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Studies on transformation of Escherichia coli with plasmids | Journal of Molecular Biology | 1983 | 9.5K |
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14 | Cell | 1993 | 9.2K |
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity | Science | 2012 | 8.8K |
Quantitative monitoring of gene expression patterns with a complementary DNA microarray | Science | 1995 | 7.6K |
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter | Nucleic Acids Research | 1984 | 6.5K |
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers | Gene | 1982 | 6.1K |
Genome engineering using the CRISPR-Cas9 system | Nature Protocols | 2013 | 6.1K |
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase | Gene | 1988 | 5.7K |
Use of T7 RNA polymerase to direct expression of cloned genes | Methods in Enzymology | 1990 | 5.6K |
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes | Journal of Molecular Biology | 1986 | 5.5K |
Revised methods for the Salmonella mutagenicity test | Mutation Research - Environmental Mutagenesis and Related Subjects Including Methodology | 1983 | 5.4K |
A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine | Proceedings of the National Academy of Sciences of the United States of America | 1995 | 5.3K |
Construction and characterization of new cloning vehicle. II. A multipurpose cloning system | Gene | 1977 | 5.1K |
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases | Cell | 1993 | 5.1K |
Screening lambdagt recombinant clones by hybridization to single plaques in situ | Science | 1977 | 4.7K |
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes | Cell | 1986 | 4.4K |
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters | Proceedings of the National Academy of Sciences of the United States of America | 1992 | 4.3K |
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae | Yeast | 1998 | 4.1K |
In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector | Science | 1996 | 4.1K |
A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae | Nature | 2000 | 4K |
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter | Journal of Bacteriology | 1995 | 3.9K |
Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus | Nature | 2003 | 3.9K |
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid | Journal of Bacteriology | 1978 | 3.8K |
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans | Nature | 2000 | 3.7K |