| # | Title | Journal | Year | Citations |
|---|
| 1 | TM4: A Free, Open-Source System for Microarray Data Management and Analysis | BioTechniques | 2003 | 4,443 |
| 2 | Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material | BioTechniques | 1991 | 3,740 |
| 3 | A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples | BioTechniques | 1993 | 1,857 |
| 4 | ImageJ for microscopy | BioTechniques | 2007 | 1,684 |
| 5 | Improved retroviral vectors for gene transfer and expression | BioTechniques | 1989 | 1,630 |
| 6 | Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material | BioTechniques | 2013 | 1,624 |
| 7 | Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking | BioTechniques | 2004 | 1,428 |
| 8 | Real-time PCR for mRNA quantitation | BioTechniques | 2005 | 1,411 |
| 9 | Improved extraction of PCR-quality community DNA from digesta and fecal samples | BioTechniques | 2004 | 1,342 |
| 10 | The Sequence Manipulation Suite: JavaScript Programs for Analyzing and Formatting Protein and DNA Sequences | BioTechniques | 2000 | 1,306 |
| 11 | Preparation of PCR-Quality Mouse Genomic DNA with Hot Sodium Hydroxide and Tris (HotSHOT) | BioTechniques | 2000 | 1,290 |
| 12 | Modulation of Non-Templated Nucleotide Addition by Taq DNA Polymerase: Primer Modifications that Facilitate Genotyping | BioTechniques | 1996 | 1,137 |
| 13 | Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction | BioTechniques | 1990 | 1,047 |
| 14 | Continuous Fluorescence Monitoring of Rapid Cycle DNA Amplification | BioTechniques | 1997 | 1,017 |
| 15 | The "megaprimer" method of site-directed mutagenesis | BioTechniques | 1990 | 1,010 |
| 16 | Processing of gene expression data generated by quantitative real-time RT-PCR | BioTechniques | 2002 | 964 |
| 17 | Twenty-five years of quantitative PCR for gene expression analysis | BioTechniques | 2008 | 961 |
| 18 | Quantitative RT-PCR: Pitfalls and Potential | BioTechniques | 1999 | 924 |
| 19 | Mitochondrial membrane potential probes and the proton gradient: a practical usage guide | BioTechniques | 2011 | 924 |
| 20 | The LightCyclerTM: A Microvolume Multisample Fluorimeter with Rapid Temperature Control | BioTechniques | 1997 | 877 |
| 21 | A Concise Guide to cDNA Microarray Analysis | BioTechniques | 2000 | 846 |
| 22 | Luciferase reporter gene vectors for analysis of promoters and enhancers | BioTechniques | 1988 | 842 |
| 23 | Validation of housekeeping genes for normalizing RNA expression in real-time PCR | BioTechniques | 2004 | 838 |
| 24 | Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue | BioTechniques | 2009 | 820 |
| 25 | CellProfiler™: free, versatile software for automated biological image analysis | BioTechniques | 2007 | 801 |
| 26 | pBBR1MCS: a broad-host-range cloning vector | BioTechniques | 1994 | 767 |
| 27 | Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA | BioTechniques | 1988 | 764 |
| 28 | Multiplex PCR: Critical Parameters and Step-by-Step Protocol | BioTechniques | 1997 | 739 |
| 29 | Control Selection for RNA Quantitation | BioTechniques | 2000 | 730 |
| 30 | Universal Linker and Ligation Procedures for Construction of Genomic DNA Libraries Enriched for Microsatellites | BioTechniques | 1999 | 705 |
| 31 | Two-step Red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli | BioTechniques | 2006 | 703 |
| 32 | Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology | BioTechniques | 1991 | 702 |
| 33 | High-Content Screening | BioTechniques | 2007 | 686 |
| 34 | Facile means for quantifying microRNA expression by real-time PCR | BioTechniques | 2005 | 663 |
| 35 | Reverse Transcriptase Template Switching: A SMART™ Approach for Full-Length cDNA Library Construction | BioTechniques | 2001 | 661 |
| 36 | A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications | BioTechniques | 1993 | 653 |
| 37 | Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification | BioTechniques | 1998 | 599 |
| 38 | PCR Fusion-Based Approach to Create Reporter Gene Constructs for Expression Analysis in Transgenic C. elegans | BioTechniques | 2002 | 589 |
| 39 | Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChangeTM Site-Directed Mutagenesis | BioTechniques | 1999 | 566 |
| 40 | A simple and efficient procedure for transformation of yeasts | BioTechniques | 1992 | 558 |
| 41 | Microsatellite Libraries Enriched for Several Microsatellite Sequences in Plants | BioTechniques | 1996 | 556 |
| 42 | Antibody validation | BioTechniques | 2010 | 548 |
| 43 | Rapid silver staining and recovery of PCR products separated on polyacrylamide gels | BioTechniques | 1994 | 538 |
| 44 | "Checkerboard" DNA-DNA hybridization | BioTechniques | 1994 | 537 |
| 45 | TScratch: a novel and simple software tool for automated analysis of monolayer wound healing assays | BioTechniques | 2009 | 532 |
| 46 | M13-Tailed Primers Improve the Readability and Usability of Microsatellite Analyses Performed with Two Different Allele- Sizing Methods | BioTechniques | 2001 | 527 |
| 47 | Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase | BioTechniques | 1988 | 516 |
| 48 | High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences | BioTechniques | 2007 | 514 |
| 49 | Standardized quantification of pulmonary fibrosis in histological samples | BioTechniques | 2008 | 503 |
| 50 | An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction | BioTechniques | 1990 | 500 |
