| # | Title | Journal | Year | Citations |
|---|
| 1 | Basic local alignment search tool | Journal of Molecular Biology | 1990 | 82,180 |
| 2 | Gapped BLAST and PSI-BLAST: a new generation of protein database search programs | Nucleic Acids Research | 1997 | 64,420 |
| 3 | Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction | Analytical Biochemistry | 1987 | 58,438 |
| 4 | NIH Image to ImageJ: 25 years of image analysis | Nature Methods | 2012 | 46,756 |
| 5 | Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction | Analytical Biochemistry | 1987 | 27,111 |
| 6 | A New Equation to Estimate Glomerular Filtration Rate | Annals of Internal Medicine | 2009 | 19,025 |
| 7 | BLAST+: architecture and applications | BMC Bioinformatics | 2009 | 14,935 |
| 8 | Risks and Benefits of Estrogen Plus Progestin in Healthy Postmenopausal Women: Principal Results From the Women's Health Initiative Randomized Controlled Trial | JAMA - Journal of the American Medical Association | 2002 | 14,536 |
| 9 | NMRPipe: A multidimensional spectral processing system based on UNIX pipes | Journal of Biomolecular NMR | 1995 | 14,090 |
| 10 | Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure | Hypertension | 2003 | 11,852 |
| 11 | Gene Expression Omnibus: NCBI gene expression and hybridization array data repository | Nucleic Acids Research | 2002 | 10,953 |
| 12 | A comparative risk assessment of burden of disease and injury attributable to 67 risk factors and risk factor clusters in 21 regions, 1990–2010: a systematic analysis for the Global Burden of Disease Study 2010 | Lancet, The | 2012 | 9,397 |
| 13 | Global, regional, and national prevalence of overweight and obesity in children and adults during 1980–2013: a systematic analysis for the Global Burden of Disease Study 2013 | Lancet, The | 2014 | 9,122 |
| 14 | Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling | Nature | 2000 | 8,977 |
| 15 | LIGAND: A versatile computerized approach for characterization of ligand-binding systems | Analytical Biochemistry | 1980 | 8,732 |
| 16 | The FAIR Guiding Principles for scientific data management and stewardship | Scientific Data | 2016 | 8,670 |
| 17 | Second-generation PLINK: rising to the challenge of larger and richer datasets | GigaScience | 2015 | 8,062 |
| 18 | p53 mutations in human cancers | Science | 1991 | 7,591 |
| 19 | Mutation in the -Synuclein Gene Identified in Families with Parkinson's Disease | Science | 1997 | 7,557 |
| 20 | Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation | Science | 2020 | 7,517 |
| 21 | Finding the missing heritability of complex diseases | Nature | 2009 | 7,490 |
| 22 | Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence | Nature | 1998 | 7,357 |
| 23 | An integrated map of genetic variation from 1,092 human genomes | Nature | 2012 | 7,199 |
| 24 | NCBI GEO: archive for functional genomics data sets—update | Nucleic Acids Research | 2012 | 7,174 |
| 25 | DAVID: Database for Annotation, Visualization, and Integrated Discovery | Genome Biology | 2003 | 7,168 |
| 26 | A Short Physical Performance Battery Assessing Lower Extremity Function: Association With Self-Reported Disability and Prediction of Mortality and Nursing Home Admission | Journal of Gerontology | 1994 | 7,145 |
| 27 | The global distribution and burden of dengue | Nature | 2013 | 7,138 |
| 28 | Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose | Proceedings of the National Academy of Sciences of the United States of America | 1972 | 7,134 |
| 29 | Effects of Intensive Glucose Lowering in Type 2 Diabetes | New England Journal of Medicine | 2008 | 7,084 |
| 30 | CHARMM: The biomolecular simulation program | Journal of Computational Chemistry | 2009 | 7,077 |
| 31 | Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines | Journal of Extracellular Vesicles | 2018 | 6,961 |
| 32 | The Genotype-Tissue Expression (GTEx) project | Nature Genetics | 2013 | 6,815 |
| 33 | Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990–2010: a systematic analysis for the Global Burden of Disease Study 2010 | Lancet, The | 2012 | 6,376 |
| 34 | The Cancer Genome Atlas Pan-Cancer analysis project | Nature Genetics | 2013 | 6,265 |
| 35 | Recommended diagnostic criteria for multiple sclerosis: Guidelines from the international panel on the diagnosis of multiple sclerosis | Annals of Neurology | 2001 | 6,122 |
| 36 | Principles that Govern the Folding of Protein Chains | Science | 1973 | 6,106 |
| 37 | dbSNP: the NCBI database of genetic variation | Nucleic Acids Research | 2001 | 6,103 |
| 38 | Physical Activity in the United States Measured by Accelerometer | Medicine and Science in Sports and Exercise | 2008 | 6,026 |
| 39 | The Thiobarbituric Acid Assay of Sialic Acids | Journal of Biological Chemistry | 1959 | 5,961 |
| 40 | Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues | Analytical Biochemistry | 1969 | 5,750 |
| 41 | A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1 | Science | 1994 | 5,747 |
| 42 | Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation | Genome Research | 2017 | 5,620 |
| 43 | Effectiveness of Antipsychotic Drugs in Patients with Chronic Schizophrenia | New England Journal of Medicine | 2005 | 5,335 |
| 44 | Proposals for the Classification of the Acute Leukaemias French‐American‐British (FAB) Co‐operative Group | British Journal of Haematology | 1976 | 5,316 |
| 45 | A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures | Journal of Biological Chemistry | 1961 | 5,290 |
| 46 | MR diffusion tensor spectroscopy and imaging | Biophysical Journal | 1994 | 5,262 |
| 47 | A microRNA expression signature of human solid tumors defines cancer gene targets | Proceedings of the National Academy of Sciences of the United States of America | 2006 | 5,220 |
| 48 | Nitric Oxide and Peroxynitrite in Health and Disease | Physiological Reviews | 2007 | 5,209 |
| 49 | Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1 | Science | 2009 | 4,989 |
| 50 | Trastuzumab plus Adjuvant Chemotherapy for Operable HER2-Positive Breast Cancer | New England Journal of Medicine | 2005 | 4,956 |
