| # | Title | Journal | Year | Citations |
|---|
| 1 | Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method | Methods | 2001 | 145,087 |
| 2 | TrackMate: An open and extensible platform for single-particle tracking | Methods | 2017 | 2,546 |
| 3 | Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures | Methods | 2003 | 1,715 |
| 4 | Normalization of cDNA microarray data | Methods | 2003 | 1,617 |
| 5 | The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification | Methods | 2001 | 1,550 |
| 6 | Genome-wide RNAi screening in Caenorhabditis elegans | Methods | 2003 | 1,244 |
| 7 | Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals | Methods | 2007 | 1,195 |
| 8 | MEGAHIT v1.0: A fast and scalable metagenome assembler driven by advanced methodologies and community practices | Methods | 2016 | 1,174 |
| 9 | An Overview of Real-Time Quantitative PCR: Applications to Quantify Cytokine Gene Expression | Methods | 2001 | 1,150 |
| 10 | Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR) | Methods | 2010 | 1,016 |
| 11 | Analysis of gene function in somatic mammalian cells using small interfering RNAs | Methods | 2002 | 977 |
| 12 | Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes | Methods | 2012 | 943 |
| 13 | Ions from the Hofmeister series and osmolytes: effects on proteins in solution and in the crystallization process | Methods | 2004 | 920 |
| 14 | The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress | Methods | 2005 | 848 |
| 15 | Adipose-derived stem cells: Isolation, expansion and differentiation☆ | Methods | 2008 | 847 |
| 16 | Techniques to study amyloid fibril formation in vitro | Methods | 2004 | 807 |
| 17 | Hi–C: A comprehensive technique to capture the conformation of genomes | Methods | 2012 | 792 |
| 18 | The Phenix software for automated determination of macromolecular structures | Methods | 2011 | 764 |
| 19 | Inducible Gene Targeting in Mice Using the Cre/loxSystem | Methods | 1998 | 641 |
| 20 | Signal Processing in Magnetoencephalography | Methods | 2001 | 620 |
| 21 | Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis | Methods | 2014 | 599 |
| 22 | A practical approach to RT-qPCR—Publishing data that conform to the MIQE guidelines | Methods | 2010 | 591 |
| 23 | Everything you wanted to know about Markov State Models but were afraid to ask | Methods | 2010 | 591 |
| 24 | How to do successful gene expression analysis using real-time PCR | Methods | 2010 | 582 |
| 25 | Overview of manual and automated DNA sequencing by the dideoxy chain termination method | Methods | 1991 | 565 |
| 26 | Assessing sample and miRNA profile quality in serum and plasma or other biofluids | Methods | 2013 | 562 |
| 27 | Analysis of Chromatin Structure byin VivoFormaldehyde Cross-Linking | Methods | 1997 | 556 |
| 28 | Apoptosis and necrosis: Detection, discrimination and phagocytosis | Methods | 2008 | 546 |
| 29 | Sphingolipidomics: High-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry | Methods | 2005 | 542 |
| 30 | In Vivo Cross-Linking and Immunoprecipitation for Studying Dynamic Protein:DNA Associations in a Chromatin Environment | Methods | 1999 | 530 |
| 31 | Immunogenicity of engineered antibodies | Methods | 2005 | 519 |
| 32 | The absolute quantification strategy: a general procedure for the quantification of proteins and post-translational modifications | Methods | 2005 | 518 |
| 33 | Production and purification of serotype 1, 2, and 5 recombinant adeno-associated viral vectors | Methods | 2002 | 514 |
| 34 | Single-Molecule Fluorescence Resonance Energy Transfer | Methods | 2001 | 513 |
| 35 | Real-time PCR quantification of precursor and mature microRNA | Methods | 2008 | 512 |
| 36 | CLIP: A method for identifying protein–RNA interaction sites in living cells | Methods | 2005 | 509 |
| 37 | Pathogen recognition and development of particulate vaccines: Does size matter? | Methods | 2006 | 509 |
| 38 | Molecular fingerprint similarity search in virtual screening | Methods | 2015 | 506 |
| 39 | Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions | Methods | 2013 | 501 |
| 40 | Sources and measurement of ultraviolet radiation | Methods | 2002 | 487 |
| 41 | DISEASES: Text mining and data integration of disease–gene associations | Methods | 2015 | 486 |
| 42 | Virus-like particles: Passport to immune recognition | Methods | 2006 | 475 |
| 43 | Advanced transfection with Lipofectamine 2000 reagent: primary neurons, siRNA, and high-throughput applications | Methods | 2004 | 472 |
| 44 | Methods of tissue decellularization used for preparation of biologic scaffolds and in vivo relevance | Methods | 2015 | 472 |
| 45 | Simultaneous quantitative analysis of bioactive sphingolipids by high-performance liquid chromatography-tandem mass spectrometry | Methods | 2006 | 471 |
| 46 | Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay | Methods | 2008 | 466 |
| 47 | Methods of isolating extracellular vesicles impact down-stream analyses of their cargoes | Methods | 2015 | 465 |
| 48 | Circular dichroism of quadruplex DNAs: Applications to structure, cation effects and ligand binding | Methods | 2007 | 463 |
| 49 | Imaging Protein-Protein Interactions Using Fluorescence Resonance Energy Transfer Microscopy | Methods | 2001 | 460 |
| 50 | Chemical calcium indicators | Methods | 2008 | 460 |
