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Electrospray ionization and matrix assisted laser desorption/ionization mass spectrometry: Powerful analytical tools in recombinant protein chemistry

Nature Biotechnology volume 14, pages 449–457 (1996)Cite this article

Abstract

Electrospray ionization and matrix assisted laser desorption/ionization are effective ionization methods for mass spectrometry of biomolecules. Here we describe the capabilities of these methods for peptide and protein characterization in biotechnology. An integrated analytical strategy is presented encompassing protein characterization prior to and after cloning of the corresponding gene.nature.

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  1. Peter Roepstorff: Corresponding author (e-mail: roe@pr–group.ou.dk).

Authors and Affiliations

  1. Department of Molecular Biology, Odense University, Campusvej 55, DK-5230, Odense M, Denmark

    Jens S. Andersen

  2. Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Copenhagen Valby, Denmark

    Birte Svensson

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Andersen, J., Svensson, B. & Roepstorff, P. Electrospray ionization and matrix assisted laser desorption/ionization mass spectrometry: Powerful analytical tools in recombinant protein chemistry. Nat Biotechnol 14, 449–457 (1996). https://doi.org/10.1038/nbt0496-449

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