3.8(top 4%)
Impact Factor
4(top 4%)
extended IF
262(top 1%)
H-Index
12.2K
authors
23.6K
papers
815.8K
citations
10.4K
citing journals
224.3K
citing authors

Most Cited Articles of Gene

TitleYearCitations
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors198514.9K
Site-directed mutagenesis by overlap extension using the polymerase chain reaction19897.3K
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers19826.1K
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase19885.7K
Construction and characterization of new cloning vehicle. II. A multipurpose cloning system19775.1K
A simple and very efficient method for generating cDNA libraries19834.6K
Efficient selection for high-expression transfectants with a novel eukaryotic vector19914.5K
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing19844.2K
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer19883K
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments19823K
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension19892.7K
New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites19882.7K
Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes19952.7K
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system19772.6K
FACS-optimized mutants of the green fluorescent protein (GFP)19962.5K
Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis19832.4K
A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli19872.3K
The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions19882.2K
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum19942.1K
Primary structure of the Aequorea victoria green-fluorescent protein19921.8K
In vitro insertional mutagenesis with a selectable DNA fragment19841.6K
High efficiency transformation of Escherichia coli with plasmids19901.6K
Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds19951.6K
Improved single and multicopy lac-based cloning vectors for protein and operon fusions19871.5K
The 'effective number of codons' used in a gene19901.5K