Abstract
Some disadvantages of commercial dressings have mobilized research groups to clinically improve these materials considering their cost–benefit ratio. Tissue engineering has emerged as a field that uses biomaterials in the form of scaffolds mimicking human morphofunctionality and their combination with cells in order to repair or replace damaged tissues. There is still a need for customization and characterization of the material to ensure its safety. Therefore, this study aimed to perform further characterizations of the gelatin (Gel)–coated poly(ε-caprolactone) (PCL) matrix produced in previous studies by rotary jet spinning. PCL fibers produced by rotary jet spinning were submersed in a Gel solution, inspired by the dip-coating method, followed by crosslinking with glutaraldehyde. The material was then submitted to physicochemical and biological characterization. The thermal properties were confirmed by differential scanning calorimetry. Significant differences were observed in the fiber thickness and swelling of PCL/Gel compared to PCL, while fiber spacing was greater for PCL. The crosslinked Gel appeared to increase the tensile strength of PCL, and the thermal stability of PCL/Gel was higher than that of Gel alone. In addition to the absence of cytotoxicity of the materials, the cytochemical data suggest higher cell activity on the PCL/Gel scaffold. Scanning electron microscopy reinforces this observation, indicating promising applications of PCL/Gel scaffolds in skin tissue engineering.
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The authors would like to thank the Multiuser Experimental Centers (CEM) of the Federal University of ABC (UFABC), Santo André Campus and São Bernardo do Campo Campus.
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Giorno, L.P., Rodrigues, L.R. & Santos, A.R. Characterization and in vitro analysis of a poly(ε-caprolactone)–gelatin matrix produced by rotary jet spinning and applied as a skin dressing. Polym. Bull. 79, 9131–9158 (2022). https://doi.org/10.1007/s00289-022-04228-9
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DOI: https://doi.org/10.1007/s00289-022-04228-9