| # | Title | Journal | Year | Citations |
|---|
| 1 | Protein production by auto-induction in high-density shaking cultures | Protein Expression and Purification | 2005 | 4,981 |
| 2 | Purification of brain tubulin through two cycles of polymerization–depolymerization in a high-molarity buffer | Protein Expression and Purification | 2003 | 639 |
| 3 | Overcoming Expression and Purification Problems of RhoGDI Using a Family of “Parallel” Expression Vectors | Protein Expression and Purification | 1999 | 606 |
| 4 | Immobilized metal ion affinity chromatography | Protein Expression and Purification | 1992 | 554 |
| 5 | Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins | Protein Expression and Purification | 2006 | 543 |
| 6 | A New Vector for High-Throughput, Ligation-Independent Cloning Encoding a Tobacco Etch Virus Protease Cleavage Site | Protein Expression and Purification | 2002 | 489 |
| 7 | SUMO fusion technology for difficult-to-express proteins | Protein Expression and Purification | 2005 | 379 |
| 8 | Practical considerations in refolding proteins from inclusion bodies | Protein Expression and Purification | 2003 | 366 |
| 9 | Comparison of affinity tags for protein purification | Protein Expression and Purification | 2005 | 356 |
| 10 | Affinity Fusion Strategies for Detection, Purification, and Immobilization of Recombinant Proteins | Protein Expression and Purification | 1997 | 302 |
| 11 | Three Phase Partitioning: Concentration and Purification of Proteins | Protein Expression and Purification | 1997 | 280 |
| 12 | Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study | Protein Expression and Purification | 2008 | 273 |
| 13 | An overview of enzymatic reagents for the removal of affinity tags | Protein Expression and Purification | 2011 | 268 |
| 14 | Purification and characterization of C-Phycocyanin from cyanobacterial species of marine and freshwater habitat | Protein Expression and Purification | 2005 | 251 |
| 15 | Recombinant production of antimicrobial peptides in Escherichia coli: A review | Protein Expression and Purification | 2011 | 241 |
| 16 | A combined approach to improving large-scale production of tobacco etch virus protease | Protein Expression and Purification | 2007 | 240 |
| 17 | High-Level Expression of Soluble Protein inEscherichia coliUsing a His6-Tag and Maltose-Binding-Protein Double-Affinity Fusion System | Protein Expression and Purification | 1997 | 224 |
| 18 | Roles of NADPH-P450 Reductase and Apo- and Holo-Cytochrome b5 on Xenobiotic Oxidations Catalyzed by 12 Recombinant Human Cytochrome P450s Expressed in Membranes of Escherichia coli | Protein Expression and Purification | 2002 | 224 |
| 19 | One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag | Protein Expression and Purification | 2001 | 221 |
| 20 | Purification of His-Tagged Proteins by Immobilized Chelate Affinity Chromatography: The Benefits from the Use of Organic Solvent | Protein Expression and Purification | 2000 | 217 |
| 21 | Product Purification by Reversible Phase Transition FollowingEscherichia coliExpression of Genes Encoding up to 251 Repeats of the Elastomeric Pentapeptide GVGVP | Protein Expression and Purification | 1996 | 216 |
| 22 | A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa | Protein Expression and Purification | 2003 | 214 |
| 23 | Optimization of Inclusion Body Solubilization and Renaturation of Recombinant Human Growth Hormone from Escherichia coli | Protein Expression and Purification | 2000 | 203 |
| 24 | Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3 | Protein Expression and Purification | 2005 | 200 |
| 25 | A Modular Polycistronic Expression System for Overexpressing Protein Complexes in Escherichia coli | Protein Expression and Purification | 2001 | 199 |
| 26 | Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost | Protein Expression and Purification | 2007 | 190 |
| 27 | Elution of antibodies from a Protein-A column by aqueous arginine solutions | Protein Expression and Purification | 2004 | 181 |
| 28 | The toxicity of recombinant proteins in Escherichia coli: a comparison of overexpression in BL21(DE3), C41(DE3), and C43(DE3) | Protein Expression and Purification | 2004 | 180 |
| 29 | Optimization of protein purification and characterization using Thermofluor screens | Protein Expression and Purification | 2013 | 178 |
| 30 | Controlled Intracellular Processing of Fusion Proteins by TEV Protease | Protein Expression and Purification | 2000 | 177 |
| 31 | Production of Correctly Folded Fab Antibody Fragment in the Cytoplasm of Escherichia coli trxB gor Mutants via the Coexpression of Molecular Chaperones | Protein Expression and Purification | 2001 | 172 |
| 32 | Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors | Protein Expression and Purification | 2005 | 170 |
| 33 | From gene to protein: a review of new and enabling technologies for multi-parallel protein expression | Protein Expression and Purification | 2005 | 169 |
| 34 | In vitro protein refolding by chromatographic procedures | Protein Expression and Purification | 2004 | 168 |
| 35 | PA tag: A versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin | Protein Expression and Purification | 2014 | 168 |
| 36 | Low-Temperature Increases the Yield of Biologically Active Herring Antifreeze Protein in Pichia pastoris | Protein Expression and Purification | 2001 | 165 |
| 37 | High-Throughput Proteomics: Protein Expression and Purification in the Postgenomic World | Protein Expression and Purification | 2001 | 165 |
| 38 | A new method for purification of recombinant human α-synuclein in Escherichia coli | Protein Expression and Purification | 2005 | 163 |
| 39 | Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants | Protein Expression and Purification | 2013 | 163 |
| 40 | An expression vector tailored for large-scale, high-throughput purification of recombinant proteins | Protein Expression and Purification | 2006 | 161 |
| 41 | Production of disulfide-bonded proteins in Escherichia coli | Protein Expression and Purification | 2012 | 161 |
| 42 | Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis | Protein Expression and Purification | 2004 | 159 |
| 43 | The pST44 polycistronic expression system for producing protein complexes in Escherichia coli | Protein Expression and Purification | 2005 | 159 |
| 44 | Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners | Protein Expression and Purification | 2006 | 159 |
| 45 | Expression and Purification of a Spider Silk Protein: A New Strategy for Producing Repetitive Proteins | Protein Expression and Purification | 1996 | 157 |
| 46 | Isolation, structural characterization, and antiviral activity of positional isomers of monopegylated interferon α-2a (PEGASYS) | Protein Expression and Purification | 2003 | 155 |
| 47 | pJC20 and pJC40 - Two High-Copy-Number Vectors for T7 RNA Polymerase-Dependent Expression of Recombinant Genes in Escherichia coli | Protein Expression and Purification | 1994 | 151 |
| 48 | Expression of Aggregation-Prone Recombinant Proteins at Low Temperatures: A Comparative Study of theEscherichia coli cspAandtacPromoter Systems | Protein Expression and Purification | 1997 | 148 |
| 49 | Expression in Trichoderma reesei and characterisation of a thermostable family 3 β-glucosidase from the moderately thermophilic fungus Talaromyces emersonii | Protein Expression and Purification | 2004 | 146 |
| 50 | Single step protocol to purify recombinant proteins with low endotoxin contents | Protein Expression and Purification | 2006 | 146 |
