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Top Articles

#TitleJournalYearCitations
1Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research199765,304
2CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research199459,778
3MUSCLE: multiple sequence alignment with high accuracy and high throughputNucleic Acids Research200438,269
4The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis toolsNucleic Acids Research199735,199
5The Protein Data BankNucleic Acids Research200032,233
6A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research200129,318
7limma powers differential expression analyses for RNA-sequencing and microarray studiesNucleic Acids Research201528,276
8KEGG: Kyoto Encyclopedia of Genes and GenomesNucleic Acids Research200027,083
9The SILVA ribosomal RNA gene database project: improved data processing and web-based toolsNucleic Acids Research201223,218
10A simple salting out procedure for extracting DNA from human nucleated cellsNucleic Acids Research198817,472
11A comprehensive set of sequence analysis programs for the VAXNucleic Acids Research198415,681
12A rapid alkaline extraction procedure for screening recombinant plasmid DNANucleic Acids Research197915,458
13KEGG: Kyoto Encyclopedia of Genes and GenomesNucleic Acids Research199914,416
14Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene listsNucleic Acids Research200913,565
15MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transformNucleic Acids Research200213,259
16STRING v11: protein–protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasetsNucleic Acids Research201913,235
17Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nucleiNucleic Acids Research198312,088
18Mfold web server for nucleic acid folding and hybridization predictionNucleic Acids Research200311,697
19ANNOVAR: functional annotation of genetic variants from high-throughput sequencing dataNucleic Acids Research201011,615
20Gene Expression Omnibus: NCBI gene expression and hybridization array data repositoryNucleic Acids Research200211,338
21DNA polymorphisms amplified by arbitrary primers are useful as genetic markersNucleic Acids Research199010,929
22AFLP: a new technique for DNA fingerprintingNucleic Acids Research199510,927
23Rapid isolation of high molecular weight plant DNANucleic Acids Research198010,546
24tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic SequenceNucleic Acids Research19979,657
25STRING v10: protein–protein interaction networks, integrated over the tree of lifeNucleic Acids Research20159,399
26SWISS-MODEL: homology modelling of protein structures and complexesNucleic Acids Research20189,233
27MEME SUITE: tools for motif discovery and searchingNucleic Acids Research20098,571
28Enrichr: a comprehensive gene set enrichment analysis web server 2016 updateNucleic Acids Research20167,874
29Primer3—new capabilities and interfacesNucleic Acids Research20127,850
30The Pfam protein families databaseNucleic Acids Research20077,778
31NCBI GEO: archive for functional genomics data sets—updateNucleic Acids Research20127,638
32GEPIA: a web server for cancer and normal gene expression profiling and interactive analysesNucleic Acids Research20177,580
33Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterNucleic Acids Research19847,213
34Tandem repeats finder: a program to analyze DNA sequencesNucleic Acids Research19997,171
35KEGG: new perspectives on genomes, pathways, diseases and drugsNucleic Acids Research20177,061
36Loop-mediated isothermal amplification of DNANucleic Acids Research20006,947
37Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studiesNucleic Acids Research20136,678
38The PRIDE database and related tools and resources in 2019: improving support for quantification dataNucleic Acids Research20196,663
39UniProt: a worldwide hub of protein knowledgeNucleic Acids Research20196,531
40Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCRNucleic Acids Research20026,526
41The STRING database in 2017: quality-controlled protein–protein association networks, made broadly accessibleNucleic Acids Research20176,501
42dbSNP: the NCBI database of genetic variationNucleic Acids Research20016,288
43The Genomes On Line Database (GOLD) in 2009: status of genomic and metagenomic projects and their associated metadataNucleic Acids Research20106,190
44Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotationNucleic Acids Research20216,174
45DrugBank 5.0: a major update to the DrugBank database for 2018Nucleic Acids Research20185,962
46SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARBNucleic Acids Research20075,955
47ARB: a software environment for sequence dataNucleic Acids Research20045,866
48KEGG as a reference resource for gene and protein annotationNucleic Acids Research20165,732
49deepTools2: a next generation web server for deep-sequencing data analysisNucleic Acids Research20165,724
50KEGG for linking genomes to life and the environmentNucleic Acids Research20075,634