| # | Title | Journal | Year | Citations |
|---|
| 1 | Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria | Proceedings of the National Academy of Sciences of the United States of America | 2012 | 2,217 |
| 2 | Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants | Nature Reviews Microbiology | 2020 | 1,427 |
| 3 | Single-Cell Transcriptomics of Human and Mouse Lung Cancers Reveals Conserved Myeloid Populations across Individuals and Species | Immunity | 2019 | 897 |
| 4 | A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte | Nature | 2018 | 795 |
| 5 | The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli | Nucleic Acids Research | 2011 | 701 |
| 6 | Single-cell barcoding and sequencing using droplet microfluidics | Nature Protocols | 2017 | 589 |
| 7 | Evolutionary history and global spread of the Mycobacterium tuberculosis Beijing lineage | Nature Genetics | 2015 | 466 |
| 8 | Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes | Proceedings of the National Academy of Sciences of the United States of America | 2014 | 397 |
| 9 | Origins and Evolution of the Global RNA Virome | MBio | 2018 | 383 |
| 10 | A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems | Science | 2017 | 382 |
| 11 | Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system | EMBO Journal | 2011 | 363 |
| 12 | Mycobacterium tuberculosis lineage 4 comprises globally distributed and geographically restricted sublineages | Nature Genetics | 2016 | 326 |
| 13 | Single-cell gene expression reveals a landscape of regulatory T cell phenotypes shaped by the TCR | Nature Immunology | 2018 | 312 |
| 14 | Programmable RNA Shredding by the Type III-A CRISPR-Cas System of Streptococcus thermophilus | Molecular Cell | 2014 | 278 |
| 15 | Osteoblasts remotely supply lung tumors with cancer-promoting SiglecF high neutrophils | Science | 2017 | 270 |
| 16 | Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition | Molecular Cell | 2016 | 224 |
| 17 | 5-hmC in the brain is abundant in synaptic genes and shows differences at the exon-intron boundary | Nature Structural and Molecular Biology | 2012 | 221 |
| 18 | In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus | EMBO Journal | 2013 | 220 |
| 19 | Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome | Molecular Therapy | 2016 | 204 |
| 20 | crRNA and tracrRNA guide Cas9-mediated DNA interference inStreptococcus thermophilus | RNA Biology | 2013 | 203 |
| 21 | SKEMPI 2.0: an updated benchmark of changes in protein–protein binding energy, kinetics and thermodynamics upon mutation | Bioinformatics | 2019 | 191 |
| 22 | Validation of the Crystallography Open Database using the Crystallographic Information Framework | Journal of Applied Crystallography | 2021 | 179 |
| 23 | Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements | Genome Biology | 2015 | 177 |
| 24 | PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage | Nucleic Acids Research | 2020 | 175 |
| 25 | Epigenetic dysregulation of enhancers in neurons is associated with Alzheimer’s disease pathology and cognitive symptoms | Nature Communications | 2019 | 160 |
| 26 | Doubling of the known set of RNA viruses by metagenomic analysis of an aquatic virome | Nature Microbiology | 2020 | 156 |
| 27 | Aerobic vaginitis: no longer a stranger | Research in Microbiology | 2017 | 155 |
| 28 | Signatures of plasticity, metastasis, and immunosuppression in an atlas of human small cell lung cancer | Cancer Cell | 2021 | 155 |
| 29 | VoroMQA: Assessment of protein structure quality using interatomic contact areas | Proteins: Structure, Function and Bioinformatics | 2017 | 149 |
| 30 | Directional R-Loop Formation by the CRISPR-Cas Surveillance Complex Cascade Provides Efficient Off-Target Site Rejection | Cell Reports | 2015 | 148 |
| 31 | Type III CRISPR-Cas Immunity: Major Differences Brushed Aside | Trends in Microbiology | 2017 | 133 |
| 32 | Multiple origins of prokaryotic and eukaryotic single-stranded DNA viruses from bacterial and archaeal plasmids | Nature Communications | 2019 | 127 |
| 33 | Using SMILES strings for the description of chemical connectivity in the Crystallography Open Database | Journal of Cheminformatics | 2018 | 126 |
| 34 | Mycobacterium tuberculosis Pyrazinamide Resistance Determinants: a Multicenter Study | MBio | 2014 | 125 |
| 35 | Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease | Nature | 2021 | 125 |
| 36 | CAD‐score: A new contact area difference‐based function for evaluation of protein structural models | Proteins: Structure, Function and Bioinformatics | 2013 | 123 |
| 37 | Molecular diversification of regulatory T cells in nonlymphoid tissues | Science Immunology | 2018 | 123 |
| 38 | Cressdnaviricota : a Virus Phylum Unifying Seven Families of Rep-Encoding Viruses with Single-Stranded, Circular DNA Genomes | Journal of Virology | 2020 | 118 |
| 39 | A catalogue of biochemically diverse CRISPR-Cas9 orthologs | Nature Communications | 2020 | 116 |
| 40 | Prevalence and distribution of Gardnerella vaginalis subgroups in women with and without bacterial vaginosis | BMC Infectious Diseases | 2017 | 112 |
| 41 | An Adversarial DNA N6-Methyladenine-Sensor Network Preserves Polycomb Silencing | Molecular Cell | 2019 | 109 |
| 42 | BREX system ofEscherichia colidistinguishes self from non-self by methylation of a specific DNA site | Nucleic Acids Research | 2019 | 105 |
| 43 | Blind prediction of homo‐ and hetero‐protein complexes: The CASP13‐CAPRI experiment | Proteins: Structure, Function and Bioinformatics | 2019 | 99 |
| 44 | KRAS, NRAS and BRAF mutations in colorectal cancer and melanoma | Medical Oncology | 2017 | 94 |
| 45 | Molecular mechanisms of CRISPR-mediated microbial immunity | Cellular and Molecular Life Sciences | 2014 | 93 |
| 46 | Soluble Aβ oligomers and protofibrils induce NLRP3 inflammasome activation in microglia | Journal of Neurochemistry | 2020 | 91 |
| 47 | Programmable sequence-specific click-labeling of RNA using archaeal box C/D RNP methyltransferases | Nucleic Acids Research | 2012 | 90 |
| 48 | The logic of DNA replication in double-stranded DNA viruses: insights from global analysis of viral genomes | Nucleic Acids Research | 2016 | 80 |
| 49 | Estimation of model accuracy in CASP13 | Proteins: Structure, Function and Bioinformatics | 2019 | 78 |
| 50 | CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement | Scientific Reports | 2019 | 77 |
