# | Title | Journal | Year | Citations |
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1 | Illumina Sequencing Library Preparation for Highly Multiplexed Target Capture and Sequencing | Cold Spring Harbor Protocols | 2010 | 1,690 |
2 | Hematoxylin and Eosin Staining of Tissue and Cell Sections | Cold Spring Harbor Protocols | 2008 | 1,225 |
3 | Purification of RNA Using TRIzol (TRI Reagent) | Cold Spring Harbor Protocols | 2010 | 1,085 |
4 | Bone Marrow-Derived Macrophages (BMM): Isolation and Applications | Cold Spring Harbor Protocols | 2008 | 677 |
5 | Purification of Nucleic Acids by Extraction with Phenol:Chloroform | Cold Spring Harbor Protocols | 2006 | 601 |
6 | Crystal Violet Assay for Determining Viability of Cultured Cells | Cold Spring Harbor Protocols | 2016 | 549 |
7 | RNA Sequencing and Analysis | Cold Spring Harbor Protocols | 2015 | 538 |
8 | DNase-seq: A High-Resolution Technique for Mapping Active Gene Regulatory Elements across the Genome from Mammalian Cells | Cold Spring Harbor Protocols | 2010 | 497 |
9 | Manual Hematoxylin and Eosin Staining of Mouse Tissue Sections | Cold Spring Harbor Protocols | 2014 | 490 |
10 | Analysis of Cell Viability by the MTT Assay | Cold Spring Harbor Protocols | 2018 | 474 |
11 | Using the Basic Local Alignment Search Tool (BLAST) | Cold Spring Harbor Protocols | 2007 | 426 |
12 | High-Throughput Illumina Strand-Specific RNA Sequencing Library Preparation | Cold Spring Harbor Protocols | 2011 | 382 |
13 | Acridine Orange/Ethidium Bromide (AO/EB) Staining to Detect Apoptosis | Cold Spring Harbor Protocols | 2006 | 313 |
14 | Using the Metagenomics RAST Server (MG-RAST) for Analyzing Shotgun Metagenomes | Cold Spring Harbor Protocols | 2010 | 291 |
15 | IMGT/V-QUEST: IMGT Standardized Analysis of the Immunoglobulin (IG) and T Cell Receptor (TR) Nucleotide Sequences | Cold Spring Harbor Protocols | 2011 | 291 |
16 | Haploview: Visualization and Analysis of SNP Genotype Data | Cold Spring Harbor Protocols | 2009 | 290 |
17 | Cetyltrimethyl Ammonium Bromide (CTAB) DNA Miniprep for Plant DNA Isolation | Cold Spring Harbor Protocols | 2009 | 277 |
18 | Strategies for Isolation of Exosomes | Cold Spring Harbor Protocols | 2015 | 276 |
19 | Quantitation of Apoptosis and Necrosis by Annexin V Binding, Propidium Iodide Uptake, and Flow Cytometry | Cold Spring Harbor Protocols | 2016 | 248 |
20 | Analysis of Cell Viability by the Lactate Dehydrogenase Assay | Cold Spring Harbor Protocols | 2018 | 235 |
21 | Transformation of Agrobacterium Using the Freeze-Thaw Method | Cold Spring Harbor Protocols | 2006 | 219 |
22 | Fluorescence Microscopy | Cold Spring Harbor Protocols | 2014 | 216 |
23 | Chromatin Immunoprecipitation (ChIP) | Cold Spring Harbor Protocols | 2009 | 203 |
24 | In Planta Visualization of Protein Interactions Using Bimolecular Fluorescence Complementation (BiFC) | Cold Spring Harbor Protocols | 2008 | 193 |
25 | Design and Construction of 2A Peptide-Linked Multicistronic Vectors | Cold Spring Harbor Protocols | 2012 | 188 |
26 | Analyzing Cell Death by Nuclear Staining with Hoechst 33342 | Cold Spring Harbor Protocols | 2016 | 186 |
27 | Isolation of High-Molecular-Weight DNA Using Organic Solvents | Cold Spring Harbor Protocols | 2017 | 170 |
28 | Bradford Assay for Determining Protein Concentration | Cold Spring Harbor Protocols | 2020 | 166 |
29 | Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry | Cold Spring Harbor Protocols | 2016 | 165 |
30 | Locomotor Activity Level Monitoring Using the Drosophila Activity Monitoring (DAM) System: Figure 1. | Cold Spring Harbor Protocols | 2010 | 160 |
31 | Human Cancer Growth and Therapy in Immunodeficient Mouse Models | Cold Spring Harbor Protocols | 2014 | 156 |
32 | Detecting Cleaved Caspase-3 in Apoptotic Cells by Flow Cytometry | Cold Spring Harbor Protocols | 2016 | 152 |
33 | The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro | Cold Spring Harbor Protocols | 2009 | 144 |
34 | Introduction to Genetic Association Studies | Cold Spring Harbor Protocols | 2012 | 140 |
35 | Imaging Neuronal Activity with Genetically Encoded Calcium Indicators | Cold Spring Harbor Protocols | 2012 | 136 |
36 | Labeling Mitochondria with MitoTracker Dyes | Cold Spring Harbor Protocols | 2011 | 135 |
37 | Labeling Nuclear DNA Using DAPI | Cold Spring Harbor Protocols | 2011 | 130 |
38 | IMGT, the International ImMunoGeneTics Information System | Cold Spring Harbor Protocols | 2011 | 130 |
39 | Tomato (Solanum lycopersicum): A Model Fruit-Bearing Crop | Cold Spring Harbor Protocols | 2008 | 127 |
40 | Growth and the Environment of Schizosaccharomyces pombe | Cold Spring Harbor Protocols | 2016 | 126 |
41 | The Parasitoid Wasp Nasonia: An Emerging Model System with Haploid Male Genetics | Cold Spring Harbor Protocols | 2009 | 120 |
42 | The Red Flour Beetle, Tribolium castaneum (Coleoptera): A Model for Studies of Development and Pest Biology: Figure 1. | Cold Spring Harbor Protocols | 2009 | 119 |
43 | Purification of Oligodendrocyte Lineage Cells from Mouse Cortices by Immunopanning | Cold Spring Harbor Protocols | 2013 | 115 |
44 | Measuring Mitochondrial Transmembrane Potential by TMRE Staining | Cold Spring Harbor Protocols | 2016 | 102 |
45 | Adhesive Micropatterns for Cells: A Microcontact Printing Protocol | Cold Spring Harbor Protocols | 2009 | 101 |
46 | Maize (Zea mays): A Model Organism for Basic and Applied Research in Plant Biology | Cold Spring Harbor Protocols | 2009 | 99 |
47 | Precipitation of DNA with Ethanol | Cold Spring Harbor Protocols | 2016 | 99 |
48 | Ca2+-Sensitive Fluorescent Dyes and Intracellular Ca2+ Imaging | Cold Spring Harbor Protocols | 2013 | 98 |
49 | Analysis of Glial Cell Development and Function in Drosophila | Cold Spring Harbor Protocols | 2012 | 97 |
50 | Fluorescence Perturbation Techniques to Study Mobility and Molecular Dynamics of Proteins in Live Cells: FRAP, Photoactivation, Photoconversion, and FLIP | Cold Spring Harbor Protocols | 2010 | 94 |