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Top Articles

#TitleJournalYearCitations
1Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polycrylamide to nitrocelluloseJournal of Proteomics19842,497
2Fluorescence probes used for detection of reactive oxygen speciesJournal of Proteomics20051,505
3Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCRJournal of Proteomics20001,054
4Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applicationsJournal of Proteomics1982930
5Tolerance of loop-mediated isothermal amplification to a culture medium and biological substancesJournal of Proteomics2007564
6RF cloning: A restriction-free method for inserting target genes into plasmidsJournal of Proteomics2006527
7Real-time turbidimetry of LAMP reaction for quantifying template DNAJournal of Proteomics2004517
8Perspectives of immobilized-metal affinity chromatographyJournal of Proteomics2001487
9In the absence of catalytic metals ascorbate does not autoxidize at pH 7: ascorbate as a test for catalytic metalsJournal of Proteomics1988463
10Sensitive assay of hydroxyl free radical formation utilizing high pressure liquid chromatography with electrochemical detection of phenol and salicylate hydroxylation productsJournal of Proteomics1984387
112-NBDG as a fluorescent indicator for direct glucose uptake measurementJournal of Proteomics2005373
12The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteinsJournal of Proteomics2001363
13Matrix solid phase dispersion (MSPD)Journal of Proteomics2007346
14Application of molecularly imprinted polymers to solid-phase extraction of analytes from real samplesJournal of Proteomics2007335
15Twenty-five years of immobilized metal ion affinity chromatography: past, present and futureJournal of Proteomics2001315
16A simple modification of Blum's silver stain method allows for 30 minite detection of proteins in polyacrylamide gelsJournal of Proteomics1994309
17Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganateJournal of Proteomics1985306
18Dye-ligand affinity systemsJournal of Proteomics2001285
19Oligodeoxynucleotide stability in subcellular extracts and culture mediaJournal of Proteomics1986282
20Stiffness of normal and pathological erythrocytes studied by means of atomic force microscopyJournal of Proteomics2006257
21Fluorometric method for the enzymatic determination of cholesterolJournal of Proteomics1999230
22Correction of timing errors in photomultiplier tubes used in phase-modulation fluorometryJournal of Proteomics1981226
23A non-radioactive automated method for DNA sequence determinationJournal of Proteomics1986224
24Peptide separation by Hydrophilic-Interaction Chromatography: a reviewJournal of Proteomics2004224
25Liquid-phase micro-extraction techniques in pesticide residue analysisJournal of Proteomics2007223
26Inorganic phosphate assay with malachite green: An improvement and evaluationJournal of Proteomics1982221
27Methodologies for the application of monobromobimane to the simultaneous analysis of soluble and protein thiol components of biological systemsJournal of Proteomics1986216
28Affinity membrane chromatography for the analysis and purification of proteinsJournal of Proteomics2001215
29Purification of antibodies by affinity chromatographyJournal of Proteomics2002211
30Oligodeoxynucleoside phosphorothioate stability in subcellular extracts, culture media, sera and cerebrospinal fluidJournal of Proteomics1990208
31Current trends in solid-phase-based extraction techniques for the determination of pesticides in food and environmentJournal of Proteomics2007201
32Optimisation and validation of an angiotensin-converting enzyme inhibition assay for the screening of bioactive peptidesJournal of Proteomics2002198
33Deconvolutions based on singular value decomposition and the pseudoinverse: a guide for beginnersJournal of Proteomics1994193
34Fractionation of cells and subcellular particles with PercollJournal of Proteomics2000182
35Gel gradient electrophoresis, isoelectric focusing and two-dimensional techniques in horizontal, ultrathin polycrylamide layersJournal of Proteomics1980181
36Test and calibration processes for microcalorimeters, with special reference to heat conduction instruments used with aqueous systemsJournal of Proteomics1991180
37The importance of stereoselective determination of drugs in the clinical laboratoryJournal of Proteomics2002179
38Activity of Porphyridium sp. polysaccharide against herpes simplex viruses in vitro and in vivoJournal of Proteomics2002173
39New technology for the detection of pHJournal of Proteomics2005172
40On the limiting pore size of hydrophilic gels for electrophoresis and isoelectric focussingJournal of Proteomics1981162
41A non-organic and non-enzymatic extraction method gives higher yields of genomic DNA from whole-blood samples than do nine other methods testedJournal of Proteomics1992162
42Use of Bombyx mori silk fibroin as a substratum for cultivation of animal cellsJournal of Proteomics1998158
43Antimicrobial properties of berberines alkaloids in Coptis chinensis Franch by microcalorimetryJournal of Proteomics2008157
44Manipulation and trapping of sub-micron bioparticles using dielectrophoresisJournal of Proteomics1997156
45Yet another improved silver staining method for the detection of proteins in polyacrylamide gelsJournal of Proteomics1984154
46Determination of reducing sugars in the nanomole range with tetrazolium blueJournal of Proteomics1985154
47New developments in affinity chromatography with potential application in the production of biopharmaceuticalsJournal of Proteomics2001152
48Size-exclusion chromatography—a review of calibration methodologiesJournal of Proteomics2004149
49Ionization constants of weak acids and bases in organic solventsJournal of Proteomics1999147
50Determination of surfactant critical micelle concentration by a novel fluorescence depolarization techniqueJournal of Proteomics1996146