# | Title | Journal | Year | Citations |
---|
1 | A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding | Analytical Biochemistry | 1976 | 215,588 |
2 | A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding | Analytical Biochemistry | 1976 | 92,750 |
3 | Basic local alignment search tool | Journal of Molecular Biology | 1990 | 82,180 |
4 | Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction | Analytical Biochemistry | 1987 | 58,438 |
5 | Detection of specific sequences among DNA fragments separated by gel electrophoresis | Journal of Molecular Biology | 1975 | 35,546 |
6 | Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction | Analytical Biochemistry | 1987 | 27,111 |
7 | A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity | Analytical Biochemistry | 1983 | 24,572 |
8 | Tissue sulfhydryl groups | Archives of Biochemistry and Biophysics | 1959 | 23,177 |
9 | Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction | Analytical Biochemistry | 1979 | 22,890 |
10 | A simple method for displaying the hydropathic character of a protein | Journal of Molecular Biology | 1982 | 22,171 |
11 | The calculation of small molecular interactions by the differences of separate total energies. Some procedures with reduced errors | Molecular Physics | 1970 | 20,046 |
12 | Measurement of protein using bicinchoninic acid | Analytical Biochemistry | 1985 | 19,774 |
13 | The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay | Analytical Biochemistry | 1996 | 16,000 |
14 | Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical features | Biopolymers | 1983 | 13,990 |
15 | Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I | Journal of Molecular Biology | 1977 | 13,093 |
16 | A logical calculus of the ideas immanent in nervous activity | The Bulletin of Mathematical Biophysics | 1943 | 12,960 |
17 | Comparative Protein Modelling by Satisfaction of Spatial Restraints | Journal of Molecular Biology | 1993 | 11,892 |
18 | Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa | Analytical Biochemistry | 1987 | 11,692 |
19 | A procedure for the isolation of deoxyribonucleic acid from micro-organisms | Journal of Molecular Biology | 1961 | 11,512 |
20 | Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen | Journal of Molecular Biology | 2001 | 11,404 |
21 | Studies on transformation of Escherichia coli with plasmids | Journal of Molecular Biology | 1983 | 10,861 |
22 | Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids | Analytical Biochemistry | 1982 | 10,819 |
23 | Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels | Analytical Biochemistry | 1971 | 10,405 |
24 | A general method applicable to the search for similarities in the amino acid sequence of two proteins | Journal of Molecular Biology | 1970 | 9,639 |
25 | The protein data bank: A computer-based archival file for macromolecular structures | Journal of Molecular Biology | 1977 | 8,738 |
26 | “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A | Analytical Biochemistry | 1981 | 8,736 |
27 | LIGAND: A versatile computerized approach for characterization of ligand-binding systems | Analytical Biochemistry | 1980 | 8,732 |
28 | A simplification of the protein assay method of Lowry et al. which is more generally applicable | Analytical Biochemistry | 1977 | 8,587 |
29 | Inference of Macromolecular Assemblies from Crystalline State | Journal of Molecular Biology | 2007 | 8,484 |
30 | On the nature of allosteric transitions: A plausible model | Journal of Molecular Biology | 1965 | 8,467 |
31 | Identification of common molecular subsequences | Journal of Molecular Biology | 1981 | 8,440 |
32 | Photoperoxidation in isolated chloroplasts | Archives of Biochemistry and Biophysics | 1968 | 8,296 |
33 | Solvent content of protein crystals | Journal of Molecular Biology | 1968 | 8,228 |
34 | A molecular dynamics method for simulations in the canonical ensemble | Molecular Physics | 1984 | 8,175 |
35 | A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter | Analytical Biochemistry | 1960 | 7,552 |
36 | How useful is SBF in predicting in vivo bone bioactivity? | Biomaterials | 2006 | 7,510 |
37 | The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence | Biochimica Et Biophysica Acta - General Subjects | 1989 | 7,061 |
38 | Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies | Tissue Engineering | 2001 | 6,976 |
39 | Standard methods: For the examination of water and waste water | Analytical Biochemistry | 1990 | 6,705 |
40 | A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples | Analytical Biochemistry | 1978 | 6,578 |
41 | Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent | Analytical Biochemistry | 1968 | 6,535 |
42 | New method for quantitative determination of uronic acids | Analytical Biochemistry | 1973 | 6,487 |
43 | T-coffee: a novel method for fast and accurate multiple sequence alignment 1 1Edited by J. Thornton | Journal of Molecular Biology | 2000 | 6,404 |
44 | A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity | Analytical Biochemistry | 1984 | 6,353 |
45 | Selective extraction of polyoma DNA from infected mouse cell cultures | Journal of Molecular Biology | 1967 | 6,287 |
46 | A modified uronic acid carbazole reaction | Analytical Biochemistry | 1962 | 6,215 |
47 | Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes | Journal of Molecular Biology | 1986 | 6,199 |
48 | Improved Prediction of Signal Peptides: SignalP 3.0 | Journal of Molecular Biology | 2004 | 6,015 |
49 | Genetic regulatory mechanisms in the synthesis of proteins | Journal of Molecular Biology | 1961 | 6,014 |
50 | Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications | Biomaterials | 2005 | 5,951 |
51 | Development and validation of a genetic algorithm for flexible docking 1 1Edited by F. E. Cohen | Journal of Molecular Biology | 1997 | 5,937 |
52 | The interpretation of protein structures: Estimation of static accessibility | Journal of Molecular Biology | 1971 | 5,785 |
53 | Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues | Analytical Biochemistry | 1969 | 5,750 |
54 | Calculation of protein extinction coefficients from amino acid sequence data | Analytical Biochemistry | 1989 | 5,457 |
55 | SCOP: a structural classification of proteins database for the investigation of sequences and structures | Journal of Molecular Biology | 1995 | 5,400 |
56 | Porosity of 3D biomaterial scaffolds and osteogenesis | Biomaterials | 2005 | 5,351 |
57 | Determination of protein: A modification of the lowry method that gives a linear photometric response | Analytical Biochemistry | 1972 | 5,314 |
58 | MR diffusion tensor spectroscopy and imaging | Biophysical Journal | 1994 | 5,262 |
59 | Universal chemical assay for the detection and determination of siderophores | Analytical Biochemistry | 1987 | 5,170 |
60 | On the size of the active site in proteases. I. Papain | Biochemical and Biophysical Research Communications | 1967 | 5,135 |
61 | 3D Slicer as an image computing platform for the Quantitative Imaging Network | Magnetic Resonance Imaging | 2012 | 5,126 |
62 | A simple, rapid, and sensitive DNA assay procedure | Analytical Biochemistry | 1980 | 5,106 |
63 | EasySpin, a comprehensive software package for spectral simulation and analysis in EPR | Journal of Magnetic Resonance | 2006 | 5,100 |
64 | Protein secondary structure prediction based on position-specific scoring matrices 1 1Edited by G. Von Heijne | Journal of Molecular Biology | 1999 | 5,026 |
65 | Impulses and Physiological States in Theoretical Models of Nerve Membrane | Biophysical Journal | 1961 | 4,953 |
66 | Alzheimer's disease: Initial report of the purification and characterization of a novel cerebrovascular amyloid protein | Biochemical and Biophysical Research Communications | 1984 | 4,858 |
67 | Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy | Biochimica Et Biophysica Acta - Bioenergetics | 1989 | 4,780 |
68 | Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril | Biochemical and Biophysical Research Communications | 1978 | 4,728 |
69 | Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins | Journal of Molecular Biology | 1978 | 4,696 |
70 | Development of recommendations for SEMG sensors and sensor placement procedures | Journal of Electromyography and Kinesiology | 2000 | 4,650 |
71 | Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies | Analytical Biochemistry | 1966 | 4,462 |
72 | Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature | Journal of Molecular Biology | 1962 | 4,433 |
73 | A membrane-filter technique for the detection of complementary DNA | Biochemical and Biophysical Research Communications | 1966 | 4,414 |
74 | Hydrogels for tissue engineering: scaffold design variables and applications | Biomaterials | 2003 | 4,376 |
75 | Scaffolds in tissue engineering bone and cartilage | Biomaterials | 2000 | 4,353 |
76 | Determination of malonaldehyde precursor in tissues by thiobarbituric acid test | Analytical Biochemistry | 1978 | 4,352 |
77 | The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes | Archives of Biochemistry and Biophysics | 1963 | 4,329 |
78 | Paradoxical Decrease of an Adipose-Specific Protein, Adiponectin, in Obesity | Biochemical and Biophysical Research Communications | 1999 | 4,315 |
79 | Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity | Analytical Biochemistry | 1981 | 4,299 |
80 | A complementation analysis of the restriction and modification of DNA in Escherichia coli | Journal of Molecular Biology | 1969 | 4,258 |
81 | A highly sensitive adenylate cyclase assay | Analytical Biochemistry | 1974 | 4,186 |
82 | Predicting Subcellular Localization of Proteins Based on their N-terminal Amino Acid Sequence | Journal of Molecular Biology | 2000 | 4,166 |
83 | Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine | Analytical Biochemistry | 1980 | 4,155 |
84 | Maturation of the head of bacteriophage T4 | Journal of Molecular Biology | 1973 | 4,042 |
85 | Self-consistent perturbation theory of diamagnetism | Molecular Physics | 1974 | 4,026 |
86 | Colorimetric assay of catalase | Analytical Biochemistry | 1972 | 4,020 |
87 | Diffusion of univalent ions across the lamellae of swollen phospholipids | Journal of Molecular Biology | 1965 | 4,013 |
88 | Theoretical studies of enzymic reactions: Dielectric, electrostatic and steric stabilization of the carbonium ion in the reaction of lysozyme | Journal of Molecular Biology | 1976 | 3,971 |
89 | SCOP: A structural classification of proteins database for the investigation of sequences and structures | Journal of Molecular Biology | 1995 | 3,952 |
90 | A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids | Analytical Biochemistry | 1984 | 3,890 |
91 | Protein Structure Comparison by Alignment of Distance Matrices | Journal of Molecular Biology | 1993 | 3,851 |
92 | Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels | Biochemical and Biophysical Research Communications | 1967 | 3,848 |
93 | Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E | Analytical Biochemistry | 1999 | 3,789 |
94 | Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing | Journal of Molecular Biology | 1980 | 3,786 |
95 | Magnesium and its alloys as orthopedic biomaterials: A review | Biomaterials | 2006 | 3,782 |
96 | A fluorometric method for determination of oxidized and reduced glutathione in tissues | Analytical Biochemistry | 1976 | 3,770 |
97 | Prediction of complete gene structures in human genomic DNA | Journal of Molecular Biology | 1997 | 3,746 |
98 | The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid | Archives of Biochemistry and Biophysics | 1961 | 3,643 |
99 | Silver staining of proteins in polyacrylamide gels | Analytical Biochemistry | 1981 | 3,577 |
100 | A rapid boiling method for the preparation of bacterial plasmids | Analytical Biochemistry | 1981 | 3,577 |