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The effects of the phosphodiesterase type V inhibitor sildenafil on human and bovine retinal function in vitro

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Abstract

Background

After the ingestion of sildenafil (Viagra), visual adverse events have been reported, possibly caused by an inhibition of the phototransduction cascade by sildenafil via phosphodiesterase (PDE 6). Therefore, we investigated the effects of sildenafil on photoreceptors and postsynaptic neurons of human and bovine retinas using the isolated superfused vertebrate retina technique.

Methods

Human and bovine retina preparations were perfused with an oxygen preequilibrated standard solution. The electroretinogram (ERG) was recorded using Ag/AgCl electrodes. After recording stable ERG amplitudes, sildenafil was added to the solution for 45 min. Thereupon, the preparations were reperfused with standard solution for 240 min.

Results

Following the application of sildenafil (3 μMol/l), the b-wave amplitude of bovine and human preparations was reduced continuously and disappeared completely. After reperfusion with the standard solution for 4 h, the b-wave amplitude did not recover completely. Using the same sildenafil concentration (3 μMol/l), the a-wave amplitude of the human retina was not totally abolished, but reduced to 21% of the initial amplitude and remained reduced at washout. For all retinal preparations, the implicit time of the ERG amplitudes remained significantly extended at the end of the washout.

Conclusions

Strong similarities were detected in the drug-induced changes of the ERG when comparing human and bovine retinas. The results suggest that sildenafil impairs retinal function at not only the level of the photoreceptors, but it also affects the neuronal network of the inner retina at concentrations of approximately 30-fold higher than at therapeutic plasma concentrations.

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Acknowledgments

We thank Dr. Michael Partsch for proofreading this manuscript.

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Authors and Affiliations

  1. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany

    Matthias Lüke, Toni Schneider & Christoph Lüke

  2. Center of Molecular Medicine Cologne (CMMC), University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany

    Toni Schneider

  3. University Eye Hospital, Centre for Ophthalmology, Eberhard-Karls University of Tuebingen, Schleichstr. 12-16, 72076, Tuebingen, Germany

    Matthias Lüke & Peter Szurman

  4. Center of Ophthalmology, University of Cologne, Joseph-Stelzmann-Str. 9, 50924, Köln, Germany

    Christoph Lüke

  5. Department of Ophthalmology I, University of Tuebingen, Schleichstr. 12-16, 72076, Tuebingen, Germany

    Matthias Lüke

Authors
  1. Matthias Lüke
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  2. Peter Szurman
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  3. Toni Schneider
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  4. Christoph Lüke
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Corresponding author

Correspondence to Matthias Lüke.

Additional information

Dedicated to the late Prof. Dr. Werner Sickel, who passed away on 18th December 2004.

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Lüke, M., Szurman, P., Schneider, T. et al. The effects of the phosphodiesterase type V inhibitor sildenafil on human and bovine retinal function in vitro. Graefes Arch Clin Exp Ophthalmol 245, 1211–1215 (2007). https://doi.org/10.1007/s00417-007-0562-0

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  • DOI: https://doi.org/10.1007/s00417-007-0562-0

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