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BioTechniques
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Top Articles
BioTechniques
Biology
,
Biotechnology
2.5
(top 7%)
Impact Factor
2.8
(top 7%)
extended IF
161
(top 2%)
H-Index
1.7K
authors
6.9K
papers
185K
citations
6.4K
citing journals
90.9K
citing authors
Most Cited Articles of BioTechniques
Title
Year
Citations
TM4: a free, open-source system for microarray data management and analysis
2003
4K
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material
1991
3.5K
A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples
1993
1.7K
Improved retroviral vectors for gene transfer and expression
1989
1.4K
ImageJ for microscopy
2007
1.3K
Real-time PCR for mRNA quantitation
2005
1.2K
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking
2004
1.2K
Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping
1996
1K
Improved extraction of PCR-quality community DNA from digesta and fecal samples
2004
990
Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT)
2000
978
Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction
1990
972
The "megaprimer" method of site-directed mutagenesis
1990
953
Continuous fluorescence monitoring of rapid cycle DNA amplification
1997
951
Processing of gene expression data generated by quantitative real-time RT-PCR
2002
901
The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences
2000
895
The LightCycler: a microvolume multisample fluorimeter with rapid temperature control
1997
802
Luciferase reporter gene vectors for analysis of promoters and enhancers
1988
802
Quantitative RT-PCR: pitfalls and potential
1999
792
Twenty-five years of quantitative PCR for gene expression analysis
2008
790
A concise guide to cDNA microarray analysis
2000
766
Mitochondrial membrane potential probes and the proton gradient: a practical usage guide
2011
732
Validation of housekeeping genes for normalizing RNA expression in real-time PCR
2004
729
Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA
1988
724
Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites
1999
669
pBBR1MCS: a broad-host-range cloning vector
1994
662
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How are inpact factors calculated?
The impact factor (IF) is calculated by counting citations from peer-reviewed journals only.
extended IF
also counts citations from books and conference papers. However, no patent, abstract, working papers, online documents, etc., are covered.
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