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Characterization of Plant Growth–Promoting Traits of Bacteria Isolated from Larval Guts of Diamondback Moth Plutella xylostella (Lepidoptera: Plutellidae)

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Abstract

Eight bacterial isolates from the larval guts of Diamondback moths (Plutella xylostella) were tested for their plant growth–promoting (PGP) traits and effects on early plant growth. All of the strains tested positive for nitrogen fixation and indole 3-acetic acid (IAA) and salicylic acid production but negative for hydrogen cyanide and pectinase production. In addition, five of the isolates exhibited significant levels of tricalcium phosphate and zinc oxide solubilization; six isolates were able to oxidize sulfur in growth media; and four isolates tested positive for chitinase and β-1,3-glucanase activities. Based on their IAA production, six strains including four that were 1-aminocyclopropane-1-carboxylate (ACC) deaminase positive and two that were ACC deaminase negative were tested for PGP activity on the early growth of canola and tomato seeds under gnotobiotic conditions. Acinetobacter sp. PSGB04 significantly increased root length (41%), seedling vigor, and dry biomass (30%) of the canola test plants, whereas Pseudomonas sp. PRGB06 inhibited the mycelial growth of Botrytis cinerea, Colletotrichum coccodes, C. gleospoiroides, Rhizoctonia solani, and Sclerotia sclerotiorum under in vitro conditions. A significant increase, greater than that of the control, was also noted for growth parameters of the tomato test plants when the seeds were treated with PRGB06. Therefore, the results of the present study suggest that bacteria associated with insect larval guts possess PGP traits and positively influence plant growth. Therefore, insect gut bacteria as effective PGP agents represent an unexplored niche and may broaden the spectrum of beneficial bacteria available for crop production.

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Acknowledgments

The authors acknowledge the financial support given by a BK21 Research Fellowship through a doctoral fellowship to P. Indiragandhi and the Agricultural Research Promotion Center (Republic of Korea). Also, thanks are due to Dr. V. S. Saravanan and Dr. P. Trivedi for their critical reading of this manuscript.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju, 361763, Republic of Korea

    P. Indiragandhi, R. Anandham, M. Madhaiyan & T. M. Sa

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  1. P. Indiragandhi
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  2. R. Anandham
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  3. M. Madhaiyan
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  4. T. M. Sa
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Correspondence to T. M. Sa.

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Indiragandhi, P., Anandham, R., Madhaiyan, M. et al. Characterization of Plant Growth–Promoting Traits of Bacteria Isolated from Larval Guts of Diamondback Moth Plutella xylostella (Lepidoptera: Plutellidae). Curr Microbiol 56, 327–333 (2008). https://doi.org/10.1007/s00284-007-9086-4

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  • DOI: https://doi.org/10.1007/s00284-007-9086-4

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