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Magnesium Deficiency Enhances Hydrogen Peroxide Production and Oxidative Damage in Chick Embryo Hepatocyte In Vitro

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Abstract

Magnesium deficiency and oxidative stress have been identified as correlative factors in many diseases. The origin of free radicals correlated with oxidative damage resulting from Mg-deficiency is unclear at the cellular level. To investigate whether hydrogen peroxide (H2O2) is associated in the oxidative stress induced by Mg-deficiency, the effect of Mg2+ deficiency (0, 0.4, 0.7 mM) on the metabolism of H2O2 was investigated in cultured chick embryo hepatocytes. After being cultured in the media with various concentrations of Mg2+ for 1, 2, 4, 6 and 10 days, parameters of H2O2 production, catalase activity, lipid peroxidation, intracellular total Mg and cell viability were analyzed. Results demonstrated that long-term incubation of chick embryo hepatocyte in extracellular Mg2+-deprivative and Mg2+-deficient (0.4 mM) states significantly enhanced the production of H2O2 (approximately twofold, respectively) and lipid peroxidation in the cell cultures, while decreasing the cell viability. Additionally, the reversing action of Mg2+ re-added to 1.0 mM and the partial reversing action of dimethylthiourea suggested that (i) [Mg2+]e deficiency induced the increase of H2O2 production, (ii) [Mg2+]e deficiency decreased catalase activity in chick embryo hepatocyte in vitro, subsequently causing oxidative stress and cell peroxidative damage.

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Authors and Affiliations

  1. Division of Animal Nutrition and Feed Science, College of Animal Science and Technology, China Agricultural University (CAU), Beijing, 100094, P.R. China

    Ying Yang, Jianmin Yuan, Wei Nie & Yuming Guo

  2. Division of Animal Physiology, College of Biological Sciences, CAU, Beijing, 100094, P.R. China

    Zhenlong Wu

  3. National Natural Science Foundation of China, Beijing, 100085, P.R. China

    Yue Chen

  4. Division of Basic Veterinary Medicine, College of Veterinary Medicine, CAU, Beijing, 100085, P.R. China

    Jian Qiao & Mingyu Gao

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  1. Ying Yang
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  2. Zhenlong Wu
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  3. Yue Chen
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  4. Jian Qiao
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  5. Mingyu Gao
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  6. Jianmin Yuan
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  7. Wei Nie
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  8. Yuming Guo
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Correspondence to Yuming Guo.

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Yang, Y., Wu, Z., Chen, Y. et al. Magnesium Deficiency Enhances Hydrogen Peroxide Production and Oxidative Damage in Chick Embryo Hepatocyte In Vitro. Biometals 19, 71–81 (2006). https://doi.org/10.1007/s10534-005-6898-1

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