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Top Articles

#TitleJournalYearCitations
1[20] Processing of X-ray diffraction data collected in oscillation modeMethods in Enzymology199730,205
2[13] Catalase in vitroMethods in Enzymology198419,026
3[57] Sequencing end-labeled DNA with base-specific chemical cleavagesMethods in Enzymology198015,323
4Processing of X-ray diffraction data collected in oscillation modeMethods in Enzymology199712,685
5[14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagentMethods in Enzymology199911,559
6[30] Microsomal lipid peroxidationMethods in Enzymology19789,907
7[34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranesMethods in Enzymology19878,982
8[6] Use of T7 RNA polymerase to direct expression of cloned genesMethods in Enzymology19906,505
9[19] Rapid and efficient site-specific mutagenesis without phenotypic selectionMethods in Enzymology19875,691
10[2] New M13 vectors for cloningMethods in Enzymology19835,560
11[49] Determination of carbonyl content in oxidatively modified proteinsMethods in Enzymology19904,778
12[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reactionMethods in Enzymology19874,551
13[1] Role of free radicals and catalytic metal ions in human disease: An overviewMethods in Enzymology19903,941
14[12] Assays of glutathione peroxidaseMethods in Enzymology19843,904
15[26] Raster3D: Photorealistic molecular graphicsMethods in Enzymology19973,518
16[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombeMethods in Enzymology19913,505
17[12] One-step gene disruption in yeastMethods in Enzymology19833,452
18[136] Assay of catalases and peroxidasesMethods in Enzymology19553,446
19[17] Amylases, α and βMethods in Enzymology19553,307
20[43] Malondialdehyde determination as index of lipid PeroxidationMethods in Enzymology19903,239
21[10] Assay of inorganic phosphate, total phosphate and phosphatasesMethods in Enzymology19662,734
22[1] Production of single-stranded plasmid DNAMethods in Enzymology19872,717
23[2] Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentrationMethods in Enzymology19992,682
24[42] Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenalMethods in Enzymology19902,680
25[1] Getting started with yeastMethods in Enzymology19912,668
26[73] Spectrophotometric and turbidimetric methods for measuring proteinsMethods in Enzymology19572,608
27Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol methodMethods in Enzymology20022,406
28[59] Glutathione reductaseMethods in Enzymology19852,332
29[16] SHELXL: High-resolution refinementMethods in Enzymology19972,318
30[70] Determination of glutathione and glutathione disulfide in biological samplesMethods in Enzymology19852,308
31[37] Carbonyl assays for determination of oxidatively modified proteinsMethods in Enzymology19942,211
32[14]Determination and analysis of urea and guanidine hydrochloride denaturation curvesMethods in Enzymology19862,070
33[38] Oxidative damage to proteins: Spectrophotometric method for carbonyl assayMethods in Enzymology19941,978
34[51] Assays for differentiation of glutathione S-TransferasesMethods in Enzymology19811,928
35Getting started with yeastMethods in Enzymology20021,896
36[20] VERIFY3D: Assessment of protein models with three-dimensional profilesMethods in Enzymology19971,839
37[1] Citrate synthaseMethods in Enzymology19691,812
38[6] Statistical analysis of enzyme kinetic dataMethods in Enzymology19791,776
39[5] Rapid and sensitive sequence comparison with FASTP and FASTAMethods in Enzymology19901,771
40[9] TM4 Microarray Software SuiteMethods in Enzymology20061,767
41[27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methodsMethods in Enzymology19971,761
42[1] Measurement of molecular weights by electrophoresis on SDS-acrylamide gelMethods in Enzymology19721,651
43[69] Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membraneMethods in Enzymology19741,651
44Rosetta3Methods in Enzymology20111,620
45[1] Preparation of monoclonal antibodies: Strategies and proceduresMethods in Enzymology19811,568
46[19] Receptor-mediated endocytosis of low-density lipoprotein in cultured cellsMethods in Enzymology19831,557
47[11] Calculation of protein conformation from circular dichroismMethods in Enzymology19861,539
48[41] Cathepsin B, cathepsin H, and cathepsin LMethods in Enzymology19811,533
49[68] Transcription maps of polyoma virus-specific RNA: Analysis by two-dimensional nuclease S1 gel mappingMethods in Enzymology19801,503
50[11] Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose platesMethods in Enzymology19911,485