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#	Title	Journal	Year	Citations
1	Full-length transcriptome assembly from RNA-Seq data without a reference genome	Nature Biotechnology	2011	18,090
2	Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation	Nature Biotechnology	2010	14,198
3	Pseudo-second order model for sorption processes	Process Biochemistry	1999	13,746
4	MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification	Nature Biotechnology	2008	13,477
5	Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2	Nature Biotechnology	2019	12,956
6	Integrative genomics viewer	Nature Biotechnology	2011	12,409
7	Superior Thermal Conductivity of Single-Layer Graphene	Nano Letters	2008	12,066
8	Integrating single-cell transcriptomic data across different conditions, technologies, and species	Nature Biotechnology	2018	9,679
9	StringTie enables improved reconstruction of a transcriptome from RNA-seq reads	Nature Biotechnology	2015	9,345
10	Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences	Nature Biotechnology	2013	8,414
11	Emerging Photoluminescence in Monolayer MoS₂	Nano Letters	2010	8,157
12	Near-optimal probabilistic RNA-seq quantification	Nature Biotechnology	2016	7,902
13	Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype	Nature Biotechnology	2019	7,823
14	Graphene-Based Ultracapacitors	Nano Letters	2008	7,714
15	Nanocrystals of Cesium Lead Halide Perovskites (CsPbX₃, X = Cl, Br, and I): Novel Optoelectronic Materials Showing Bright Emission with Wide Color Gamut	Nano Letters	2015	7,300
16	3D bioprinting of tissues and organs	Nature Biotechnology	2014	5,401
17	Large Area, Few-Layer Graphene Films on Arbitrary Substrates by Chemical Vapor Deposition	Nano Letters	2009	5,288
18	Principles of nanoparticle design for overcoming biological barriers to drug delivery	Nature Biotechnology	2015	5,199
19	The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells	Nature Biotechnology	2014	5,076
20	Spatial reconstruction of single-cell gene expression data	Nature Biotechnology	2015	4,733
21	Quantitative analysis of complex protein mixtures using isotope-coded affinity tags	Nature Biotechnology	1999	4,687
22	Large-scale analysis of the yeast proteome by multidimensional protein identification technology	Nature Biotechnology	2001	4,566
23	In vivo cancer targeting and imaging with semiconductor quantum dots	Nature Biotechnology	2004	4,504
24	The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution	Journal of Plant Physiology	1994	4,332
25	Determining the Size and Shape Dependence of Gold Nanoparticle Uptake into Mammalian Cells	Nano Letters	2006	4,324
26	Chemical Management for Colorful, Efficient, and Stable Inorganic–Organic Hybrid Nanostructured Solar Cells	Nano Letters	2013	4,264
27	Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein	Nature Biotechnology	2004	4,214
28	Transparent, Conductive Graphene Electrodes for Dye-Sensitized Solar Cells	Nano Letters	2008	4,206
29	Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering	Nature Biotechnology	2005	4,130
30	Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples	Nature Biotechnology	2013	4,096
31	DNA targeting specificity of RNA-guided Cas9 nucleases	Nature Biotechnology	2013	4,085
32	Raman Spectra of Graphite Oxide and Functionalized Graphene Sheets	Nano Letters	2008	4,078
33	Molecular Beacons: Probes that Fluoresce upon Hybridization	Nature Biotechnology	1996	3,987
34	GREAT improves functional interpretation of cis-regulatory regions	Nature Biotechnology	2010	3,977
35	Renal clearance of quantum dots	Nature Biotechnology	2007	3,893
36	Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes	Nature Biotechnology	2011	3,784
37	Next-generation DNA sequencing	Nature Biotechnology	2008	3,675
38	Dimensionality reduction for visualizing single-cell data using UMAP	Nature Biotechnology	2019	3,600
39	Nanoscale Memristor Device as Synapse in Neuromorphic Systems	Nano Letters	2010	3,597
40	Nonlinear magic: multiphoton microscopy in the biosciences	Nature Biotechnology	2003	3,583
41	Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies	Nature Biotechnology	2006	3,578
42	SignalP 5.0 improves signal peptide predictions using deep neural networks	Nature Biotechnology	2019	3,566
43	Photoluminescence from Chemically Exfoliated MoS₂	Nano Letters	2011	3,497
44	A clearer vision for in vivo imaging	Nature Biotechnology	2001	3,459
45	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9	Nature Biotechnology	2016	3,430
46	Commercial applications of microalgae	Journal of Bioscience and Bioengineering	2006	3,415
47	What is flux balance analysis?	Nature Biotechnology	2010	3,369
48	Differential analysis of gene regulation at transcript resolution with RNA-seq	Nature Biotechnology	2013	3,337
49	Probing the Cytotoxicity of Semiconductor Quantum Dots	Nano Letters	2004	3,191
50	Assembly of long, error-prone reads using repeat graphs	Nature Biotechnology	2019	3,190
51	A Joint Coordinate System for the Clinical Description of Three-Dimensional Motions: Application to the Knee	Journal of Biomechanical Engineering	1983	3,153
52	Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling	Nature Biotechnology	2009	3,144
53	An economic method for the fluorescent labeling of PCR fragments	Nature Biotechnology	2000	3,137
54	PICRUSt2 for prediction of metagenome functions	Nature Biotechnology	2020	3,120
55	Fabrication of novel biomaterials through molecular self-assembly	Nature Biotechnology	2003	3,081
56	Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking	Nature Biotechnology	2016	3,065
57	Expression monitoring by hybridization to high-density oligonucleotide arrays	Nature Biotechnology	1996	3,057
58	A cross-platform toolkit for mass spectrometry and proteomics	Nature Biotechnology	2012	3,018
59	What is a support vector machine?	Nature Biotechnology	2006	2,963
60	Quantifying Defects in Graphene via Raman Spectroscopy at Different Excitation Energies	Nano Letters	2011	2,939
61	Graphene-Based Supercapacitor with an Ultrahigh Energy Density	Nano Letters	2010	2,915
62	Synthesis of N-Doped Graphene by Chemical Vapor Deposition and Its Electrical Properties	Nano Letters	2009	2,873
63	The use of nanocrystals in biological detection	Nature Biotechnology	2004	2,869
64	Cyclodextrins and their uses: a review	Process Biochemistry	2004	2,861
65	Perspectives on Carbon Nanotubes and Graphene Raman Spectroscopy	Nano Letters	2010	2,847
66	Transfer of Large-Area Graphene Films for High-Performance Transparent Conductive Electrodes	Nano Letters	2009	2,845
67	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells	Nature Biotechnology	2013	2,843
68	A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life	Nature Biotechnology	2018	2,798
69	Large Reversible Li Storage of Graphene Nanosheet Families for Use in Rechargeable Lithium Ion Batteries	Nano Letters	2008	2,740
70	CRISPR-Cas systems for editing, regulating and targeting genomes	Nature Biotechnology	2014	2,726
71	Efficient genome editing in zebrafish using a CRISPR-Cas system	Nature Biotechnology	2013	2,712
72	Determining cell type abundance and expression from bulk tissues with digital cytometry	Nature Biotechnology	2019	2,701
73	Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor	Biotechnology and Bioengineering	2009	2,664
74	Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts	Nature Biotechnology	2008	2,614
75	Impermeable Atomic Membranes from Graphene Sheets	Nano Letters	2008	2,611
76	Synthesis of Water Soluble Graphene	Nano Letters	2008	2,594
77	Microfluidic organs-on-chips	Nature Biotechnology	2014	2,578
78	Infrared Perfect Absorber and Its Application As Plasmonic Sensor	Nano Letters	2010	2,572
79	ProteomeXchange provides globally coordinated proteomics data submission and dissemination	Nature Biotechnology	2014	2,572
80	Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro	Nature Biotechnology	2000	2,569
81	A generic protein purification method for protein complex characterization and proteome exploration	Nature Biotechnology	1999	2,558
82	Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules	Nature Biotechnology	2001	2,554
83	A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications	Nature Biotechnology	2002	2,553
84	Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots	Nature Biotechnology	2003	2,441
85	Graphene Plasmonics: A Platform for Strong Light–Matter Interactions	Nano Letters	2011	2,438
86	Visualizing and interpreting cancer genomics data via the Xena platform	Nature Biotechnology	2020	2,410
87	ZnO Nanowire UV Photodetectors with High Internal Gain	Nano Letters	2007	2,409
88	Hydrogen-Treated TiO₂ Nanowire Arrays for Photoelectrochemical Water Splitting	Nano Letters	2011	2,398
89	Spatially Resolved Raman Spectroscopy of Single- and Few-Layer Graphene	Nano Letters	2007	2,397
90	Large Scale Growth and Characterization of Atomic Hexagonal Boron Nitride Layers	Nano Letters	2010	2,393
91	Epigenetic modifications and human disease	Nature Biotechnology	2010	2,386
92	Predicting the sequence specificities of DNA- and RNA-binding proteins by deep learning	Nature Biotechnology	2015	2,319
93	Hydrogels as extracellular matrix mimics for 3D cell culture	Biotechnology and Bioengineering	2009	2,309
94	Nextflow enables reproducible computational workflows	Nature Biotechnology	2017	2,304
95	The potential and challenges of nanopore sequencing	Nature Biotechnology	2008	2,266
96	Multiplexed electrical detection of cancer markers with nanowire sensor arrays	Nature Biotechnology	2005	2,262
97	MMseqs2 enables sensitive protein sequence searching for the analysis of massive data sets	Nature Biotechnology	2017	2,262
98	Detecting and characterizing circular RNAs	Nature Biotechnology	2014	2,254
99	Graphene in Mice: Ultrahigh In Vivo Tumor Uptake and Efficient Photothermal Therapy	Nano Letters	2010	2,244
100	A quantitative analysis of kinase inhibitor selectivity	Nature Biotechnology	2008	2,223

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