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Evaluation of androgenic competence through anther culture in common eggplant and related species

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Abstract

Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant (Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 hybrid seed of some commercial varieties. However, a comprehensive study of the variation of this useful trait among different materials of common eggplant and related species is still lacking. In this work, we studied the androgenic response of 12 accessions of common eggplant and related materials from the primary (eggplant complex) and secondary genepools. We cultured anthers of all the accessions under the same experimental conditions, and studied their competence to produce calli, embryos and plants, as well as the quality and origin of the embryos produced. In our conditions, anthers of 11 out of the 12 accessions produced somatic calli, whereas only 5 also produced microspore-derived embryos, with variable results in terms of embryo quality and of frequency of embryo induction and plant germination. Embryos of responding accessions were initially haploid, and reached the DH status, verified with SSR markers, after a defined period of culture. In addition to other aspects common to many androgenesis-responsive species, our results allowed us to extract conclusions particular to common eggplant and relatives, including the difficulty for finding sources of androgenic competence out of S. melongena, the reduced impact of calli in the production of non-DH individuals, and the need to avoid the occurrence of severe anatomical and functional problems in the apex of most embryos, which seriously reduces their germinative success.

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Acknowledgments

We acknowledge Drs. Begoña Renau and Antonio Serrano, and Mrs Nuria Palacios for their excellent technical work, as well as the staff of the COMAV greenhouses for their valuable help. Thanks are also due to Dr. Santiago Vilanova for providing us with the SSRs used in this work. This work was supported by grants from Spanish MICINN AGL2006-06678 and AGL2010-17895 to JMSS, and AGL2009-07257 to JP.

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  1. COMAV—Universitat Politècnica de València, CPI, Edificio 8E—Escalera I, Camino de Vera, s/n, 46022, Valencia, Spain

    Pablo Salas, Jaime Prohens & José M. Seguí-Simarro

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  1. Pablo Salas
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  2. Jaime Prohens
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  3. José M. Seguí-Simarro
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Correspondence to José M. Seguí-Simarro.

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Salas, P., Prohens, J. & Seguí-Simarro, J.M. Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica 182, 261–274 (2011). https://doi.org/10.1007/s10681-011-0490-2

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