| # | Title | Journal | Year | Citations |
|---|
| 1 | Advances in microbial amylases | Biotechnology and Applied Biochemistry | 2000 | 793 |
| 2 | The realm of microbial lipases in biotechnology | Biotechnology and Applied Biochemistry | 1999 | 381 |
| 3 | beta-1,4-D-xylan-degrading enzyme systems: biochemistry, molecular biology and applications | Biotechnology and Applied Biochemistry | 1993 | 294 |
| 4 | Computer‐aided tissue engineering: overview, scope and challenges | Biotechnology and Applied Biochemistry | 2004 | 288 |
| 5 | Colorimetric protein assay techniques | Biotechnology and Applied Biochemistry | 1999 | 288 |
| 6 | Elicitation of plants and microbial cell systems | Biotechnology and Applied Biochemistry | 2003 | 250 |
| 7 | Freeze-drying of proteins: some emerging concerns | Biotechnology and Applied Biochemistry | 2004 | 217 |
| 8 | Silk‐based materials for biomedical applications | Biotechnology and Applied Biochemistry | 2010 | 210 |
| 9 | Progress in biopharmaceutical development | Biotechnology and Applied Biochemistry | 2018 | 207 |
| 10 | Computer‐aided tissue engineering: application to biomimetic modelling and design of tissue scaffolds | Biotechnology and Applied Biochemistry | 2004 | 206 |
| 11 | Glycosylation of Pichia pastoris-derived proteins | Biotechnology and Applied Biochemistry | 1999 | 206 |
| 12 | Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2 | Biotechnology and Applied Biochemistry | 2002 | 203 |
| 13 | Development of transgenic Artemisia annua (Chinese wormwood) plants with an enhanced content of artemisinin, an effective anti‐malarial drug, by hairpin‐RNA‐mediated gene silencing | Biotechnology and Applied Biochemistry | 2009 | 193 |
| 14 | Biomaterials and scaffold design: key to tissue-engineering cartilage | Biotechnology and Applied Biochemistry | 2007 | 186 |
| 15 | A novel biomaterial: bacterial cellulose and its new era applications | Biotechnology and Applied Biochemistry | 2014 | 172 |
| 16 | Violacein: properties and biological activities | Biotechnology and Applied Biochemistry | 2007 | 169 |
| 17 | Effects of methyl jasmonate and salicylic acid on tanshinone production and biosynthetic gene expression in transgenic Salvia miltiorrhiza hairy roots | Biotechnology and Applied Biochemistry | 2015 | 161 |
| 18 | Purification and characterization of biosurfactants from Nocardia sp. L-417 | Biotechnology and Applied Biochemistry | 2000 | 151 |
| 19 | Biosynthesis and recovery of selenium nanoparticles and the effects on matrix metalloproteinase‐2 expression | Biotechnology and Applied Biochemistry | 2010 | 151 |
| 20 | Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants | Biotechnology and Applied Biochemistry | 2003 | 150 |
| 21 | Optimization of fermentation conditions for the production of bacterial cellulose by a newly isolated Acetobacter sp.A9 in shaking cultures | Biotechnology and Applied Biochemistry | 2001 | 147 |
| 22 | Lipase assays for conventional and molecular screening: an overview | Biotechnology and Applied Biochemistry | 2003 | 146 |
| 23 | The requirement of lipids for the formation of immunostimulating complexes (iscoms) | Biotechnology and Applied Biochemistry | 1988 | 146 |
| 24 | Plant‐made pharmaceuticals: Leading products and production platforms | Biotechnology and Applied Biochemistry | 2011 | 143 |
| 25 | Stem-cell plasticity and therapy for injuries of the peripheral nervous system | Biotechnology and Applied Biochemistry | 2004 | 142 |
| 26 | Recombinant production of antimicrobial peptides in heterologous microbial systems | Biotechnology and Applied Biochemistry | 2007 | 142 |
| 27 | Immobilization of bone morphogenetic protein-2 on a nanofibrous chitosan membrane for enhanced guided bone regeneration | Biotechnology and Applied Biochemistry | 2006 | 141 |
| 28 | Caspase‐3: Structure, function, and biotechnological aspects | Biotechnology and Applied Biochemistry | 2022 | 134 |
| 29 | Design and production of recombinant subunit vaccines | Biotechnology and Applied Biochemistry | 2000 | 131 |
| 30 | The interplay of ROS and the PI3K/Akt pathway in autophagy regulation | Biotechnology and Applied Biochemistry | 2022 | 130 |
| 31 | Tissue‐engineered skin substitutes: from in vitro constructs to in vivo applications | Biotechnology and Applied Biochemistry | 2004 | 128 |
| 32 | Modified alginate matrices for the immobilization of bioactive agents | Biotechnology and Applied Biochemistry | 2004 | 122 |
| 33 | A review on the important aspects of lipase immobilization on nanomaterials | Biotechnology and Applied Biochemistry | 2017 | 120 |
| 34 | Immobilized lipase-catalysed production of alkyl esters of restaurant grease as biodiesel | Biotechnology and Applied Biochemistry | 2002 | 117 |
| 35 | Evaluation of an ion-exchange membrane for the purification of plasmid DNA | Biotechnology and Applied Biochemistry | 2003 | 115 |
| 36 | Carrier proteins for fusion expression of antimicrobial peptides in Escherichia coli | Biotechnology and Applied Biochemistry | 2009 | 115 |
| 37 | Engineering mammalian cells in bioprocessing – current achievements and future perspectives | Biotechnology and Applied Biochemistry | 2010 | 115 |
| 38 | Bacillus minimum genome factory: effective utilization of microbial genome information | Biotechnology and Applied Biochemistry | 2007 | 114 |
| 39 | Properties and antityrosinase activity of sericin from various extraction methods | Biotechnology and Applied Biochemistry | 2010 | 113 |
| 40 | Evaluation of a new reagent for covalent attachment of polyethylene glycol to proteins.“ | Biotechnology and Applied Biochemistry | 1992 | 109 |
| 41 | The requirement of lipids for the formation of immunostimulating complexes (iscoms) | Biotechnology and Applied Biochemistry | 1988 | 108 |
| 42 | Green synthesis of gold nanoparticles by the marine microalga Tetraselmis suecica | Biotechnology and Applied Biochemistry | 2010 | 106 |
| 43 | Tissue engineering of blood vessels: characterization of smooth-muscle cells for culturing on collagen-and-elastin-based scaffolds | Biotechnology and Applied Biochemistry | 2004 | 105 |
| 44 | Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli | Biotechnology and Applied Biochemistry | 2002 | 103 |
| 45 | Phytoremediation of 2,4-dichlorophenol by Brassica napus hairy root cultures | Biotechnology and Applied Biochemistry | 2003 | 103 |
| 46 | An Aspergillus chitosanase with potential for large-scale preparation of chitosan oligosaccharides | Biotechnology and Applied Biochemistry | 2000 | 100 |
| 47 | Human adult craniofacial muscle-derived cells: neural-cell adhesion-molecule (NCAM; CD56)-expressing cells appear to contain multipotential stem cells | Biotechnology and Applied Biochemistry | 2004 | 100 |
| 48 | Biosensors: Classifications, medical applications, and future prospective | Biotechnology and Applied Biochemistry | 2018 | 99 |
| 49 | Taxol production by Pestalotiopsis terminaliae, an endophytic fungus of Terminalia arjuna (arjun tree) | Biotechnology and Applied Biochemistry | 2009 | 98 |
| 50 | Towards molecular farming in the future: transient protein expression in plants | Biotechnology and Applied Biochemistry | 1999 | 98 |
