| # | Title | Journal | Year | Citations |
|---|
| 1 | Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction | Analytical Biochemistry | 1987 | 58,438 |
| 2 | Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction | Analytical Biochemistry | 1987 | 27,111 |
| 3 | Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) | Autophagy | 2016 | 4,701 |
| 4 | Production of infectious hepatitis C virus in tissue culture from a cloned viral genome | Nature Medicine | 2005 | 2,561 |
| 5 | Lymphocyte egress from thymus and peripheral lymphoid organs is dependent on S1P receptor 1 | Nature | 2004 | 2,348 |
| 6 | Amount of Time Spent in Sedentary Behaviors in the United States, 2003-2004 | American Journal of Epidemiology | 2008 | 2,093 |
| 7 | Backbone dynamics of proteins as studied by nitrogen-15 inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease | Biochemistry | 1989 | 1,856 |
| 8 | Activating Mutations of the Stimulatory G Protein in the McCune–Albright Syndrome | New England Journal of Medicine | 1991 | 1,804 |
| 9 | The metabolic syndrome in children and adolescents ? an IDF consensus report | Pediatric Diabetes | 2007 | 1,509 |
| 10 | The long-term effect of lifestyle interventions to prevent diabetes in the China Da Qing Diabetes Prevention Study: a 20-year follow-up study | Lancet, The | 2008 | 1,308 |
| 11 | The Influence of Macromolecular Crowding and Macromolecular Confinement on Biochemical Reactions in Physiological Media | Journal of Biological Chemistry | 2001 | 1,263 |
| 12 | Genomic Instability in Mice Lacking Histone H2AX | Science | 2002 | 1,263 |
| 13 | Mechanism by Which Fatty Acids Inhibit Insulin Activation of Insulin Receptor Substrate-1 (IRS-1)-associated Phosphatidylinositol 3-Kinase Activity in Muscle | Journal of Biological Chemistry | 2002 | 1,254 |
| 14 | Prevalence of Type 1 and Type 2 Diabetes Among Children and Adolescents From 2001 to 2009 | JAMA - Journal of the American Medical Association | 2014 | 1,160 |
| 15 | Onset of NIDDM occurs at Least 4-7 yr Before Clinical Diagnosis | Diabetes Care | 1992 | 1,159 |
| 16 | Adipose-derived circulating miRNAs regulate gene expression in other tissues | Nature | 2017 | 1,107 |
| 17 | The liver as an immunological organ | Hepatology | 2006 | 1,076 |
| 18 | Effect of Weight Loss With Lifestyle Intervention on Risk of Diabetes | Diabetes Care | 2006 | 1,050 |
| 19 | Edg-1, the G protein–coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation | Journal of Clinical Investigation | 2000 | 1,045 |
| 20 | A Class of Membrane Proteins Shaping the Tubular Endoplasmic Reticulum | Cell | 2006 | 1,005 |
| 21 | Annexins: the problem of assessing the biological role for a gene family of multifunctional calcium- and phospholipid-binding proteins | BBA - Biomembranes | 1994 | 998 |
| 22 | Chemical Inhibition of the Mitochondrial Division Dynamin Reveals Its Role in Bax/Bak-Dependent Mitochondrial Outer Membrane Permeabilization | Developmental Cell | 2008 | 992 |
| 23 | Mutations in the human Ca2+-sensing receptor gene cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism | Cell | 1993 | 991 |
| 24 | Measurement ofJand Dipolar Couplings from Simplified Two-Dimensional NMR Spectra | Journal of Magnetic Resonance | 1998 | 931 |
| 25 | A novel approach for sequential assignment of proton, carbon-13, and nitrogen-15 spectra of larger proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin | Biochemistry | 1990 | 926 |
| 26 | Mice lacking Smad3 show accelerated wound healing and an impaired local inflammatory response | Nature Cell Biology | 1999 | 842 |
| 27 | Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases | Science | 1994 | 830 |
| 28 | Amphetamine-type central nervous system stimulants release norepinephrine more potently than they release dopamine and serotonin | Synapse | 2001 | 825 |
| 29 | Structure and Dynamics of Micelle-bound Human α-Synuclein | Journal of Biological Chemistry | 2005 | 803 |
| 30 | Free energy reconstruction from nonequilibrium single-molecule pulling experiments | Proceedings of the National Academy of Sciences of the United States of America | 2001 | 800 |
| 31 | Structure of an Agonist-Bound Human A 2A Adenosine Receptor | Science | 2011 | 783 |
| 32 | Activation and allosteric modulation of a muscarinic acetylcholine receptor | Nature | 2013 | 779 |
| 33 | The metabolic syndrome in children and adolescents | Lancet, The | 2007 | 776 |
| 34 | Specific and Nonhepatotoxic Degradation of Nuclear Hepatitis B Virus cccDNA | Science | 2014 | 774 |
| 35 | Immune dysregulation in human subjects with heterozygous germline mutations in CTLA4 | Science | 2014 | 745 |
| 36 | A constitutively activating mutation of the luteinizing hormone receptor in familial male precocious puberty | Nature | 1993 | 743 |
| 37 | Structure and dynamics of the M3 muscarinic acetylcholine receptor | Nature | 2012 | 714 |
| 38 | Evolution of accelerometer methods for physical activity research | British Journal of Sports Medicine | 2014 | 710 |
| 39 | SIRT3 Is a Mitochondria-Localized Tumor Suppressor Required for Maintenance of Mitochondrial Integrity and Metabolism during Stress | Cancer Cell | 2010 | 705 |
| 40 | Prevalence and ethnic differences in gallbladder disease in the United States | Gastroenterology | 1999 | 701 |
| 41 | Long-term Drug Treatment for Obesity | JAMA - Journal of the American Medical Association | 2014 | 697 |
| 42 | The Burden of Mortality Attributable to Diabetes: Realistic estimates for the year 2000 | Diabetes Care | 2005 | 693 |
| 43 | Uncoupling Protein-3 Is a Mediator of Thermogenesis Regulated by Thyroid Hormone, β3-Adrenergic Agonists, and Leptin | Journal of Biological Chemistry | 1997 | 687 |
| 44 | HRPT2, encoding parafibromin, is mutated in hyperparathyroidism–jaw tumor syndrome | Nature Genetics | 2002 | 686 |
| 45 | Essential Role for Sphingosine Kinases in Neural and Vascular Development | Molecular and Cellular Biology | 2005 | 683 |
| 46 | Liver Peroxisome Proliferator-activated Receptor γ Contributes to Hepatic Steatosis, Triglyceride Clearance, and Regulation of Body Fat Mass | Journal of Biological Chemistry | 2003 | 672 |
| 47 | Structural Basis of Toll-Like Receptor 3 Signaling with Double-Stranded RNA | Science | 2008 | 650 |
| 48 | Exosomes from human saliva as a source of microRNA biomarkers | Oral Diseases | 2010 | 650 |
| 49 | Burden of Digestive Diseases in the United States Part III: Liver, Biliary Tract, and Pancreas | Gastroenterology | 2009 | 645 |
| 50 | The Prevalence of Meeting A1C, Blood Pressure, and LDL Goals Among People With Diabetes, 1988–2010 | Diabetes Care | 2013 | 620 |
