Abstract
Type 2 diabetes mellitus affects up to 8% of the adult population in Western countries. Treatment of this disease with oral antidiabetic drugs is characterised by considerable interindividual variability in pharmacokinetics, clinical efficacy and adverse effects. Genetic factors are known to contribute to individual differences in bioavailability, drug transport, metabolism and drug action. Only scarce data exist on the clinical implications of this genetic variability on adverse drug effects or clinical outcomes in patients taking oral antidiabetics.
The polymorphic enzyme cytochrome P450 (CYP) 2C9 is the main enzyme catalysing the biotransformation of sulphonylureas. Total oral clearance of all studied sulphonylureas (tolbutamide, glibenclamide [glyburide], glimepiride, glipizide) was only about 20% in persons with the CYP2C9*3/*3 genotype compared with carriers of the wild-type genotype CYP2C9*1/*1, and clearance in the heterozygous carriers was between 50% and 80% of that of the wild-type genotypes. For reasons not completely known, the resulting differences in drug effects were much less pronounced. Nevertheless, CYP2C9 genotype-based dose adjustments may reduce the incidence of adverse effects. The magnitude of how doses might be adjusted can be derived from pharmacokinetic studies.
The meglitinide-class drug nateglinide is metabolised by CYP2C9. According to the pharmacokinetic data, moderate dose adjustments based on CYP2C9 genotypes may help in reducing interindividual variability in the antihyperglycaemic effects of nateglinide. Repaglinide is metabolised by CYP2C8 and, according to clinical studies, CYP2C8*3 carriers had higher clearance than carriers of the wild-type genotypes; however, this was not consistent with in vitro data and therefore further studies are needed. CYP2C8*3 is closely linked with CYP2C9*2.
CYP2C8 and CYP3A4 are the main enzymes catalysing biotransformation of the thiazolidinediones troglitazone and pioglitazone, whereas rosiglitazone is metabolised by CYP2C9 and CYP2C8. The biguanide metformin is not significantly metabolised but polymorphisms in the organic cation transporter (OCT) 1 and OCT2 may determine its pharmacokinetic variability.
In conclusion, pharmacogenetic variability plays an important role in the pharmacokinetics of oral antidiabetic drugs; however, to date, the impact of this variability on clinical outcomes in patients is mostly unknown and prospective studies on the medical benefit of CYP genotyping are required.
Similar content being viewed by others
References
Harris MI, Flegal KM, Cowie CC, et al. Prevalence of diabetes, impaired fasting glucose, and impaired glucose tolerance in US adults. The Third National Health and Nutrition Examination Survey, 1988–1994. Diabetes Care 1998; 21: 518–24
Harris MI. Diabetes in America: epidemiology and scope of the problem. Diabetes Care 1998; 21 Suppl. 3: C11–4
American Diabetes Association. Clinical practice recommendations 2001. Diabetes Care 2001; 24 Suppl. 1: S1–133
Clark Jr MJ, Sterrett JJ, Carson DS. Diabetes guidelines: a summary and comparison of the recommendations of the American Diabetes Association, Veterans Health Administration, and American Association of Clinical Endocrinologists. Clin Ther 2000; 22: 899–910; discussion 898
Ajdari A. Pumping liquids using asymmetric electrode arrays. Physical Review E 2000; 61: R45–8
Inzucchi SE. Oral antihyperglycemic therapy for type 2 diabetes: scientific review. Jama 2002; 287: 360–72
American Diabetes Association. Clinical practice guidelines. Alexandria (VA): American Diabetes Association, 2004
Carlsen SM. Sulfonylurea-induced hypoglycaemia: an iatrogenic and potentially fatal condition [in Norwegian]. Tidsskr Nor Laegeforen 1997; 117: 3079–82
Evans WE, McLeod HL. Pharmacogenomics: drug disposition, drug targets, and side effects. N Engl J Med 2003; 348: 538–49
Weinshilboum R. Inheritance and drug response. N Engl J Med 2003; 348: 529–37
Finta C, Zaphiropoulos PG. The human CYP2C locus: a prototype for intergenic and exon repetition splicing events. Genomics 2000; 63: 433–8
Miners JO, Birkett DJ. Cytochrome P4502C9: an enzyme of major importance in human drug metabolism. Br J Clin Pharmacol 1998; 45: 525–38
Ieiri I, Tainaka H, Morita T, et al. Catalytic activity of three variants (Ile, Leu, and Thr) at amino acid residue 359 in human CYP2C9 gene and simultaneous detection using single-strand conformation polymorphism analysis. Ther Drug Monit 2000; 22: 237–44
Sullivan-Klose TH, Ghanayem BI, Bell DA, et al. The role of the CYP2C9-Leu359 allelic variant in the tolbutamide polymorphism. Pharmacogenetics 1996; 6: 341–9
Stubbins MJ, Harries LW, Smith G, et al. Genetic analysis of the human cytochrome P450 CYP2C9 locus. Pharmacogenetics 1996; 6: 429–39
Yasar U, Eliasson E, Dahl ML, et al. Validation of methods for CYP2C9 genotyping: frequencies of mutant alleles in a Swedish population. Biochem Biophys Res Commun 1999; 254: 628–31
Williams PA, Cosme J, Ward A, et al. Crystal structure of human cytochrome P450 2C9 with bound warfarin. Nature 2003; 424: 464–8
Steward DJ, Haining RL, Henne KR, et al. Genetic association between sensitivity to warfarin and expression of CYP2C9*3. Pharmacogenetics 1997; 7: 361–7
Kidd RS, Straughn AB, Meyer MC, et al. Pharmacokinetics of chlorpheniramine, phenytoin, glipizide and nifedipine in an individual homozygous for the CYP2C9*3 allele. Pharmacogenetics 1999; 9: 71–80
Kirchheiner J, Bauer S, Meineke I, et al. Impact of CYP2C9 and CYP2C19 polymorphisms on tolbutamide kinetics and on the insulin and glucose response in healthy volunteers. Pharmacogenetics 2002; 12: 101–9
Bahadur N, Leathart JB, Mutch E, et al. CYP2C8 polymorphisms in Caucasians and their relationship with paclitaxel 6α-hydroxylase activity in human liver microsomes. Biochem Pharmacol 2002; 64: 1579–89
Yasar U, Lundgren S, Eliasson E, et al. Linkage between the CYP2C8 and CYP2C9 genetic polymorphisms. Biochem Biophys Res Commun 2002; 299: 25–8
Xie HG, Prasad HC, Kim RB, et al. CYP2C9 allelic variants: ethnic distribution and functional significance. Adv Drug Deliv Rev 2002; 54: 1257–70
Crespi CL, Miller VP. The R144C change in the CYP2C9*2 allele alters interaction of the cytochrome P450 with NADPH: cytochrome P450 oxidoreductase. Pharmacogenetics 1997; 7: 203–10
Furuya H, Fernandez Salguero P, Gregory W, et al. Genetic polymorphism of CYP2C9 and its effect on warfarin maintenance dose requirement in patients undergoing anticoagulation therapy. Pharmacogenetics 1995; 5: 389–92
Shon JH, Yoon YR, Kim KA, et al. Effects of CYP2C19 and CYP2C9 genetic polymorphisms on the disposition of and blood glucose lowering response to tolbutamide in humans. Pharmacogenetics 2002; 12: 111–9
Bidstrup TB, Bjornsdottir I, Sidelmann UG, et al. CYP2C8 and CYP3A4 are the principal enzymes involved in the human in vitro biotransformation of the insulin secretagogue repaglinide. Br J Clin Pharmacol 2003; 56: 305–14
Wang JS, Neuvonen M, Wen X, et al. Gemfibrozil inhibits CYP2C8-mediated cerivastatin metabolism in human liver microsomes. Drug Metab Dispos 2002; 30: 1352–6
Soyama A, Hanioka N, Saito Y, et al. Amiodarone N-deethylation by CYP2C8 and its variants, CYP2C8*3 and CYP2C8 P404A. Pharmacol Toxicol 2002; 91: 174–8
Dai D, Zeldin DC, Blaisdell JA, et al. Polymorphisms in human CYP2C8 decrease metabolism of the anticancer drug paclitaxel and arachidonic acid. Pharmacogenetics 2001; 11: 597–607
Lee CR, Pieper JA, Hinderliter AL, et al. Evaluation of cytochrome P4502C9 metabolic activity with tolbutamide in CYP2C91 heterozygotes. Clin Pharmacol Ther 2002; 72: 562–71
Ferner RE, Chaplin S. The relationship between the pharmacokinetics and pharmacodynamic effects of oral hy-poglycaemic drugs. Clin Pharmacokinet 1987; 12: 379–401
Tolbutamide (Rastinon®) prescribing information. Frankfurt: Hoechst AG, 2001
Niemi M, Cascorbi I, Timm R, et al. Glyburide and glimepiride pharmacokinetics in subjects with different CYP2C9 genotypes. Clin Pharmacol Ther 2002; 72: 326–32
Badian MKA, Lehr KH, Malercyk V, et al. Pharmakokinetik und Pharmakodynamik nach intravenöser Verabreichung des Hydroxymetaboliten (M1) von Glimepirid (HQE 490). Naunyn Schmiedebergs Arch Pharmacol 1993; 347 Suppl.: R27
Glimepride (Amaryl®) prescribing information. Bridgewater (MA): Aventis Pharmaceuticals Inc., 2001
Glibenclamide (Euglucon®) prescribing information. Frankfurt: Hoechst AG, 2001
Gliclazide (Diamicron®) prescribing information. Munich: Servier GmbH, 2001
Weaver ML, Orwig BA, Rodriguez LC, et al. Pharmacokinetics and metabolism of nateglinide in humans. Drug Metab Dispos 2001; 29: 415–21
McLeod J. Clinical pharmacokinetics of nateglinide. Clin Pharmacokinet 2004; 43: 97–120
Hatorp V. Clinical pharmacokinetics and pharmacodynamics of repaglinide. Clin Pharmacokinet 2002; 41: 471–83
Budde K, Neumayer HH, Fritsche L, et al. The pharmacokinetics of pioglitazone in patients with impaired renal function. Br J Clin Pharmacol 2003; 55: 368–74
Pioglitazone (Actos®) prescribing information. Osaka: Takeda Pharma, 2001
Chapelsky MC, Thompson-Culkin K, Miller AK, et al. Pharmacokinetics of rosiglitazone in patients with varying degrees of renal insufficiency. J Clin Pharmacol 2003; 43: 252–9
Rosiglitazone (Avandia®) prescribing information. Research Triangle Park (NC): GlaxoSmithKline, 2001
Metformin (Glucophage®) prescribing information. Princeton (NJ): Bristol-Myers Squibb Company, 2004
De Fronzo RA. Pharmacologic therapy for type 2 diabetes mellitus. Ann Intern Med 1999; 131: 281–303
Thomas RC, Ikeda GJ. The metabolic fate of tolbutamide in man and in the rat. J Med Chem 1966; 9: 507–10
Miners JO, Birkett DJ. Use of tolbutamide as a substrate probe for human hepatic cytochrome P450 2C9. Methods Enzymol 1996; 272: 139–45
Veronese ME, Miners JO, Randies D, et al. Validation of the tolbutamide metabolic ratio for population screening with use of sulfaphenazole to produce model phenotypic poor metabolizers. Clin Pharmacol Ther 1990; 47: 403–11
Scott J, Poffenbarger PL. Pharmacogenetics of tolbutamide metabolism in humans. Diabetes 1979; 28: 41–51
Page MA, Boutagy JS, Shenfield GM. A screening test for slow metabolisers of tolbutamide. Br J Clin Pharmacol 1991; 31: 649–54
Peart GF, Boutagy J, Shenfield GM. Lack of relationship between tolbutamide metabolism and debrisoquine oxidation phenotype. Eur J Clin Pharmacol 1987; 33: 397–402
Miners JO, Wing LM, Birkett DJ. Normal metabolism of debrisoquine and theophylline in a slow tolbutamide metaboliser. Aust N Z J Med 1985; 15: 348–9
Veronese ME, Miners JO, Rees DL, et al. Tolbutamide hydroxylation in humans: lack of bimodality in 106 healthy subjects. Pharmacogenetics 1993; 3: 86–93
Jackson JE, Bressier R. Clinical pharmacology of sulphonylurea hypoglycaemic agents: part 1. Drugs 1981; 22: 211–45
Relling MV, Aoyama T, Gonzalez FJ, et al. Tolbutamide and mephenytoin hydroxylation by human cytochrome P450s in the CYP2C subfamily. J Pharmacol Exp Ther 1990; 252: 442–7
Veronese ME, Mackenzie PI, Doecke CJ, et al. Tolbutamide and Phenytoin hydroxylations by cDNA-expressed human liver cytochrome P4502C9 [published erratum appears in Biochem Biophys Res Commun 1991 Nov 14; 180 (3); 1527]. Biochem Biophys Res Commun 1991; 175: 1112–8
Srivastava PK, Yun CH, Beaune PH, et al. Separation of human liver microsomal tolbutamide hydroxylase and (S)-mephenytoin 4′-hydroxylase cytochrome P-450 enzymes. Mol Pharmacol 1991; 40: 69–79
Wester MR, Lasker JM, Johnson EF, et al. CYP2C19 participates in tolbutamide hydroxylation by human liver microsomes. Drug Metab Dispos 2000; 28: 354–9
Knodell RG, Hall SD, Wilkinson GR, et al. Hepatic metabolism of tolbutamide: characterization of the form of cytochrome P-450 involved in methyl hydroxylation and relationship to in vivo disposition. J Pharmacol Exp Ther 1987; 241: 1112–9
Jetter A, Kinzig-Schippers M, Skott A, et al. Cytochrome P(450) 2C9 phenotyping using low-dose tolbutamide. Eur J Clin Pharmacol 2004; 60: 165–71
Yuan R, Madani S, Wei XX, et al. Evaluation of cytochrome P450 probe substrates commonly used by the pharmaceutical industry to study in vitro drug interactions. Drug Metab Dispos 2002; 30: 1311–9
Rupp W, Christ O, Fulberth W. Studies on the bioavailability of glibenclamide [in German]. Arzneimittelforschung 1972; 22: 471–3
Rydberg T, Jonsson A, Roder M, et al. Hypoglycemic activity of glyburide (glibenclamide) metabolites in humans. Diabetes Care 1994; 17: 1026–30
Jonsson A, Hallengren B, Rydberg T, et al. Effects and serum levels of glibenclamide and its active metabolites in patients with type 2 diabetes. Diabetes Obes Metab 2001; 3: 403–9
Jonsson A, Rydberg T, Ekberg G, et al. Slow elimination of glyburide in NIDDM subjects. Diabetes Care 1994; 17: 142–5
Melander A, Lebovitz HE, Faber OK. Sulfonylureas: why, which, and how?. Diabetes Care 1990; 13 Suppl. 3: 18–25
Dahl-Puustinen ML, Alm C, Bertilsson L, et al. Lack of relationship between glibenclamide metabolism and debrisoquine or mephenytoin hydroxylation phenotypes. Br J Clin Pharmacol 1990; 30: 476–80
Kirchheiner J, Brockmöller J, Meineke I, et al. Impact of CYP2C9 amino acid polymorphisms on glyburide kinetics and on the insulin and glucose response in healthy volunteers. Clin Pharmacol Ther 2002; 71: 286–96
Holstein AP, Ptak A, Egberts M, et al. Association between CYP2C9 slow metabolizer genotypes and severe hypoglycemia in medication with sulfonylurea antidiabetics. Br J Clin Pharmacol 2005; 60: 103–6
Asplund Wiholm BE, Lithner F. Glibenclamide-associated hypoglycaemia: a report on 57 cases. Diabetologia 1983; 24: 412–7
Axelgaard G, Skensved H, Asfeldt VH. Hypoglycemia during treatment with sulfonylurea preparations [in Danish]. Ugeskr Laeger 1986; 148: 2155–8
Berger W, Caduff F, Pasquel M, et al. The relatively frequent incidence of severe sulfonylurea-induced hypoglycemia in the last 25 years in Switzerland: results of 2 surveys in Switzerland in 1969 and 1984 [in German]. Schweiz Med Wochenschr 1986; 116: 145–51
Holstein A, Egberts EH. Risk of hypoglycaemia with oral antidiabetic agents in patients with type 2 diabetes. Exp Clin Endocrinol Diabetes 2003; 111: 405–14
Wahlin-Boll E, Aimer LO, Melander A. Bioavailability, pharmacokinetics and effects of glipizide in type 2 diabetics. Clin Pharmacokinet 1982; 7: 363–72
Chung M, Kourides I, Canovatchel W, et al. Pharmacokinetics and pharmacodynamics of extended-release glipizide GITS compared with immediate-release glipizide in patients with type II diabetes mellitus. J Clin Pharmacol 2002; 42: 651–7
Fuccella LM, Tamassia V, Valzelli G. Metabolism and kinetics of the hypoglycemic agent glipizide in man: comparison with glibenclamide. J Clin Pharmacol New Drugs 1973; 13: 68–75
Kidd RS, Curry TB, Gallagher S, et al. Identification of a null allele of CYP2C9 in an African-American exhibiting toxicity to Phenytoin. Pharmacogenetics 2001; 11: 803–8
Rosenkranz Pharmacokinetic basis for the safety of glimepiride in risk groups of NIDDM patients. Horm Metab Res 1996; 28: 434-9
Wang R, Chen K, Wen S-Y, et al. Pharmacokinetics of glimepiride and cytochrome P450 2C9 genetic polymorphisms. Clin Pharmacol Ther 2005 Jul; 78_(1): 90–2
Palmer KJ, Brogden RN. Gliclazide: an update of its pharmacological properties and therapeutic efficacy in non-insulin-dependent diabetes mellitus. Drugs 1993; 46: 92–125
Rieutord A, Stupans I, Shenfield GM, et al. Gliclazide hydroxyl-ation by rat liver microsomes. Xenobiotica 1995; 25: 1345–54
Huupponen R, Viikari J, Saarimaa H. Chlorpropamide and glibenclamide serum concentrations in hospitalized patients. Ann Clin Res 1982; 14: 119–22
Neuvonen PJ, Karkkainen S, Lehtovaara R. Pharmacokinetics of chlorpropamide in epileptic patients: effects of enzyme induction and urine pH on chlorpropamide elimination. Eur J Clin Pharmacol 1987; 32: 297–301
Yoon YR, Shon JH, Kim KA, et al. Pharmacokinetics and pharmacodynamics of tolbutamide and chlorpropamide in relation to CYP2C9 and CYP2C19 [poster]. Clin Pharmacol Ther 2000; 67: 152
Galloway JA, McMahon RE, Culp HW, et al. Metabolism, blood levels and rate of excretion of acetohexamide in human subjects. Diabetes 1967; 16: 118–27
Fujitani S, Yada T. A novel D-phenylalanine-derivative hypoglycemic agent A-4166 increases cytosolic free Ca2+ in rat pancreatic beta-cells by stimulating Ca2+ influx. Endocrinology 1994; 134: 1395–400
Akiyoshi M, Kakei M, Nakazaki M, et al. A new hypoglycemic agent, A-4166, inhibits ATP-sensitive potassium channels in rat pancreatic beta-cells. Am J Physiol 1995; 268: E185–93
Marre M, Van Gaal L, Usadel KH, et al. Nateglinide improves glycaemic control when added to metformin monotherapy: results of a randomized trial with type 2 diabetes patients. Diabetes Obes Metab 2002; 4: 177–86
Kalbag JB, Walter YH, Nedelman JR, et al. Mealtime glucose regulation with nateglinide in healthy volunteers: comparison with repaglinide and placebo. Diabetes Care 2001; 24: 73–7
Takesada H, Matsuda K, Ohtake R, et al. Structure determination of metabolites isolated from urine and bile after administration of AY4166, a novel D-phenylalanine-derivative hypoglycemic agent. Bioorg Med Chem 1996; 4: 1771–81
Cao G, Song Y. Pharmacokinetics of enantiomers of a new antidiabetic agent (AY4166) in healthy subjects and its metabolism using isolated rats hepatocytes. Clin Pharmacol Ther 2002; 71: P 100
Hatorp V, Walther KH, Christensen MS, et al. Single-dose pharmacokinetics of repaglinide in subjects with chronic liver disease. J Clin Pharmacol 2000; 40: 142–52
Kirchheiner J, Meineke I, Muller G, et al. Influence of CYP2C9 and CYP2D6 polymorphisms on the pharmacokinetics of nateglinide in genotyped healthy volunteers. Clin Pharmacokinet 2004; 43(4): 267–78
Niemi M, Leathart JB, Neuvonen M, et al. Polymorphism in CYP2C8 is associated with reduced plasma concentrations of repaglinide. Clin Pharmacol Ther 2003; 74: 380–7
Niemi M, Neuvonen PJ, Kivisto KT. The cytochrome P4503A4 inhibitor clarithromycin increases the plasma concentrations and effects of repaglinide. Clin Pharmacol Ther 2001; 70: 58–65
Niemi M, Backman JT, Neuvonen M, et al. Effects of rifampin on the pharmacokinetics and pharmacodynamics of glyburide and glipizide. Clin Pharmacol Ther 2001; 69: 400–6
Yamazaki H, Shibata A, Suzuki M, et al. Oxidation of troglitazone to a quinone-type metabolite catalyzed by cytochrome P-450 2C8 and P-450 3A4 in human liver microsomes. Drug Metab Dispos 1999; 27: 1260–6
Hewitt NJ, Lloyd S, Hayden M, et al. Correlation between troglitazone cytotoxicity and drug metabolic enzyme activities in cryopreserved human hepatocytes. Chem Biol Interact 2002; 142: 73–82
Kassahun K, Pearson PG, Tang W, et al. Studies on the metabolism of troglitazone to reactive intermediates in vitro and in vivo: evidence for novel biotransformation pathways involving quinone methide formation and thiazolidinedione ring scission. Chem Res Toxicol 2001; 14: 62–70
Kumashiro R, Kubota T, Koga Y, et al. Association of troglitazone-induced liver injury with mutation of the cytochrome P450 2C19 gene. Hepatol Res 2003; 26: 337–42
Baldwin SJ, Clarke SE, Chenery RJ. Characterization of the cytochrome P450 enzymes involved in the in vitro metabolism of rosiglitazone. Br J Clin Pharmacol 1999; 48: 424–32
Nowak SN, Edwards DJ, Clarke A, et al. Pioglitazone. J Clin Pharmacol 2002; 42: 1299–302
Hanefeld M. Pharmacokinetics and clinical efficacy of pioglitazone. Int J Clin Pract Suppl 2001; (121): 19–25
Scheen AJ, Hanefeld M. Hepatotoxicity with thiazolidinediones: is it a class effect?. Drug Saf 2001; 24: 873–88
Wahlin-Boll E, Sartor G, Melander A, et al. Impaired effect of sulfonylurea following increased dosage. Eur J Clin Pharmacol 1982; 22: 21–5
American Association of Diabetes Educators. Intensive diabetes management: implications of the DCCT and UKPDS. Diabetes Educ 2002; 28: 735–40
Brockmöller J, Kirchheiner J, Meisel C, et al. Pharmacogenetic diagnostics of cytochrome P450 polymorphisms in clinical drug development and in drug treatment. Pharmacogenomics 2000; 1: 125–51
Kirchheiner J, Brøsen K, Dahl ML, et al. CYP2D6 and CYP2C19 genotype-based dose recommendations for antidepressants: a first step towards subpopulation-specific dosages. Acta Psychiatr Scand 2001; 104: 173–92
Mudaliar S, Chang AR, Henry RR. Thiazolidinediones, peripheral edema, and type 2 diabetes: incidence, pathophysiology, and clinical implications. Endocr Pract 2003; 9: 406–16
Takeda M, Khamdang S, Narikawa S, et al. Human organic anion transporters and human organic cation transporters mediate renal antiviral transport. J Pharmacol Exp Ther 2002; 300: 918–24
Kerb R, Brinkmann U, Chatskaia N, et al. Identification of genetic variations of the human organic cation transporter hOCT1 and their functional consequences. Pharmacogenetics 2002; 12: 591–5
Acknowledgements
Financial support for this work was given by the German Ministry of Education and Research (GG 9845/5 and 03/4507) and by the German Research Foundation (KI 842/5-1). The authors have no conflicts of interest that are directly relevant to the content of this review.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kirchheiner, J., Roots, I., Goldammer, M. et al. Effect of Genetic Polymorphisms in Cytochrome P450 (CYP) 2C9 and CYP2C8 on the Pharmacokinetics of Oral Antidiabetic Drugs. Clin Pharmacokinet 44, 1209–1225 (2005). https://doi.org/10.2165/00003088-200544120-00002
Published:
Issue Date:
DOI: https://doi.org/10.2165/00003088-200544120-00002