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Top Articles

#TitleJournalYearCitations
1A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry1976215,588
2A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry197692,750
3Basic local alignment search toolJournal of Molecular Biology199082,180
4Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry198758,438
5Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology197535,546
6Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform ExtractionAnalytical Biochemistry198727,111
7A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry198324,572
8Tissue sulfhydryl groupsArchives of Biochemistry and Biophysics195923,177
9Assay for lipid peroxides in animal tissues by thiobarbituric acid reactionAnalytical Biochemistry197922,890
10A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology198222,171
11The calculation of small molecular interactions by the differences of separate total energies. Some procedures with reduced errorsMolecular Physics197020,046
12Measurement of protein using bicinchoninic acidAnalytical Biochemistry198519,774
13The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP AssayAnalytical Biochemistry199616,000
14Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical featuresBiopolymers198313,990
15Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology197713,093
16A logical calculus of the ideas immanent in nervous activityThe Bulletin of Mathematical Biophysics194312,960
17Comparative Protein Modelling by Satisfaction of Spatial RestraintsJournal of Molecular Biology199311,892
18Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDaAnalytical Biochemistry198711,692
19A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology196111,512
20Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. CohenJournal of Molecular Biology200111,404
21Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology198310,861
22Analysis of nitrate, nitrite, and [15N]nitrate in biological fluidsAnalytical Biochemistry198210,819
23Superoxide dismutase: Improved assays and an assay applicable to acrylamide gelsAnalytical Biochemistry197110,405
24A general method applicable to the search for similarities in the amino acid sequence of two proteinsJournal of Molecular Biology19709,639
25The protein data bank: A computer-based archival file for macromolecular structuresJournal of Molecular Biology19778,738
26“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry19818,736
27LIGAND: A versatile computerized approach for characterization of ligand-binding systemsAnalytical Biochemistry19808,732
28A simplification of the protein assay method of Lowry et al. which is more generally applicableAnalytical Biochemistry19778,587
29Inference of Macromolecular Assemblies from Crystalline StateJournal of Molecular Biology20078,484
30On the nature of allosteric transitions: A plausible modelJournal of Molecular Biology19658,467
31Identification of common molecular subsequencesJournal of Molecular Biology19818,440
32Photoperoxidation in isolated chloroplastsArchives of Biochemistry and Biophysics19688,296
33Solvent content of protein crystalsJournal of Molecular Biology19688,228
34A molecular dynamics method for simulations in the canonical ensembleMolecular Physics19848,175
35A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counterAnalytical Biochemistry19607,552
36How useful is SBF in predicting in vivo bone bioactivity?Biomaterials20067,510
37The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescenceBiochimica Et Biophysica Acta - General Subjects19897,061
38Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based TherapiesTissue Engineering20016,976
39Standard methods: For the examination of water and waste waterAnalytical Biochemistry19906,705
40A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samplesAnalytical Biochemistry19786,578
41Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagentAnalytical Biochemistry19686,535
42New method for quantitative determination of uronic acidsAnalytical Biochemistry19736,487
43T-coffee: a novel method for fast and accurate multiple sequence alignment 1 1Edited by J. ThorntonJournal of Molecular Biology20006,404
44A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry19846,353
45Selective extraction of polyoma DNA from infected mouse cell culturesJournal of Molecular Biology19676,287
46A modified uronic acid carbazole reactionAnalytical Biochemistry19626,215
47Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesJournal of Molecular Biology19866,199
48Improved Prediction of Signal Peptides: SignalP 3.0Journal of Molecular Biology20046,015
49Genetic regulatory mechanisms in the synthesis of proteinsJournal of Molecular Biology19616,014
50Synthesis and surface engineering of iron oxide nanoparticles for biomedical applicationsBiomaterials20055,951
51Development and validation of a genetic algorithm for flexible docking 1 1Edited by F. E. CohenJournal of Molecular Biology19975,937
52The interpretation of protein structures: Estimation of static accessibilityJournal of Molecular Biology19715,785
53Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissuesAnalytical Biochemistry19695,750
54Calculation of protein extinction coefficients from amino acid sequence dataAnalytical Biochemistry19895,457
55SCOP: a structural classification of proteins database for the investigation of sequences and structuresJournal of Molecular Biology19955,400
56Porosity of 3D biomaterial scaffolds and osteogenesisBiomaterials20055,351
57Determination of protein: A modification of the lowry method that gives a linear photometric responseAnalytical Biochemistry19725,314
58MR diffusion tensor spectroscopy and imagingBiophysical Journal19945,262
59Universal chemical assay for the detection and determination of siderophoresAnalytical Biochemistry19875,170
60On the size of the active site in proteases. I. PapainBiochemical and Biophysical Research Communications19675,135
613D Slicer as an image computing platform for the Quantitative Imaging NetworkMagnetic Resonance Imaging20125,126
62A simple, rapid, and sensitive DNA assay procedureAnalytical Biochemistry19805,106
63EasySpin, a comprehensive software package for spectral simulation and analysis in EPRJournal of Magnetic Resonance20065,100
64Protein secondary structure prediction based on position-specific scoring matrices 1 1Edited by G. Von HeijneJournal of Molecular Biology19995,026
65Impulses and Physiological States in Theoretical Models of Nerve MembraneBiophysical Journal19614,953
66Alzheimer's disease: Initial report of the purification and characterization of a novel cerebrovascular amyloid proteinBiochemical and Biophysical Research Communications19844,858
67Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopyBiochimica Et Biophysica Acta - Bioenergetics19894,780
68Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications19784,728
69Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteinsJournal of Molecular Biology19784,696
70Development of recommendations for SEMG sensors and sensor placement proceduresJournal of Electromyography and Kinesiology20004,650
71Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodiesAnalytical Biochemistry19664,462
72Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperatureJournal of Molecular Biology19624,433
73A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications19664,414
74Hydrogels for tissue engineering: scaffold design variables and applicationsBiomaterials20034,376
75Scaffolds in tissue engineering bone and cartilageBiomaterials20004,353
76Determination of malonaldehyde precursor in tissues by thiobarbituric acid testAnalytical Biochemistry19784,352
77The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytesArchives of Biochemistry and Biophysics19634,329
78Paradoxical Decrease of an Adipose-Specific Protein, Adiponectin, in ObesityBiochemical and Biophysical Research Communications19994,315
79Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivityAnalytical Biochemistry19814,299
80A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology19694,258
81A highly sensitive adenylate cyclase assayAnalytical Biochemistry19744,186
82Predicting Subcellular Localization of Proteins Based on their N-terminal Amino Acid SequenceJournal of Molecular Biology20004,166
83Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridineAnalytical Biochemistry19804,155
84Maturation of the head of bacteriophage T4Journal of Molecular Biology19734,042
85Self-consistent perturbation theory of diamagnetismMolecular Physics19744,026
86Colorimetric assay of catalaseAnalytical Biochemistry19724,020
87Diffusion of univalent ions across the lamellae of swollen phospholipidsJournal of Molecular Biology19654,013
88Theoretical studies of enzymic reactions: Dielectric, electrostatic and steric stabilization of the carbonium ion in the reaction of lysozymeJournal of Molecular Biology19763,971
89SCOP: A structural classification of proteins database for the investigation of sequences and structuresJournal of Molecular Biology19953,952
90A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipidsAnalytical Biochemistry19843,890
91Protein Structure Comparison by Alignment of Distance MatricesJournal of Molecular Biology19933,851
92Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gelsBiochemical and Biophysical Research Communications19673,848
93Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin EAnalytical Biochemistry19993,789
94Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology19803,786
95Magnesium and its alloys as orthopedic biomaterials: A reviewBiomaterials20063,782
96A fluorometric method for determination of oxidized and reduced glutathione in tissuesAnalytical Biochemistry19763,770
97Prediction of complete gene structures in human genomic DNAJournal of Molecular Biology19973,746
98The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acidArchives of Biochemistry and Biophysics19613,643
99Silver staining of proteins in polyacrylamide gelsAnalytical Biochemistry19813,577
100A rapid boiling method for the preparation of bacterial plasmidsAnalytical Biochemistry19813,577