Most Cited Articles of Plasmid

Sequencing end-labeled DNA with base-specific chemical cleavagesMethods in Enzymology198012.8K
Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology19839.5K
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14Cell19939.2K
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunityScience20128.8K
Quantitative monitoring of gene expression patterns with a complementary DNA microarrayScience19957.6K
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterNucleic Acids Research19846.5K
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene19826.1K
Genome engineering using the CRISPR-Cas9 systemNature Protocols20136.1K
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene19885.7K
Use of T7 RNA polymerase to direct expression of cloned genesMethods in Enzymology19905.6K
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesJournal of Molecular Biology19865.5K
Revised methods for the Salmonella mutagenicity testMutation Research - Environmental Mutagenesis and Related Subjects Including Methodology19835.4K
A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimineProceedings of the National Academy of Sciences of the United States of America19955.3K
Construction and characterization of new cloning vehicle. II. A multipurpose cloning systemGene19775.1K
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinasesCell19935.1K
Screening lambdagt recombinant clones by hybridization to single plaques in situScience19774.7K
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomesCell19864.4K
Tight control of gene expression in mammalian cells by tetracycline-responsive promotersProceedings of the National Academy of Sciences of the United States of America19924.3K
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiaeYeast19984.1K
In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vectorScience19964.1K
A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiaeNature20004K
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoterJournal of Bacteriology19953.9K
Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirusNature20033.9K
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmidJournal of Bacteriology19783.8K
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegansNature20003.7K