine diet-induced obesity in mice | Molecular Metabolism | 2016 | 183 |
| 33 | The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism | Microbiome | 2018 | 183 |
| 34 | Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding proteins 1, 2 and 3 in the bovine species | Domestic Animal Endocrinology | 2002 | 176 |
| 35 | Meltome atlas—thermal proteome stability across the tree of life | Nature Methods | 2020 | 174 |
| 36 | Expression and Tissue Concentration of Vascular Endothelial Growth Factor, Its Receptors, and Localization in the Bovine Corpus Luteum During Estrous Cycle and Pregnancy1 | Biology of Reproduction | 2000 | 155 |
| 37 | Short-term changes of mRNA expression of various inflammatory factors and milk proteins in mammary tissue during LPS-induced mastitis | Domestic Animal Endocrinology | 2004 | 151 |
| 38 | Prospective, randomized controlled trial onLactobacillus rhamnosusin infants with moderate to severe atopic dermatitis | British Journal of Dermatology | 2006 | 150 |
| 39 | Fractionized Milk Composition During Removal of Colostrum and Mature Milk | Journal of Dairy Science | 2003 | 137 |
| 40 | Nuclear magnetic resonance and mass spectrometry-based milk metabolomics in dairy cows during early and late lactation | Journal of Dairy Science | 2010 | 135 |
| 41 | Tissue-specific expression pattern of estrogen receptors (ER): Quantification of ERalpha and ERbeta mRNA with real-time RT-PCRNote | Apmis | 2001 | 130 |
| 42 | Individual variability in physiological adaptation to metabolic stress during early lactation in dairy cows kept under equal conditions | Journal of Animal Science | 2008 | 126 |
| 43 | Shp-2 Is Dispensable for Establishing T Cell Exhaustion and for PD-1 Signaling In Vivo | Cell Reports | 2018 | 125 |
| 44 | Inhibition of real-time RT–PCR quantification due to tissue-specific contaminants | Molecular and Cellular Probes | 2004 | 122 |
| 45 | Biochemistry and physiology of anabolic hormones used for improvement of meat production | Apmis | 2001 | 114 |
| 46 | Expression and localization of fibroblast growth factor (FGF) family members during the final growth of bovine ovarian follicles | Molecular Reproduction and Development | 2004 | 113 |
| 47 | Oxytocin Stimulates Progesterone Release from Microdialyzed Bovine Corpus Luteum in Vitro1 | Biology of Reproduction | 1991 | 111 |
| 48 | Evidence for Oxytocin Receptors in Cultured Bovine Luteal Cells1 | Biology of Reproduction | 1992 | 110 |
| 49 | Expression of Hyaluronan Synthases and Corresponding Hyaluronan Receptors Is Differentially Regulated During Oocyte Maturation in Cattle1 | Biology of Reproduction | 2003 | 109 |
| 50 | Ovarian function in ruminants | Domestic Animal Endocrinology | 2005 | 107 |
