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Effect of oxygen on spin-spin and spin-lattice relaxation in DOPA-melanin. Complexes with chloroquine and metal ions

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Abstract

Electron paramagnetic resonance (EPR) studies of complexes of chloroquine with melanin synthesized from 3,4-dihydroxyphenylalanine (DOPA) confirmed an important role ofo-semiquinone free radicals during the drug binding by melanin. Chloroquine increases free radical concentration in DOPA-melanin. It has been also demonstrated that chloroquine binding to melanin is modified by metal ions. Co2+ ions reduce and Zn2+ ions increase the concentration of free radicals in both DOPA-melanin and melanin-chloroquine complexes. Free radical properties of melanin strongly depend on the presence of oxygen (O2) in the environment of the sample. Interactions of melanin and oxygen molecules cause a decrease of the free radical concentration in the samples and a narrowing of the EPR lines. Higher values of both spin-spin and spin-lattice relaxation times were determined for melanin samples in air than for evacuated samples. Application of the observed effects in oximetry is suggested.

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References

  1. Buszman E.: Habilitation thesis, Medical University of Silesia, Katowice, Poland 1994.

  2. Buszman E., Kopera M., Wilczok T.: Biochem. Pharmacol.33, 7–11 (1984)

    Article  Google Scholar 

  3. Pasenkiewicz-Gierula M., Sealy R.C.: Biochim. Biophys. Acta884, 510–516 (1986)

    Google Scholar 

  4. Sarna T., Hyde J.S., Swartz H.M.: Science192, 1132–2234 (1976)

    Article  ADS  Google Scholar 

  5. Sealy R.C., Felix C.C., Hyde J.S., Swartz H.M. in: Free Radicals in Biology (Pryor W.A., ed.), vol. IV, pp. 209–259. New York: Academic Press 1980.

    Google Scholar 

  6. Jastrzębska M.M., Isotalo H., Paloheimo J., Stubb H., Pilawa B.: J. Biomater. Sci. Polym. Ed.7, 781–793 (1996)

    Article  Google Scholar 

  7. Pilawa B., Buszman E., Latocha M., Wilczok T. in: Proceedings of the International Conference on Medical Physics and Engineering in Health Care jointly held with 12th Congress of the Polish Society of Medical Physics, p. 49. Poznań 2001.

  8. Binns F., Chapman R.F., Robson N.C., Swan G.A., Waggott A.J.: J. Chem. Soc. C1970, 1128–1134.

  9. Wertz J.E., Bolton J.R.: Electron Spin Resonance: Elementary Theory and Practical Applications. New York: Chapman and Hall 1986.

    Google Scholar 

  10. Sczaniecki B. in: Radiospektroskopia Ciała Stałego (Stankowski J., ed.), pp. 233–251. Warszawa: Państwowe Wydawnictwo Naukowe 1975.

    Google Scholar 

  11. Stankowski J., Kuczyński W.: Fiz. Dielektr. Radiospektr.4, 249–255 (1966)

    Google Scholar 

  12. Pilawa B., Więckowski A.B.: Fuel76, 1173–1177 (1997)

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Molecular Biology, Biochemistry and Biopharmacy, School of Pharmacy, Medical University of Silesia, Narcyzów 1, 41-200, Sosnowiec, Poland

    B. Pilawa, M. Latocha & T. Wilczok

  2. Department Pharmaceutical Chemistry, School of Pharmacy, Medical University of Silesia, Sosnowiec, Poland

    E. Buszman

  3. Department of Medical Physics, School of Pharmacy, Medical University of Silesia, Narcyzów 1, 41-200, Sosnowiec, Poland

    B. Pilawa

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  1. B. Pilawa
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  2. M. Latocha
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  3. E. Buszman
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  4. T. Wilczok
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Correspondence to B. Pilawa.

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Pilawa, B., Latocha, M., Buszman, E. et al. Effect of oxygen on spin-spin and spin-lattice relaxation in DOPA-melanin. Complexes with chloroquine and metal ions. Appl. Magn. Reson. 25, 105–111 (2003). https://doi.org/10.1007/BF03166970

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  • DOI: https://doi.org/10.1007/BF03166970

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