Abstract
Colon cancer is the third most common cancer in Brazil and in the world. Despite the high incidence and high mortality rate, most of the cases could be prevented by using accurate techniques for early cancer detection. In this sense, it is important to conduct preclinical studies using animal models of colon diseases, which requires the implementation of appropriate instrumentation. Therefore, a technique to generate three dimensional endoluminal ultrasound biomicroscopic images (eUBM-3D) of the mouse colon was developed using an ultrasound system operating at 40 MHz. The eUBM-2D (bi-dimensional) images were acquired and used to construct the eUBM-3D images. The technique was initially tested in a phantom mimicking the colon of small animals and subsequently in control- or tumor-bearing mouse colon, in vivo, with some of the eUBM-2D images segmented to highlight the region of interest. The eUBM-3D system allowed the identification of colon layers and lesions, or tumor, in both phantom and mouse colon.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Nacional Cancer Institute: Homepage. http://seer.cancer.gov/statfacts/html/colorect.html. Accessed 16 Apr 2018
Instituto Nacional de Câncer: Homepage. http://www.inca.gov.br/estimativa/2018/sintese-de-resultados-comentarios.asp. Accessed 16 Apr 2018
American Cancer Society: Colorectal cancer facts & figures 2011–2013. American Cancer Society, Atlanta, p. 4 (2011)
Soletti, R.C., Alves de, K.Z., Britto de M.A.P., Matos de D.G., Soldan, M., Borges, H.L., Machado, J.C.: Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy. World J. Gastroenterol. https://doi.org/10.3748/wjg.v19.i44.8056 (2013)
Ryan, L.K., Foster, F.S.: Tissue equivalent vessel phantom for intravascular ultrasound. Ultrasound Med. Biol. 23(2), 261–273 (1997)
Oliveira, R.P., Geremia, J., Soletti, R.C., Machado, J.C.: Geração de imagens 3D de Biomicroscopia Ultrassônica endoluminal do cólon de camundongo XXV Congresso Brasileiro de Engenharia Biomédica (2016)
Chorilli, M., Michelin, D.C., Salgado, H.R.N.: Animais de Laboratório: o camundongo. Revista de Ciências Farmacêuticas Básica e Aplicada 28(1), 11–23 (2007)
Alves, K.Z., Soletti, R.C., Britto de, M.A.P., Matos de, D.G., Soldan, M., Borges, H.L., Machado, J.C.: In vivo endoluminal ultrasound biomicroscopic imaging in a mouse modelo of colorectal cancer. Acad. Radiol. 20(1) (2013)
Soletti, R.C., Britto de, M.A.P., Borges, H.L., Machado, J.C.: Analysis of tumor morphology and vasculature in an animal model of colorectal cancer using in vivo contrast-enhanced endoluminal ultrasound biomicroscopy. In: Ultrasonics Symposium (IUS), 2012 IEEE International (2012)
Tanaka, T.: Colorectal carcinogenesis: review of human and experimental animal studies. J. Carcinog 8, 1–19 (2009)
Acknowledgements
We gratefully acknowledge financial support from CAPES, CNPq and FAPERJ.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Ethics declarations
The authors certify that they have no conflict of interests in the subject matter or materials discussed in this manuscript.
Rights and permissions
Copyright information
© 2019 Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
de Oliveira, R.P., Solleti, R.C., Machado, J.C. (2019). 3D-Endoluminal Ultrasound Biomicroscopy of Mouse Colon Tumor and Mimicking Phantom. In: Costa-Felix, R., Machado, J., Alvarenga, A. (eds) XXVI Brazilian Congress on Biomedical Engineering. IFMBE Proceedings, vol 70/1. Springer, Singapore. https://doi.org/10.1007/978-981-13-2119-1_62
Download citation
DOI: https://doi.org/10.1007/978-981-13-2119-1_62
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-13-2118-4
Online ISBN: 978-981-13-2119-1
eBook Packages: EngineeringEngineering (R0)