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Top Articles

#TitleJournalYearCitations
1Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiaeYeast19984,800
2Designer deletion strains derived fromSaccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applicationsYeast19983,028
3New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiaeYeast19942,547
4Heterologous modules for efficient and versatile PCR-based gene targeting inSchizosaccharomyces pombeYeast19982,105
5Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedureYeast19951,939
6A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettesYeast20041,837
7Three new dominant drug resistance cassettes for gene disruption inSaccharomyces cerevisiaeYeast19991,673
8Yeast/E. coli shuttle vectors with multiple unique restriction sitesYeast19861,471
9Effect of benzoic acid on metabolic fluxes in yeasts: A continuous-culture study on the regulation of respiration and alcoholic fermentationYeast19921,291
10Construction of a set of convenientsaccharomyces cerevisiae strains that are isogenic to S288CYeast19951,162
11Heterologous protein production using thePichia pastoris expression systemYeast20051,054
12Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemakingYeast20001,051
13Foreign gene expression in yeast: a reviewYeast1992948
14Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routinesYeast1999946
15Rapid and reliable protein extraction from yeastYeast2000850
16PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiaeYeast1996763
17Pyruvate Metabolism inSaccharomyces cerevisiaeYeast1996650
18Optimized cassettes for fluorescent protein tagging inSaccharomyces cerevisiaeYeast2004639
19Cell wall construction inSaccharomyces cerevisiaeYeast2006617
20HeterologousHIS3 Marker and GFP Reporter Modules for PCR-Targeting inSaccharomyces cerevisiaeYeast1997584
21A family of low and high copy replicative, integrative and single-strandedS. cerevisiae/E. coli shuttle vectorsYeast1991570
22Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiaeYeast1998564
23Review: Cell wall assembly in yeastYeast1994529
24A rapid method for localized mutagenesis of yeast genesYeast1992481
25Basic methods for fission yeastYeast2006457
26Sequences important for gene expression in filamentous fungiYeast1986425
27Applications of high efficiency lithium acetate transformation of intact yeast cells using single‐stranded nucleic acids as carrierYeast1991424
28A suite of Gateway®cloning vectors for high-throughput genetic analysis inSaccharomyces cerevisiaeYeast2007419
29YeastSaccharomyces cerevisiae selectable markers in pUC18 polylinkersYeast1990418
30PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiaeYeast1996401
31An efficient transformation procedure enabling long-term storage of competent cells of various yeast generaYeast1991391
32The YDp plasmids: A uniform set of vectors bearing versatile gene disruption cassettes forSaccharomyces cerevisiaeYeast1991372
33Global metabolite analysis of yeast: evaluation of sample preparation methodsYeast2005365
34Mitochondria-targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis inSaccharomyces cerevisiaeYeast2000358
35Signal transduction in yeastYeast1994353
36Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeastYeast1996344
37An expanded tool kit for the auxin‐inducible degron system in budding yeastYeast2013342
38Isolation and biochemical characterization of organelles from the yeast,Saccharomyces cerevisiaeYeast1995340
39Assessment of prediction accuracy of protein function from protein-protein interaction dataYeast2001337
40Proliferation of microbodies inSaccharomyces cerevisiaeYeast1987323
41Genome-wide monitoring of wine yeast gene expression during alcoholic fermentationYeast2003316
42Genetic map ofSaccharomyces cerevisiae, edition 10Yeast1989312
43A new approach for isolating cell wall mutants inSaccharomyces cerevisiae by screening for hypersensitivity to calcofluor whiteYeast1994311
44Plasmids with the Cre-recombinase and the dominantnat marker, suitable for use in prototrophic strains ofSaccharomyces cerevisiae andKluyveromyces lactisYeast2001311
45Use of polymerase chain reaction epitope tagging for protein tagging inSaccharomyces cerevisiaeYeast1995307
46CIp10, an efficient and convenient integrating vector for Candida albicansYeast2000294
47Vector systems for the expression, analysis and cloning of DNA sequence inS. cerevisiaeYeast1985293
48Overproduction of glycolytic enzymes in yeastYeast1989291
49Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeastYeast1993289
50Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiaeYeast2010281