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Immunohistochemical Assessment of BAP1 Protein in Mucoepidermoid Carcinomas

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Indian Journal of Otolaryngology and Head & Neck Surgery Aims and scope Submit manuscript

Abstract

Mucoepidermoid carcinomas are common malignant salivary gland tumors. Despite recent advances in diagnosis and treatment, there has not been much improvement in outcome of these patients, necessitating identification of novel targeted therapeutic agents. Genomic profiling of mucoepidermoid carcinomas has recently revealed aberrations in BAP1 gene. Therefore, we conducted this study to identify BAP1 loss by immunohistochemistry in these tumors. Mucoepidermoid carcinoma cases were retrieved; hematoxylin-and-eosin stained sections were reviewed. Immunohistochemistry for BAP1 was performed. Forty cases were assessed, including 25 salivary gland and 15 pulmonary mucoepidermoid carcinomas. There were 19 cases in the parotid (76%), two in submandibular gland (8%), and remaining 16% from minor salivary gland locations. Ten (40%) were low grade, nine (36%) were intermediate grade, and six (24%) were high grade mucoepidermoid carcinomas. Thirteen (86.7%) pulmonary mucoepidermoid carcinomas were tracheobronchial, while two (13.3%) were intraparenchymal; all were low grade mucoepidermoid carcinomas. On immunohistochemistry, BAP1 nuclear staining was retained in all cases (100%), irrespective of tumor location or grade. Therapeutic connotations necessitate the identification of readily applicable techniques to detect BAP1 loss in mucoepidermoid carcinomas. Using immunohistochemistry, loss of BAP1 staining was not seen in any of our cases, suggesting insensitivity of BAP1 IHC to detect aberrations at genomic level in these tumors. Analysis of BAP1 alterations by targeted sequencing may therefore be performed prior to excluding the possibility of response to BAP1-targeted therapeutics based on immunohistochemistry alone.

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Authors and Affiliations

  1. Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India

    Aanchal Kakkar, Prerna Guleria & Deepali Jain

  2. Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, 110029, India

    Karan Madan

  3. Department of Otorhinolaryngology and Head and Neck Surgery, All India Institute of Medical Sciences, New Delhi, 110029, India

    Rajeev Kumar

  4. Department of Surgical Oncology, All India Institute of Medical Sciences, New Delhi, 110029, India

    Sunil Kumar

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  1. Aanchal Kakkar
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  2. Prerna Guleria
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  3. Karan Madan
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  4. Rajeev Kumar
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  5. Sunil Kumar
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  6. Deepali Jain
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Correspondence to Deepali Jain.

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Kakkar, A., Guleria, P., Madan, K. et al. Immunohistochemical Assessment of BAP1 Protein in Mucoepidermoid Carcinomas. Indian J Otolaryngol Head Neck Surg 71, 33–37 (2019). https://doi.org/10.1007/s12070-018-1549-3

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  • DOI: https://doi.org/10.1007/s12070-018-1549-3

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