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Nature Protocols
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Top Articles
Nature Protocols
18.8
(top 1%)
Impact Factor
20.1
(top 1%)
extended IF
289
(top 1%)
H-Index
5.3K
authors
3.2K
papers
454.2K
citations
9.3K
citing journals
331.9K
citing authors
Most Cited Articles of Nature Protocols
Title
Year
Citations
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
2009
24.4K
Analyzing real-time PCR data by the comparative C(T) method
2008
15.9K
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
2012
8.3K
Genome engineering using the CRISPR-Cas9 system
2013
6.2K
The Phyre2 web portal for protein modeling, prediction and analysis
2015
5.6K
Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm
2009
5.1K
De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis
2013
4.6K
I-TASSER: a unified platform for automated protein structure and function prediction
2010
4.4K
Protein structure prediction on the Web: a case study using the Phyre server
2009
3.6K
In-gel digestion for mass spectrometric characterization of proteins and proteomes
2006
3.4K
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis
2007
2.9K
Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances
2008
2.9K
In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro
2007
2.9K
Locating proteins in the cell using TargetP, SignalP and related tools
2007
2.6K
Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips
2007
2.5K
Morris water maze: procedures for assessing spatial and related forms of learning and memory
2006
2.5K
Clonogenic assay of cells in vitro
2006
2.5K
Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown
2016
2.3K
Using circular dichroism spectra to estimate protein secondary structure
2006
2.2K
Sulforhodamine B colorimetric assay for cytotoxicity screening
2006
2.2K
Full-length RNA-seq from single cells using Smart-seq2
2014
2K
Tricine-SDS-PAGE
2006
1.8K
Integration of biological networks and gene expression data using Cytoscape
2007
1.8K
Materials fabrication from Bombyx mori silk fibroin
2011
1.8K
High-resolution in situ hybridization to whole-mount zebrafish embryos
2008
1.7K
previous
2009
2010
2011
How are inpact factors calculated?
The impact factor (IF) is calculated by counting citations from peer-reviewed journals only.
extended IF
also counts citations from books and conference papers. However, no patent, abstract, working papers, online documents, etc., are covered.
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