18.8(top 1%)
Impact Factor
20.1(top 1%)
extended IF
289(top 1%)
H-Index
5.3K
authors
3.2K
papers
454.2K
citations
9.3K
citing journals
331.9K
citing authors

Most Cited Articles of Nature Protocols

TitleYearCitations
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources200924.4K
Analyzing real-time PCR data by the comparative C(T) method200815.9K
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks20128.3K
Genome engineering using the CRISPR-Cas9 system20136.2K
The Phyre2 web portal for protein modeling, prediction and analysis20155.6K
Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm20095.1K
De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis20134.6K
I-TASSER: a unified platform for automated protein structure and function prediction20104.4K
Protein structure prediction on the Web: a case study using the Phyre server20093.6K
In-gel digestion for mass spectrometric characterization of proteins and proteomes20063.4K
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis20072.9K
Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances20082.9K
In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro20072.9K
Locating proteins in the cell using TargetP, SignalP and related tools20072.6K
Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips20072.5K
Morris water maze: procedures for assessing spatial and related forms of learning and memory20062.5K
Clonogenic assay of cells in vitro20062.5K
Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown20162.3K
Using circular dichroism spectra to estimate protein secondary structure20062.2K
Sulforhodamine B colorimetric assay for cytotoxicity screening20062.2K
Full-length RNA-seq from single cells using Smart-seq220142K
Tricine-SDS-PAGE20061.8K
Integration of biological networks and gene expression data using Cytoscape20071.8K
Materials fabrication from Bombyx mori silk fibroin20111.8K
High-resolution in situ hybridization to whole-mount zebrafish embryos20081.7K