| # | Title | Journal | Year | Citations |
|---|
|
| 1 | Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors | Gene | 1985 | 16,642 |
| 2 | Site-directed mutagenesis by overlap extension using the polymerase chain reaction | Gene | 1989 | 7,982 |
| 3 | The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers | Gene | 1982 | 6,767 |
| 4 | A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria | Bio/technology | 1983 | 6,593 |
| 5 | Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase | Gene | 1988 | 6,320 |
| 6 | Construction and characterization of new cloning vehicle. II. A multipurpose cloning system | Gene | 1977 | 5,197 |
| 7 | Efficient selection for high-expression transfectants with a novel eukaryotic vector | Gene | 1991 | 4,955 |
| 8 | A simple and very efficient method for generating cDNA libraries | Gene | 1983 | 4,907 |
| 9 | Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing | Gene | 1984 | 4,620 |
| 10 | CLUSTAL: a package for performing multiple sequence alignment on a microcomputer | Gene | 1988 | 3,467 |
| 11 | A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments | Gene | 1982 | 3,300 |
| 12 | ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering | Trends in Biotechnology | 2013 | 3,219 |
| 13 | Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes | Gene | 1995 | 3,154 |
| 14 | Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension | Gene | 1989 | 3,004 |
| 15 | New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites | Gene | 1988 | 2,939 |
| 16 | Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose Tissue | Stem Cells | 2006 | 2,911 |
| 17 | FACS-optimized mutants of the green fluorescent protein (GFP) | Gene | 1996 | 2,862 |
| 18 | Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis | Gene | 1983 | 2,698 |
| 19 | The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions | Gene | 1988 | 2,672 |
| 20 | Proteobacteria: microbial signature of dysbiosis in gut microbiota | Trends in Biotechnology | 2015 | 2,669 |
| 21 | A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli | Gene | 1987 | 2,631 |
| 22 | Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum | Gene | 1994 | 2,595 |
| 23 | Antibacterial properties of nanoparticles | Trends in Biotechnology | 2012 | 2,192 |
| 24 | Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing | Stem Cells | 2007 | 2,132 |
| 25 | The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis | Molecular Breeding | 1996 | 2,031 |
| 26 | Primary structure of the Aequorea victoria green-fluorescent protein | Gene | 1992 | 1,993 |
| 27 | The ‘effective number of codons’ used in a gene | Gene | 1990 | 1,980 |
| 28 | High efficiency transformation of Escherichia coli with plasmids | Gene | 1990 | 1,884 |
| 29 | Microbial fuel cells: novel biotechnology for energy generation | Trends in Biotechnology | 2005 | 1,880 |
| 30 | Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds | Gene | 1995 | 1,827 |
| 31 | A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants | Gene | 1998 | 1,819 |
| 32 | Recent advances in bone tissue engineering scaffolds | Trends in Biotechnology | 2012 | 1,810 |
| 33 | In vitro insertional mutagenesis with a selectable DNA fragment | Gene | 1984 | 1,781 |
| 34 | Epidermal growth factor receptor (EGFR) signaling in cancer | Gene | 2006 | 1,781 |
| 35 | Bone Marrow Stromal Stem Cells: Nature, Biology, and Potential Applications | Stem Cells | 2001 | 1,777 |
| 36 | Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant | Gene | 1995 | 1,745 |
| 37 | Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair—Current Views | Stem Cells | 2007 | 1,741 |
| 38 | Biodiesel from microalgae beats bioethanol | Trends in Biotechnology | 2008 | 1,725 |
| 39 | Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction | Biotechnology Annual Review | 2005 | 1,695 |
| 40 | Multifunctional yeast high-copy-number shuttle vectors | Gene | 1992 | 1,675 |
| 41 | Soft robotics: a bioinspired evolution in robotics | Trends in Biotechnology | 2013 | 1,669 |
| 42 | Alginate as immobilization matrix for cells | Trends in Biotechnology | 1990 | 1,648 |
| 43 | Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans | Gene | 2001 | 1,643 |
| 44 | Improved single and multicopy lac-based cloning vectors for protein and operon fusions | Gene | 1987 | 1,622 |
| 45 | Vectors for selective expression of cloned DNAs by T7 RNA polymerase | Gene | 1987 | 1,599 |
| 46 | Genic microsatellite markers in plants: features and applications | Trends in Biotechnology | 2005 | 1,568 |
| 47 | Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria | Gene | 1988 | 1,560 |
| 48 | Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells | Gene | 1979 | 1,540 |
| 49 | Mesenchymal Stem Cells Enhance Wound Healing Through Differentiation and Angiogenesis | Stem Cells | 2007 | 1,496 |
| 50 | Acetylation and deacetylation of non-histone proteins | Gene | 2005 | 1,495 |
| 51 | Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF) | Trends in Biotechnology | 2009 | 1,420 |
| 52 | Graphene and graphene oxide: biofunctionalization and applications in biotechnology | Trends in Biotechnology | 2011 | 1,348 |
| 53 | Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. | Gene | 1992 | 1,320 |
| 54 | The expanding scope of antimicrobial peptide structures and their modes of action | Trends in Biotechnology | 2011 | 1,289 |
| 55 | A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector | Gene | 1987 | 1,277 |
| 56 | Genetics and biology of vitamin D receptor polymorphisms | Gene | 2004 | 1,276 |
| 57 | Initiation of translation in prokaryotes and eukaryotes | Gene | 1999 | 1,272 |
| 58 | Haematococcus astaxanthin: applications for human health and nutrition | Trends in Biotechnology | 2003 | 1,264 |
| 59 | Concise Review: MSC-Derived Exosomes for Cell-Free Therapy | Stem Cells | 2017 | 1,248 |
| 60 | Bio-ethanol – the fuel of tomorrow from the residues of today | Trends in Biotechnology | 2006 | 1,247 |
| 61 | Nanosilver as a new generation of nanoproduct in biomedical applications | Trends in Biotechnology | 2010 | 1,243 |
| 62 | Cationic peptides: a new source of antibiotics | Trends in Biotechnology | 1998 | 1,241 |
| 63 | Role for Interferon-γ in the Immunomodulatory Activity of Human Bone Marrow Mesenchymal Stem Cells | Stem Cells | 2006 | 1,240 |
| 64 | Biological Synthesis of Nanoparticles from Plants and Microorganisms | Trends in Biotechnology | 2016 | 1,237 |
| 65 | SELEX—A (r)evolutionary method to generate high-affinity nucleic acid ligands | New Biotechnology | 2007 | 1,223 |
| 66 | Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord | Stem Cells | 2004 | 1,220 |
| 67 | Plasmid screening at high colony density | Gene | 1980 | 1,213 |
| 68 | Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene | Gene | 1979 | 1,211 |
| 69 | Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas | Gene | 1981 | 1,195 |
| 70 | The essence of SNPs | Gene | 1999 | 1,177 |
| 71 | Metabolomics by numbers: acquiring and understanding global metabolite data | Trends in Biotechnology | 2004 | 1,165 |
| 72 | Mesenchymal Stem Cells Can Be Differentiated Into Endothelial Cells In Vitro | Stem Cells | 2004 | 1,160 |
| 73 | Organ printing: computer-aided jet-based 3D tissue engineering | Trends in Biotechnology | 2003 | 1,149 |
| 74 | Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli | Gene | 1991 | 1,114 |
| 75 | Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector | Gene | 1986 | 1,096 |
| 76 | Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments | Gene | 1979 | 1,087 |
| 77 | Systematic functional analysis of the yeast genome | Trends in Biotechnology | 1998 | 1,076 |
| 78 | Laccases: blue enzymes for green chemistry | Trends in Biotechnology | 2006 | 1,076 |
| 79 | Codon bias and heterologous protein expression | Trends in Biotechnology | 2004 | 1,072 |
| 80 | A simple phase-extraction assay for chloramphenicol acyltransferase activity | Gene | 1988 | 1,069 |
| 81 | Towards practical implementation of bioelectrochemical wastewater treatment | Trends in Biotechnology | 2008 | 1,059 |
| 82 | Isolation of Mesenchymal Stem Cells of Fetal or Maternal Origin from Human Placenta | Stem Cells | 2004 | 1,058 |
| 83 | Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli | Gene | 1988 | 1,051 |
| 84 | Vascularization in tissue engineering | Trends in Biotechnology | 2008 | 1,049 |
| 85 | Thiamine-repressible expression vectors pREP and pRIP for fission yeast | Gene | 1993 | 1,041 |
| 86 | The role of bioreactors in tissue engineering | Trends in Biotechnology | 2004 | 1,037 |
| 87 | Construction and characterization of new cloning vehicles I. Ampicillin-resistant derivatives of the plasmid pMB9 | Gene | 1977 | 1,036 |
| 88 | The Sp-family of transcription factors | Gene | 1999 | 1,030 |
| 89 | Function of alternative splicing | Gene | 2005 | 1,030 |
| 90 | Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions | Gene | 1990 | 1,019 |
| 91 | Immunophenotype of Human Adipose-Derived Cells: Temporal Changes in Stromal-Associated and Stem Cell–Associated Markers | Stem Cells | 2006 | 1,015 |
| 92 | Signaling through the JAK/STAT pathway, recent advances and future challenges | Gene | 2002 | 1,007 |
| 93 | Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems | Trends in Biotechnology | 2004 | 1,002 |
| 94 | Progress with Proteome Projects: Why all Proteins Expressed by a Genome Should be Identified and How To Do It | Biotechnology and Genetic Engineering Reviews | 1996 | 1,000 |
| 95 | Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5 | Gene | 1982 | 997 |
| 96 | Commercial potential for Haematococcus microalgae as a natural source of astaxanthin | Trends in Biotechnology | 2000 | 994 |
| 97 | Concise Review: Adipose Tissue-Derived Stromal Cells—Basic and Clinical Implications for Novel Cell-Based Therapies | Stem Cells | 2007 | 982 |
| 98 | Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria | Gene | 1993 | 978 |
| 99 | Non-invasive wearable electrochemical sensors: a review | Trends in Biotechnology | 2014 | 972 |
| 100 | Potential of siderophore-producing bacteria for improving heavy metal phytoextraction | Trends in Biotechnology | 2010 | 969 |
