| # | Title | Journal | Year | Citations |
|---|
| 1 | Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants | Plant Methods | 2005 | 1,290 |
| 2 | The advantages and limitations of trait analysis with GWAS: a review | Plant Methods | 2013 | 1,229 |
| 3 | Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs | Plant Methods | 2007 | 1,048 |
| 4 | Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method | Plant Methods | 2009 | 750 |
| 5 | A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes | Plant Methods | 2011 | 741 |
| 6 | PGA: a software package for rapid, accurate, and flexible batch annotation of plastomes | Plant Methods | 2019 | 660 |
| 7 | Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system | Plant Methods | 2013 | 515 |
| 8 | Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization | Plant Methods | 2007 | 438 |
| 9 | Hyperspectral image analysis techniques for the detection and classification of the early onset of plant disease and stress | Plant Methods | 2017 | 363 |
| 10 | Plant diseases and pests detection based on deep learning: a review | Plant Methods | 2021 | 340 |
| 11 | Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species | Plant Methods | 2014 | 339 |
| 12 | A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts | Plant Methods | 2006 | 320 |
| 13 | Plant phenotyping: from bean weighing to image analysis | Plant Methods | 2015 | 307 |
| 14 | Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry | Plant Methods | 2011 | 303 |
| 15 | Isolation of a strong Arabidopsis guard cell promoter and its potential as a research tool | Plant Methods | 2008 | 295 |
| 16 | Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize | Plant Methods | 2017 | 280 |
| 17 | Hyperspectral imaging for small-scale analysis of symptoms caused by different sugar beet diseases | Plant Methods | 2012 | 265 |
| 18 | Modeling maize above-ground biomass based on machine learning approaches using UAV remote-sensing data | Plant Methods | 2019 | 250 |
| 19 | Unmanned aerial platform-based multi-spectral imaging for field phenotyping of maize | Plant Methods | 2015 | 248 |
| 20 | Accurate inference of shoot biomass from high-throughput images of cereal plants | Plant Methods | 2011 | 243 |
| 21 | Advances in plant gene-targeted and functional markers: a review | Plant Methods | 2013 | 238 |
| 22 | Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells | Plant Methods | 2008 | 224 |
| 23 | The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species | Plant Methods | 2009 | 223 |
| 24 | A rapid, non-invasive procedure for quantitative assessment of drought survival using chlorophyll fluorescence | Plant Methods | 2008 | 215 |
| 25 | A rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation | Plant Methods | 2006 | 214 |
| 26 | Novel scanning procedure enabling the vectorization of entire rhizotron-grown root systems | Plant Methods | 2013 | 214 |
| 27 | Automated phenotyping of plant shoots using imaging methods for analysis of plant stress responses – a review | Plant Methods | 2015 | 214 |
| 28 | A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress | Plant Methods | 2008 | 209 |
| 29 | Direct comparison of MRI and X-ray CT technologies for 3D imaging of root systems in soil: potential and challenges for root trait quantification | Plant Methods | 2015 | 209 |
| 30 | AI-powered banana diseases and pest detection | Plant Methods | 2019 | 204 |
| 31 | Plant disease identification using explainable 3D deep learning on hyperspectral images | Plant Methods | 2019 | 202 |
| 32 | Application of unmanned aerial systems for high throughput phenotyping of large wheat breeding nurseries | Plant Methods | 2016 | 200 |
| 33 | A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction | Plant Methods | 2012 | 199 |
| 34 | Large scale genome skimming from herbarium material for accurate plant identification and phylogenomics | Plant Methods | 2020 | 197 |
| 35 | A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors | Plant Methods | 2007 | 196 |
| 36 | An improved allele-specific PCR primer design method for SNP marker analysis and its application | Plant Methods | 2012 | 192 |
| 37 | Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples | Plant Methods | 2014 | 188 |
| 38 | The use of plant models in deep learning: an application to leaf counting in rosette plants | Plant Methods | 2018 | 185 |
| 39 | A strategy to characterize chlorophyll protein interaction in LIL3 | Plant Methods | 2019 | 183 |
| 40 | HyperART: non-invasive quantification of leaf traits using hyperspectral absorption-reflectance-transmittance imaging | Plant Methods | 2015 | 180 |
| 41 | Protocol: Streamlined sub-protocols for floral-dip transformation and selection of transformants in Arabidopsis thaliana | Plant Methods | 2009 | 175 |
| 42 | An online database for plant image analysis software tools | Plant Methods | 2013 | 175 |
| 43 | A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction | Plant Methods | 2012 | 174 |
| 44 | Detection and analysis of wheat spikes using Convolutional Neural Networks | Plant Methods | 2018 | 173 |
| 45 | Protocol: optimising hydroponic growth systems for nutritional and physiological analysis of Arabidopsis thaliana and other plants | Plant Methods | 2013 | 167 |
| 46 | Estimating rice chlorophyll content and leaf nitrogen concentration with a digital still color camera under natural light | Plant Methods | 2014 | 167 |
| 47 | High-throughput Agrobacterium-mediated barley transformation | Plant Methods | 2008 | 163 |
| 48 | Convergence of developmental mutants into a single tomato model system: 'Micro-Tom' as an effective toolkit for plant development research | Plant Methods | 2011 | 161 |
| 49 | TasselNet: counting maize tassels in the wild via local counts regression network | Plant Methods | 2017 | 161 |
| 50 | High quality metabolomic data for Chlamydomonas reinhardtii | Plant Methods | 2008 | 160 |
