| # | Title | Journal | Year | Citations |
|---|
| 1 | The effects of alternate optimal solutions in constraint-based genome-scale metabolic models | Metabolic Engineering | 2003 | 1,083 |
| 2 | Metabolic engineering of Escherichia coli for 1-butanol production | Metabolic Engineering | 2008 | 764 |
| 3 | 13C Metabolic Flux Analysis | Metabolic Engineering | 2001 | 725 |
| 4 | Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production | Metabolic Engineering | 2013 | 573 |
| 5 | Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism | Metabolic Engineering | 2010 | 537 |
| 6 | From zero to hero—Design-based systems metabolic engineering of Corynebacterium glutamicum for l-lysine production | Metabolic Engineering | 2011 | 528 |
| 7 | Metabolic Fluxes and Metabolic Engineering | Metabolic Engineering | 1999 | 522 |
| 8 | Elementary metabolite units (EMU): A novel framework for modeling isotopic distributions | Metabolic Engineering | 2007 | 514 |
| 9 | A comparative view of metabolite and substrate stress and tolerance in microbial bioprocessing: From biofuels and chemicals, to biocatalysis and bioremediation | Metabolic Engineering | 2010 | 478 |
| 10 | Biorefineries for the production of top building block chemicals and their derivatives | Metabolic Engineering | 2015 | 425 |
| 11 | Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements | Metabolic Engineering | 2006 | 423 |
| 12 | Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli | Metabolic Engineering | 2005 | 422 |
| 13 | Microbial export of lactic and 3-hydroxypropanoic acid: implications for industrial fermentation processes | Metabolic Engineering | 2004 | 409 |
| 14 | Global transcription machinery engineering: A new approach for improving cellular phenotype | Metabolic Engineering | 2007 | 398 |
| 15 | Metabolic Engineering of Fatty Acid Biosynthesis in Plants | Metabolic Engineering | 2002 | 392 |
| 16 | Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli | Metabolic Engineering | 2007 | 388 |
| 17 | Cell-free synthetic biology: Thinking outside the cell | Metabolic Engineering | 2012 | 365 |
| 18 | Synthetic biology and the development of tools for metabolic engineering | Metabolic Engineering | 2012 | 363 |
| 19 | Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae | Metabolic Engineering | 2015 | 355 |
| 20 | Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide | Metabolic Engineering | 2011 | 352 |
| 21 | Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing | Metabolic Engineering | 2015 | 351 |
| 22 | Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards Taxol (Paclitaxel) production | Metabolic Engineering | 2008 | 350 |
| 23 | Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways | Metabolic Engineering | 2008 | 350 |
| 24 | Engineering alternative butanol production platforms in heterologous bacteria | Metabolic Engineering | 2009 | 350 |
| 25 | Metabolic Engineering of Osmoprotectant Accumulation in Plants | Metabolic Engineering | 2002 | 349 |
| 26 | A Universal Framework for 13C Metabolic Flux Analysis | Metabolic Engineering | 2001 | 343 |
| 27 | Selection and optimization of microbial hosts for biofuels production | Metabolic Engineering | 2008 | 343 |
| 28 | Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production | Metabolic Engineering | 2013 | 343 |
| 29 | Overproduction of free fatty acids in E. coli: Implications for biodiesel production | Metabolic Engineering | 2008 | 341 |
| 30 | Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol | Metabolic Engineering | 2010 | 338 |
| 31 | Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals | Metabolic Engineering | 2014 | 338 |
| 32 | Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism | Metabolic Engineering | 2018 | 338 |
| 33 | Deleting the para-nitrophenyl phosphatase (pNPPase), PHO13, in recombinant Saccharomyces cerevisiae improves growth and ethanol production on d-xylose | Metabolic Engineering | 2008 | 332 |
| 34 | Kinetic modelling reveals current limitations in the production of ethanol from xylose by recombinant Saccharomyces cerevisiae | Metabolic Engineering | 2011 | 316 |
| 35 | In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production | Metabolic Engineering | 2006 | 311 |
| 36 | Selective reduction of xylose to xylitol from a mixture of hemicellulosic sugars | Metabolic Engineering | 2010 | 309 |
| 37 | Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis | Metabolic Engineering | 2011 | 307 |
| 38 | The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology | Metabolic Engineering | 2019 | 307 |
| 39 | Heterologous expression of d-xylulokinase from Pichia stipitis enables high levels of xylitol production by engineered Escherichia coli growing on xylose | Metabolic Engineering | 2009 | 306 |
| 40 | Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoids | Metabolic Engineering | 2007 | 302 |
| 41 | Genome-scale metabolic network modeling results in minimal interventions that cooperatively force carbon flux towards malonyl-CoA | Metabolic Engineering | 2011 | 300 |
| 42 | Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis | Metabolic Engineering | 2011 | 296 |
| 43 | An optimization framework for identifying reaction activation/inhibition or elimination candidates for overproduction in microbial systems | Metabolic Engineering | 2006 | 292 |
| 44 | Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica | Metabolic Engineering | 2015 | 291 |
| 45 | The future of metabolic engineering and synthetic biology: Towards a systematic practice | Metabolic Engineering | 2012 | 277 |
| 46 | Optimization of a heterologous pathway for the production of flavonoids from glucose | Metabolic Engineering | 2011 | 276 |
| 47 | Metabolic engineering in methanotrophic bacteria | Metabolic Engineering | 2015 | 274 |
| 48 | Metabolic Engineering for Microbial Production of Aromatic Amino Acids and Derived Compounds | Metabolic Engineering | 2001 | 272 |
| 49 | Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism | Metabolic Engineering | 2013 | 268 |
| 50 | Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids | Metabolic Engineering | 2014 | 260 |
| 51 | Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene | Metabolic Engineering | 2009 | 259 |
| 52 | Use of modular, synthetic scaffolds for improved production of glucaric acid in engineered E. coli | Metabolic Engineering | 2010 | 258 |
| 53 | Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli | Metabolic Engineering | 2011 | 257 |
| 54 | Metabolic engineering tanshinone biosynthetic pathway in Salvia miltiorrhiza hairy root cultures | Metabolic Engineering | 2011 | 256 |
| 55 | Metabolic Engineering of Escherichia coli: Increase of NADH Availability by Overexpressing an NAD+-Dependent Formate Dehydrogenase | Metabolic Engineering | 2002 | 254 |
| 56 | Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering | Metabolic Engineering | 2008 | 254 |
| 57 | Metabolic engineering of muconic acid production in Saccharomyces cerevisiae | Metabolic Engineering | 2013 | 251 |
| 58 | Lipid engineering combined with systematic metabolic engineering of Saccharomyces cerevisiae for high-yield production of lycopene | Metabolic Engineering | 2019 | 251 |
| 59 | Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable rapid xylose utilization and ethanol production by Saccharomyces cerevisiae | Metabolic Engineering | 2012 | 250 |
| 60 | Engineering central metabolic modules of Escherichia coli for improving β-carotene production | Metabolic Engineering | 2013 | 250 |
| 61 | Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation | Metabolic Engineering | 2015 | 250 |
| 62 | Escherichia coli as a host for metabolic engineering | Metabolic Engineering | 2018 | 250 |
| 63 | The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica | Metabolic Engineering | 2015 | 249 |
| 64 | From zero to hero – Production of bio-based nylon from renewable resources using engineered Corynebacterium glutamicum | Metabolic Engineering | 2014 | 246 |
| 65 | Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform | Metabolic Engineering | 2012 | 244 |
| 66 | De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae | Metabolic Engineering | 2015 | 242 |
| 67 | Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products | Metabolic Engineering | 2008 | 240 |
| 68 | Predictive design of mRNA translation initiation region to control prokaryotic translation efficiency | Metabolic Engineering | 2013 | 240 |
| 69 | Kinetic models in industrial biotechnology – Improving cell factory performance | Metabolic Engineering | 2014 | 238 |
| 70 | Low-Copy Plasmids can Perform as Well as or Better Than High-Copy Plasmids for Metabolic Engineering of Bacteria | Metabolic Engineering | 2000 | 237 |
| 71 | Microbial acetyl-CoA metabolism and metabolic engineering | Metabolic Engineering | 2015 | 237 |
| 72 | Metabolic Engineering through Cofactor Manipulation and Its Effects on Metabolic Flux Redistribution in Escherichia coli | Metabolic Engineering | 2002 | 234 |
| 73 | Genetic Engineering of a Zeaxanthin-rich Potato by Antisense Inactivation and Co-suppression of Carotenoid Epoxidation | Metabolic Engineering | 2002 | 233 |
| 74 | Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae | Metabolic Engineering | 2007 | 233 |
| 75 | Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum | Metabolic Engineering | 2011 | 233 |
| 76 | Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production | Metabolic Engineering | 2009 | 232 |
| 77 | Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications | Metabolic Engineering | 2018 | 228 |
| 78 | Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli | Metabolic Engineering | 2006 | 227 |
| 79 | Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity | Metabolic Engineering | 2005 | 226 |
| 80 | From lignin to nylon: Cascaded chemical and biochemical conversion using metabolically engineered Pseudomonas putida | Metabolic Engineering | 2018 | 225 |
| 81 | Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria | Metabolic Engineering | 2011 | 224 |
| 82 | Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering | Metabolic Engineering | 2009 | 223 |
| 83 | Styrene biosynthesis from glucose by engineered E. coli | Metabolic Engineering | 2011 | 222 |
| 84 | Metabolic flux analysis of CHO cells at growth and non-growth phases using isotopic tracers and mass spectrometry | Metabolic Engineering | 2011 | 222 |
| 85 | Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB-co-4HB) biosynthesis | Metabolic Engineering | 2015 | 222 |
| 86 | Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio | Metabolic Engineering | 2009 | 221 |
| 87 | Model-driven evaluation of the production potential for growth-coupled products of Escherichia coli | Metabolic Engineering | 2010 | 221 |
| 88 | Isotopomer Analysis Using GC-MS | Metabolic Engineering | 1999 | 218 |
| 89 | Harnessing yeast subcellular compartments for the production of plant terpenoids | Metabolic Engineering | 2011 | 218 |
| 90 | Metabolic flux analysis in a nonstationary system: Fed-batch fermentation of a high yielding strain of E. coli producing 1,3-propanediol | Metabolic Engineering | 2007 | 217 |
| 91 | Spatial organization of enzymes for metabolic engineering | Metabolic Engineering | 2012 | 217 |
| 92 | Dynamic control of gene expression in Saccharomyces cerevisiae engineered for the production of plant sesquitepene α-santalene in a fed-batch mode | Metabolic Engineering | 2012 | 215 |
| 93 | Involvement of the G3P shuttle and β-oxidation pathway in the control of TAG synthesis and lipid accumulation in Yarrowia lipolytica | Metabolic Engineering | 2011 | 214 |
| 94 | Conversion of Xylose to Ethanol by Recombinant Saccharomyces cerevisiae: Importance of Xylulokinase (XKS1) and Oxygen Availability | Metabolic Engineering | 2001 | 213 |
| 95 | Establishment of a yeast platform strain for production of p-coumaric acid through metabolic engineering of aromatic amino acid biosynthesis | Metabolic Engineering | 2015 | 213 |
| 96 | Modular co-culture engineering, a new approach for metabolic engineering | Metabolic Engineering | 2016 | 211 |
| 97 | Experimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids | Metabolic Engineering | 2016 | 210 |
| 98 | Metabolic engineering of the complete pathway leading to heterologous biosynthesis of various flavonoids and stilbenoids in Saccharomyces cerevisiae | Metabolic Engineering | 2009 | 208 |
| 99 | Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin | Metabolic Engineering | 2015 | 205 |
| 100 | A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery | Metabolic Engineering | 2016 | 204 |
