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Top Articles

#TitleJournalYearCitations
1Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene198516,642
2Site-directed mutagenesis by overlap extension using the polymerase chain reactionGene19897,982
3The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene19826,767
4Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene19886,320
5Construction and characterization of new cloning vehicle. II. A multipurpose cloning systemGene19775,197
6Efficient selection for high-expression transfectants with a novel eukaryotic vectorGene19914,955
7A simple and very efficient method for generating cDNA librariesGene19834,907
8Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencingGene19844,620
9CLUSTAL: a package for performing multiple sequence alignment on a microcomputerGene19883,467
10A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragmentsGene19823,300
11Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettesGene19953,154
12Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extensionGene19893,004
13New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sitesGene19882,939
14FACS-optimized mutants of the green fluorescent protein (GFP)Gene19962,862
15Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesisGene19832,698
16The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regionsGene19882,672
17A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coliGene19872,631
18Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicumGene19942,595
19Primary structure of the Aequorea victoria green-fluorescent proteinGene19921,993
20The ‘effective number of codons’ used in a geneGene19901,980
21High efficiency transformation of Escherichia coli with plasmidsGene19901,884
22Yeast vectors for the controlled expression of heterologous proteins in different genetic backgroundsGene19951,827
23A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutantsGene19981,819
24In vitro insertional mutagenesis with a selectable DNA fragmentGene19841,781
25Epidermal growth factor receptor (EGFR) signaling in cancerGene20061,781
26Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinantGene19951,745
27Multifunctional yeast high-copy-number shuttle vectorsGene19921,675
28Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegansGene20011,643
29Improved single and multicopy lac-based cloning vectors for protein and operon fusionsGene19871,622
30Vectors for selective expression of cloned DNAs by T7 RNA polymeraseGene19871,599
31Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteriaGene19881,560
32Prolonged incubation in calcium chloride improves the competence of Escherichia coli cellsGene19791,540
33Acetylation and deacetylation of non-histone proteinsGene20051,495
34Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.Gene19921,320
35A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vectorGene19871,277
36Genetics and biology of vitamin D receptor polymorphismsGene20041,276
37Initiation of translation in prokaryotes and eukaryotesGene19991,272
38Plasmid screening at high colony densityGene19801,213
39Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 geneGene19791,211
40Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in PseudomonasGene19811,195
41The essence of SNPsGene19991,177
42Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coliGene19911,114
43Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vectorGene19861,096
44Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experimentsGene19791,087
45A simple phase-extraction assay for chloramphenicol acyltransferase activityGene19881,069
46Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coliGene19881,051
47Thiamine-repressible expression vectors pREP and pRIP for fission yeastGene19931,041
48Construction and characterization of new cloning vehicles I. Ampicillin-resistant derivatives of the plasmid pMB9Gene19771,036
49The Sp-family of transcription factorsGene19991,030
50Function of alternative splicingGene20051,030