2.5(top 7%)
Impact Factor
2.8(top 7%)
extended IF
161(top 2%)
H-Index
1.7K
authors
6.9K
papers
185K
citations
6.4K
citing journals
90.9K
citing authors

Most Cited Articles of BioTechniques

TitleYearCitations
TM4: a free, open-source system for microarray data management and analysis20034K
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material19913.5K
A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples19931.7K
Improved retroviral vectors for gene transfer and expression19891.4K
ImageJ for microscopy20071.3K
Real-time PCR for mRNA quantitation20051.2K
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking20041.2K
Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping19961K
Improved extraction of PCR-quality community DNA from digesta and fecal samples2004990
Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT)2000978
Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction1990972
The "megaprimer" method of site-directed mutagenesis1990953
Continuous fluorescence monitoring of rapid cycle DNA amplification1997951
Processing of gene expression data generated by quantitative real-time RT-PCR2002901
The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences2000895
The LightCycler: a microvolume multisample fluorimeter with rapid temperature control1997802
Luciferase reporter gene vectors for analysis of promoters and enhancers1988802
Quantitative RT-PCR: pitfalls and potential1999792
Twenty-five years of quantitative PCR for gene expression analysis2008790
A concise guide to cDNA microarray analysis2000766
Mitochondrial membrane potential probes and the proton gradient: a practical usage guide2011732
Validation of housekeeping genes for normalizing RNA expression in real-time PCR2004729
Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA1988724
Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites1999669
pBBR1MCS: a broad-host-range cloning vector1994662