| # | Title | Journal | Year | Citations |
|---|
| 1 | Improving Bioscience Research Reporting: The ARRIVE Guidelines for Reporting Animal Research | PLoS Biology | 2010 | 5,621 |
| 2 | Relaxed Phylogenetics and Dating with Confidence | PLoS Biology | 2006 | 5,566 |
| 3 | Mapping the Structural Core of Human Cerebral Cortex | PLoS Biology | 2008 | 3,556 |
| 4 | Revised Estimates for the Number of Human and Bacteria Cells in the Body | PLoS Biology | 2016 | 3,388 |
| 5 | Human MicroRNA Targets | PLoS Biology | 2004 | 3,253 |
| 6 | Senescence-Associated Secretory Phenotypes Reveal Cell-Nonautonomous Functions of Oncogenic RAS and the p53 Tumor Suppressor | PLoS Biology | 2008 | 3,067 |
| 7 | Development of the Human Infant Intestinal Microbiota | PLoS Biology | 2007 | 2,390 |
| 8 | A Map of Recent Positive Selection in the Human Genome | PLoS Biology | 2006 | 2,329 |
| 9 | PINK1 Is Selectively Stabilized on Impaired Mitochondria to Activate Parkin | PLoS Biology | 2010 | 2,299 |
| 10 | The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research | PLoS Biology | 2020 | 2,209 |
| 11 | Principles of MicroRNA–Target Recognition | PLoS Biology | 2005 | 2,019 |
| 12 | The Pervasive Effects of an Antibiotic on the Human Gut Microbiota, as Revealed by Deep 16S rRNA Sequencing | PLoS Biology | 2008 | 2,013 |
| 13 | How Many Species Are There on Earth and in the Ocean? | PLoS Biology | 2011 | 1,970 |
| 14 | Identification of Birds through DNA Barcodes | PLoS Biology | 2004 | 1,920 |
| 15 | The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific | PLoS Biology | 2007 | 1,757 |
| 16 | DNA Detection Using Recombination Proteins | PLoS Biology | 2006 | 1,652 |
| 17 | CellProfiler 3.0: Next-generation image processing for biology | PLoS Biology | 2018 | 1,547 |
| 18 | Stochastic mRNA Synthesis in Mammalian Cells | PLoS Biology | 2006 | 1,528 |
| 19 | The Diploid Genome Sequence of an Individual Human | PLoS Biology | 2007 | 1,491 |
| 20 | Adaptation, Plasticity, and Extinction in a Changing Environment: Towards a Predictive Theory | PLoS Biology | 2010 | 1,476 |
| 21 | Grasping the Intentions of Others with One's Own Mirror Neuron System | PLoS Biology | 2005 | 1,452 |
| 22 | The Transcriptome of the Intraerythrocytic Developmental Cycle of Plasmodium falciparum | PLoS Biology | 2003 | 1,433 |
| 23 | Learning to Control a Brain–Machine Interface for Reaching and Grasping by Primates | PLoS Biology | 2003 | 1,427 |
| 24 | DNA Barcoding: Error Rates Based on Comprehensive Sampling | PLoS Biology | 2005 | 1,398 |
| 25 | Serial Block-Face Scanning Electron Microscopy to Reconstruct Three-Dimensional Tissue Nanostructure | PLoS Biology | 2004 | 1,381 |
| 26 | Genetic and Functional Diversification of Small RNA Pathways in Plants | PLoS Biology | 2004 | 1,347 |
| 27 | Large-Scale Mapping and Validation of Escherichia coli Transcriptional Regulation from a Compendium of Expression Profiles | PLoS Biology | 2007 | 1,308 |
| 28 | Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate | PLoS Biology | 2005 | 1,280 |
| 29 | A User's Guide to the Encyclopedia of DNA Elements (ENCODE) | PLoS Biology | 2011 | 1,257 |
| 30 | Highly Nonrandom Features of Synaptic Connectivity in Local Cortical Circuits | PLoS Biology | 2005 | 1,222 |
| 31 | Microglial Interactions with Synapses Are Modulated by Visual Experience | PLoS Biology | 2010 | 1,217 |
| 32 | Purified Wnt5a Protein Activates or Inhibits β-Catenin–TCF Signaling Depending on Receptor Context | PLoS Biology | 2006 | 1,101 |
| 33 | Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0 | PLoS Biology | 2020 | 1,069 |
| 34 | Epigenetic Control of the foxp3 Locus in Regulatory T Cells | PLoS Biology | 2007 | 1,068 |
| 35 | Microbial Hub Taxa Link Host and Abiotic Factors to Plant Microbiome Variation | PLoS Biology | 2016 | 1,065 |
| 36 | Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation | PLoS Biology | 2012 | 1,064 |
| 37 | The Bacterial Symbiont Wolbachia Induces Resistance to RNA Viral Infections in Drosophila melanogaster | PLoS Biology | 2008 | 999 |
| 38 | Big Data: Astronomical or Genomical? | PLoS Biology | 2015 | 995 |
| 39 | Biodiversity Loss Threatens Human Well-Being | PLoS Biology | 2006 | 984 |
| 40 | Active-Site Inhibitors of mTOR Target Rapamycin-Resistant Outputs of mTORC1 and mTORC2 | PLoS Biology | 2009 | 973 |
| 41 | Interacting Adaptive Processes with Different Timescales Underlie Short-Term Motor Learning | PLoS Biology | 2006 | 953 |
| 42 | A Developmental Switch in the Response of DRG Neurons to ETS Transcription Factor Signaling | PLoS Biology | 2005 | 951 |
| 43 | Regulation of Muscle Fiber Type and Running Endurance by PPARδ | PLoS Biology | 2004 | 932 |
| 44 | Resolving Difficult Phylogenetic Questions: Why More Sequences Are Not Enough | PLoS Biology | 2011 | 932 |
| 45 | Sex Determination: Why So Many Ways of Doing It? | PLoS Biology | 2014 | 916 |
| 46 | Genome Sequence of the Pea Aphid Acyrthosiphon pisum | PLoS Biology | 2010 | 913 |
| 47 | Salmonella enterica Serovar Typhimurium Exploits Inflammation to Compete with the Intestinal Microbiota | PLoS Biology | 2007 | 905 |
| 48 | The Pattern of Polymorphism in Arabidopsis thaliana | PLoS Biology | 2005 | 895 |
| 49 | Money for Nothing? A Call for Empirical Evaluation of Biodiversity Conservation Investments | PLoS Biology | 2006 | 891 |
| 50 | The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing | PLoS Biology | 2014 | 885 |
