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Arabinoxylan consumption decreases postprandial serum glucose, serum insulin and plasma total ghrelin response in subjects with impaired glucose tolerance

Abstract

Objective:

Arabinoxylan (AX) consumption is associated with metabolic improvement during diabetes and with modulation of ghrelin, an orexigenic gut hormone. The effect of AX consumption on ghrelin secretion in disturbed metabolic states is unknown. Therefore, we investigated the postprandial responses to AX consumption of serum glucose, insulin and triglycerides and plasma total and acylated ghrelin in subjects with impaired glucose tolerance (IGT).

Design:

Randomized, single-blind, controlled, crossover intervention trial.

Subjects:

Seven female and four male adults with IGT, aged 55.5 years, and body mass index (BMI) 30.1 kg/m2.

Intervention:

Subjects received either placebo or 15 g AX supplement for 6 weeks with a 6-week washout period in-between.

Main outcome measurements:

Postprandial responses of serum glucose, insulin and triglycerides, and plasma total and acylated ghrelin after a liquid meal challenge test (LMCT) measured at the beginning and at the end of the dietary intervention at −20, −5, 0, 15, 30, 45, 60, 90, 120, 150, 180, 210 and 240 min.

Results:

After LMCT, AX consumption resulted in lower postprandial responses in serum glucose, insulin and triglycerides (P<0.05). Compared to placebo, total plasma ghrelin was also reduced by 42±8 pg/ml (P<0.001) after AX consumption with no difference in plasma acylated ghrelin.

Conclusion:

AX consumption improved postprandial metabolic responses after an LMCT in subjects with IGT and reduced total ghrelin response. However, acylated ghrelin responses were unchanged, suggesting that the acylated ghrelin-mediated orexigenic regulation is not improved as only total plasma ghrelin decreased.

Sponsorship:

Federal Ministry of Education and Research Germany (PTJ-BIO/0313042C).

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References

  • Amodo R, Neukom H (1985). Minor constituents of wheat flour: the pentosan. In: Hill R, Much, LDS (eds). New Approaches to Research on Cereal Carbohydrates. Elsevier Science Publishers: Amsterdam, pp 241–251.

    Google Scholar 

  • Anderson JW, Davidson MH, Blonde L, Brown WV, Howard WJ, Ginsberg H et al. (2000). Long-term cholesterol-lowering effects of psyllium as an adjunct to diet therapy in the treatment of hypercholesterolemia. Am J Clin Nutr 71, 1433–1438.

    Article  CAS  Google Scholar 

  • Asakawa A, Inui A, Fujimiya M, Sakamaki R, Shinfuku N, Ueta Y et al. (2005). Stomach regulates energy balance via acylated ghrelin and desacyl ghrelin. Gut 54, 18–24.

    Article  CAS  Google Scholar 

  • Benoit SC, Clegg DJ, Seeley RJ, Woods SC (2004). Insulin and leptin as adiposity signals. Recent Prog Horm Res 59, 267–285.

    Article  CAS  Google Scholar 

  • Brennan CS (2005). Dietary fibre, glycaemic response, and diabetes. Mol Nutr Food Res 49, 560–570.

    Article  Google Scholar 

  • Broglio F, Gottero C, Prodam F, Gauna C, Muccioli G, Papotti M et al. (2004). Non-acylated ghrelin counteracts the metabolic but not the neuroendocrine response to acylated ghrelin in humans. J Clin Endocrinol Metab 89, 3062–3065.

    Article  CAS  Google Scholar 

  • Brown L, Rosner B, Willett WW, Sacks FM (1999). Cholesterol-lowering effects of dietary fiber: a meta-analysis. Am J Clin Nutr 69, 30–42.

    Article  CAS  Google Scholar 

  • Callahan HS, Cummings DE, Pepe MS, Breen PA, Matthys CC, Weigle DS (2004). Postprandial suppression of plasma ghrelin level is proportional to ingested caloric load but does not predict intermeal interval in humans. J Clin Endocrinol Metab 89, 1319–1324.

    Article  CAS  Google Scholar 

  • Chandalia M, Garg A, Lutjohann D, von Bergmann K, Grundy SM, Brinkley LJ (2000). Beneficial effects of high dietary fiber intake in patients with type 2 diabetes mellitus. N Engl J Med 342, 1392–1398.

    Article  CAS  Google Scholar 

  • Chen CY, Inui A, Asakawa A, Fujino K, Kato I, Chen CC et al. (2005). Des-acyl ghrelin acts by CRF type 2 receptors to disrupt fasted stomach motility in conscious rats. Gastroenterology 129, 8–25.

    Article  CAS  Google Scholar 

  • Cowley MA, Smith RG, Diano S, Tschop M, Pronchuk N, Grove KL et al. (2003). The distribution and mechanism of action of ghrelin in the CNS demonstrates a novel hypothalamic circuit regulating energy homeostasis. Neuron 37, 649–661.

    Article  CAS  Google Scholar 

  • Dongowski G, Huth M, Gebhardt E (2003). Steroids in the intestinal tract of rats are affected by dietary-fibre-rich barley-based diets. Br J Nutr 90, 895–906.

    Article  CAS  Google Scholar 

  • Eastwood M, Kritchevsky D (2005). Dietary fiber: how did we get where we are? Annu Rev Nutr 25, 1–8.

    Article  CAS  Google Scholar 

  • Feinle-Bisset C, Patterson M, Ghatei MA, Bloom SR, Horowitz M (2005). Fat digestion is required for the suppression of ghrelin and stimulation of peptide YY and pancreatic polypeptide secretion by intraduodenal lipid. Am J Physiol Endocrinol Metab 289, E948–E953.

    Article  CAS  Google Scholar 

  • Fernandez ML (2001). Soluble fiber and nondigestible carbohydrate effects on plasma lipids and cardiovascular risk. Curr Opin Lipidol 12, 35–40.

    Article  CAS  Google Scholar 

  • Goel V, Cheema SK, Agellon LB, Ooraikul B, Basu TK (1999). Dietary rhubarb (Rheum rhaponticum) stalk fibre stimulates cholesterol 7 alpha-hydroxylase gene expression and bile acid excretion in cholesterol-fed C57BL/6J mice. Br J Nutr 81, 65–71.

    CAS  PubMed  Google Scholar 

  • Jang Y, Lee JH, Kim OY, Park HY, Lee SY (2001). Consumption of whole grain and legume powder reduces insulin demand, lipid peroxidation, and plasma homocysteine concentrations in patients with coronary artery disease: randomized controlled clinical trial. Arterioscler Thromb Vasc Biol 21, 2065–2071.

    Article  CAS  Google Scholar 

  • Koebnick C, Wagner K, Thielecke F, Dieter G, Hohne A, Franke A et al. (2005a). An easy-to-use semiquantitative food record validated for energy intake by using doubly labelled water technique. Eur J Clin Nutr 59, 989–995.

    Article  CAS  Google Scholar 

  • Koebnick C, Wagner K, Thielecke F, Moeseneder J, Hoehne A, Franke A et al. (2005b). Validation of a simplified physical activity record by doubly labeled water technique. Int J Obes Relat Metab Disord 29, 302–309.

    Article  CAS  Google Scholar 

  • Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K (1999). Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 402, 656–660.

    Article  CAS  Google Scholar 

  • Kojima M, Kangawa K (2002). Ghrelin, an orexigenic signaling molecule from the gastrointestinal tract. Curr Opin Pharmacol 2, 665–668.

    Article  CAS  Google Scholar 

  • Korbonits M, Goldstone AP, Gueorguiev M, Grossman AB (2004). Ghrelin – a hormone with multiple functions. Front Neuroendocrinol 25, 27–68.

    Article  CAS  Google Scholar 

  • Lavin JH, Read NW (1995). The effect on hunger and satiety of slowing the absorption of glucose: relationship with gastric emptying and postprandial blood glucose and insulin responses. Appetite 25, 89–96.

    Article  CAS  Google Scholar 

  • le Roux CW, Patterson M, Vincent RP, Hunt C, Ghatei MA, Bloom SR (2005). Postprandial plasma ghrelin is suppressed proportional to meal calorie content in normal-weight but not obese subjects. J Clin Endocrinol Metab 90, 1068–1071.

    Article  CAS  Google Scholar 

  • Liese AD, Roach AK, Sparks KC, Marquart L, D'Agostino Jr RB, Mayer-Davis EJ (2003). Whole-grain intake and insulin sensitivity: the Insulin Resistance Atherosclerosis Study. Am J Clin Nutr 78, 965–971.

    Article  CAS  Google Scholar 

  • Lu ZX, Gibson PR, Muir JG, Fielding M, O'Dea K (2000a). Arabinoxylan fiber from a by-product of wheat flour processing behaves physiologically like a soluble, fermentable fiber in the large bowel of rats. J Nutr 130, 1984–1990.

    Article  CAS  Google Scholar 

  • Lu ZX, Walker KZ, Muir JG, Mascara T, O'Dea K (2000b). Arabinoxylan fiber, a byproduct of wheat flour processing, reduces the postprandial glucose response in normoglycemic subjects. Am J Clin Nutr 71, 1123–1128.

    Article  CAS  Google Scholar 

  • Lu ZX, Walker KZ, Muir JG, O'Dea K (2004). Arabinoxylan fibre improves metabolic control in people with Type II diabetes. Eur J Clin Nutr 58, 621–628.

    Article  CAS  Google Scholar 

  • Lucidi P, Murdolo G, Di Loreto C, De Cicco A, Parlanti N, Fanelli C et al. (2002). Ghrelin is not necessary for adequate hormonal counterregulation of insulin-induced hypoglycemia. Diabetes 51, 2911–2914.

    Article  CAS  Google Scholar 

  • Lueder W, Noack R, Meuser F, Martens U, Gebhardt E, Rohde J (1996). Effect of physiologically active dietary fiber preparation from rye and wheat on postprandial glucose response [German]. Ernaehrungsumsch 43, 290–293.

    CAS  Google Scholar 

  • Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC (1985). Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28, 412–419.

    Article  CAS  Google Scholar 

  • McKeown NM, Meigs JB, Liu S, Wilson PW, Jacques PF (2002). Whole-grain intake is favorably associated with metabolic risk factors for type 2 diabetes and cardiovascular disease in the Framingham Offspring Study. Am J Clin Nutr 76, 390–398.

    Article  CAS  Google Scholar 

  • Meuser F, Imhof D (2002). Production of a dietary fibre concentrate recovered from wheat starch plant process water. Starch 54 (9), 421–434.

    Article  Google Scholar 

  • Mohlig M, Koebnick C, Weickert MO, Lueder W, Otto B, Steiniger J et al. (2005). Arabinoxylan-enriched meal increases serum ghrelin levels in healthy humans. Horm Metab Res 37, 303–308.

    Article  CAS  Google Scholar 

  • Nedvidkova J, Krykorkova I, Bartak V, Papezova H, Gold PW, Alesci S et al. (2003). Loss of meal-induced decrease in plasma ghrelin levels in patients with anorexia nervosa. J Clin Endocrinol Metab 88, 1678–1682.

    Article  CAS  Google Scholar 

  • Onning G, Wallmark A, Persson M, Akesson B, Elmstahl S, Oste R (1999). Consumption of oat milk for 5 weeks lowers serum cholesterol and LDL cholesterol in free-living men with moderate hypercholesterolemia. Ann Nutr Metab 43, 301–309.

    Article  CAS  Google Scholar 

  • Peeters TL (2005). Ghrelin: a new player in the control of gastrointestinal functions. Gut 54, 1638–1649.

    Article  CAS  Google Scholar 

  • Pereira MA, Jacobs Jr DR, Pins JJ, Raatz SK, Gross MD, Slavin JL et al. (2002). Effect of whole grains on insulin sensitivity in overweight hyperinsulinemic adults. Am J Clin Nutr 75, 848–855.

    Article  CAS  Google Scholar 

  • Pick ME, Hawrysh ZJ, Gee MI, Toth E, Garg ML, Hardin RT (1996). Oat bran concentrate bread products improve long-term control of diabetes: a pilot study. J Am Diet Assoc 96, 1254–1261.

    Article  CAS  Google Scholar 

  • Purnell JQ, Weigle DS, Breen P, Cummings DE (2003). Ghrelin levels correlate with insulin levels, insulin resistance, and high-density lipoprotein cholesterol, but not with gender, menopausal status, or cortisol levels in humans. J Clin Endocrinol Metab 88, 5747–5752.

    Article  CAS  Google Scholar 

  • Saad MF, Bernaba B, Hwu CM, Jinagouda S, Fahmi S, Kogosov E et al. (2002). Insulin regulates plasma ghrelin concentration. J Clin Endocrinol Metab 87, 3997–4000.

    Article  CAS  Google Scholar 

  • Schaller G, Schmidt A, Pleiner J, Woloszczuk W, Wolzt M, Luger A (2003). Plasma ghrelin concentrations are not regulated by glucose or insulin: a double-blind, placebo-controlled crossover clamp study. Diabetes 52, 16–20.

    Article  CAS  Google Scholar 

  • Schweizer TF, Wursch P (1991). The physiological and nutritional importance of dietary fibre. Experientia 47, 181–186.

    Article  CAS  Google Scholar 

  • Slavin JL (2005). Dietary fiber and body weight. Nutrition 21, 411–418.

    Article  Google Scholar 

  • Sparti A, Milon H, Di Vetta V, Schneiter P, Tappy L, Jequier E et al. (2000). Effect of diets high or low in unavailable and slowly digestible carbohydrates on the pattern of 24-h substrate oxidation and feelings of hunger in humans. Am J Clin Nutr 72, 1461–1468.

    Article  CAS  Google Scholar 

  • Strader AD, Woods SC (2005). Gastrointestinal hormones and food intake. Gastroenterology 128, 175–191.

    Article  CAS  Google Scholar 

  • Tremblay A, Boule N, Doucet E, Woods SC (2005). Is the insulin resistance syndrome the price to be paid to achieve body weight stability? Int J Obes (Lond) 29, 1295–1298.

    Article  CAS  Google Scholar 

  • Tremblay A, Doucet E (2000). Obesity: a disease or a biological adaptation? Obes Rev 1, 27–35.

    Article  CAS  Google Scholar 

  • Tschop M, Smiley DL, Heiman ML (2000). Ghrelin induces adiposity in rodents. Nature 407, 908–913.

    Article  CAS  Google Scholar 

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Correspondence to C Koebnick.

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Guarantor: C Koebnick.

Contributors: ALG, BO and CK were responsible for data analysis and writing of the manuscript. H-JFZ, FM and AHFP were responsible for the study design. SCR, AM and JS were involved in the collection and the analysis of the data. MOW, MM and NNR had an advisory function and contributed to the writing of the manuscript. BO, JD, NK and MS were responsible for laboratory analysis. All authors contributed to the manuscript. The authors do not have any financial or personal conflicts of interest.

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Garcia, A., Otto, B., Reich, SC. et al. Arabinoxylan consumption decreases postprandial serum glucose, serum insulin and plasma total ghrelin response in subjects with impaired glucose tolerance. Eur J Clin Nutr 61, 334–341 (2007). https://doi.org/10.1038/sj.ejcn.1602525

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