N-terminal labeling of proteins by the Pictet–Spengler reaction
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This work describes the methodology for the N-terminal modification of proteins by the Pictet–Spengler reaction.
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Acknowledgments
We are grateful to Prof. Hiroshi Nishihara and Dr. Shoko Kume of the University of Tokyo, for help in measuring MALDI mass spectra and to Masaru Kinugawa of the University of Tokyo for help in measuring NMR spectra. Thanks are also due to Prof. Shigeyuki Yokoyama, Dr. Takuhiro Ito and Shinya Mimasu of the University of Tokyo, for help in HPLC purification and UV/vis absorption measurement and to Dr. Hiroshi Nakayama of RIKEN for valuable suggestion. This work was partly supported by a
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